Pulsed Field Gel Electrophoresis (PFGE)

The Method of Pulsed Field Gel Electrophoresis (PFGE)

Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes … Read more

The Wobble Hypothesis

The Wobble Hypothesis

There are more than one codon for one amino acid. This is called degeneracy of genetic code. To explain the possible cause of degeneracy of codons, in 1966, Francis Crick … Read more

Restriction Enzyme (Restriction Endonuclease)

Restriction Enzyme (Restriction Endonuclease)

Restriction Enzyme (Restriction Endonuclease) Definition Restriction enzyme, also called restriction endonuclease, is a protein produced by bacteria that cleaves DNA at specific sites along the molecule. Restriction endonucleases cut the DNA double helix in very … Read more

DNA Microarray

Steps Involved in cDNA based Microarray

DNA microarrays are solid supports, usually of glass or silicon, upon which DNA is attached in an organized pre-determined grid fashion. Each spot of DNA, called a probe, represents a … Read more