Sudan Black B Staining

Sudan Black B Staining

Sudan Black B Staining definition Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan … Read more

Masson’s Trichrome Staining

Masson's Trichrome Staining

Masson’s Trichrome Staining definition Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin … Read more

Wheatley Trichrome Staining

Wheatley Trichrome Staining

Wheatley Trichrome Staining definition Wheatley Trichrome staining technique is a special permanent satin used in parasitology for the detection and identification of intestine protozoans from stool samples. Trichrome staining is performed on a PVA-fixed or Schaudinn’s- Preserved stool sample. The … Read more

Positive staining of Viruses

Positive staining of Viruses

What is positive staining? Positive staining of viruses is similar to negative staining. The difference is that the virus image is darker formed on a light background, unlike the negative staining where a light viral particle image is formed on … Read more

Romanowsky Stains- Principle, Types, Applications

Romanowsky Stains

What are the Romanowsky Stains? Romanowsky Stains are the stains that are used in hematology and cytological studies, to differentiate cells in microscopic examinations of blood and bone marrow samples. These stains are also applied to detect the presence of … Read more

Periodic Acid-Schiff (PAS) Staining

Periodic Acid-Schiff (PAS) Staining

What is Periodic Acid-Schiff (PAS) Staining? Periodic-Acid Schiff (PAS) staining technique is used in histochemistry and histological studies to demonstrate the presence of carbohydrates and carbohydrate compounds such as polysaccharides, mucin, glycogen, and fungal cell wall components. It has been … Read more

Negative staining of Viruses

Negative staining of Viruses

What is the Negative staining of Viruses? Staining techniques are designed to show contrast under microscopic observation. The staining of viruses is done to increase the contrast between the virus and the background. The use of an electron microscope intensifies … Read more

Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide Staining

What is Collagen Hybridizing Peptide Staining? Collagen is the major building block of all tissues including tendon, ligament, cornea, cartilage, and bone It is a fibrous, structural protein that made up of a right-handed bundle of three parallel, left-handed polyproline II-type … Read more

Iron-Hematoxylin Staining

Iron-Hematoxylin Staining

What is Iron-Hematoxylin Staining? This is a traditional staining technique commonly used for the detection of intestinal protozoans majorly because of its permanence and ability to demonstrate, observe, and quantify with high clarity protozoans nuclear structures. Iron hematoxylin was the stain used … Read more

Auramine- Rhodamine Staining

Auramine- Rhodamine Staining

What is Auramine- Rhodamine Staining? The evolution in staining methodologies has led to an era of modified staining techniques that are rapid, more versatile, and reliable in result interpretation. This is one of those techniques which was modified from the … Read more

Lactophenol Cotton Blue (LPCB) Staining

Lactophenol Cotton Blue (LPCB) Staining

Lactophenol Cotton Blue (LPCB) Staining is a simple histological staining method used for the microscopic examination and identification of fungi. Principle of Lactophenol Cotton Blue (LPCB) Staining Lactophenol Cotton Blue (LPCB) Staining method works on the principle of aiding the … Read more

Hematoxylin and eosin stain (H and E stain or HE stain)

Hematoxylin and eosin stain (H&E stain or HE stain)

Hematoxylin and eosin staining technique functions to recognize different types of tissues and their morphological changes, especially in cancer diagnosis. Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and … Read more

Calcofluor White Staining

Calcofluor White Staining

Calcofluor White Staining Calcofluor white is a chemifluorescent blue dye that is nonspecifically used to bind to the beta linked-polysaccharide polymers of amoebic cysts. It functions by being able to bind to 1-3 beta and 1-4 beta polysaccharides on chitin … Read more

Grocott-Gomori’s Methenamine Silver Staining

Grocott-Gomori's Methenamine Silver Staining

Grocott-Gomori’s Methenamine Silver (GMS) stain is a histological stain that is used majorly for the identification of carbohydrates in fungal microorganisms. This staining method was named after György Gömöri, a physician from Hungary, who developed the staining methodology. Its initial … Read more