Sudan Black B Staining

Sudan Black B Staining

Sudan Black B Staining definition Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan groups of dyes are defined as lysochrome dyes which are soluble in fats and lipids and also lipid solvents. Structurally …

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Masson’s Trichrome Staining

Masson's Trichrome Staining

Masson’s Trichrome Staining definition Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin solution, and Aniline blue. Objectives of Masson’s Trichrome staining To stain the collage fibers. To stain collagen. To stain keratin. …

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Wheatley Trichrome Staining

Wheatley Trichrome Staining

Wheatley Trichrome Staining definition Wheatley Trichrome staining technique is a special permanent satin used in parasitology for the detection and identification of intestine protozoans from stool samples. Trichrome staining is performed on a PVA-fixed or Schaudinn’s- Preserved stool sample. The protozoan morphologies identified are cysts and trophozoites. Gomori developed this stain in 1951 and it was late modified by Wheatley …

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Positive staining of Viruses

Positive staining of Viruses

What is positive staining? Positive staining of viruses is similar to negative staining. The difference is that the virus image is darker formed on a light background, unlike the negative staining where a light viral particle image is formed on a dark background. The technique is simple and it is used widely to study the diverse morphologies of viruses from …

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Romanowsky Stains- Principle, Types, Applications

Romanowsky Stains

What are the Romanowsky Stains? Romanowsky Stains are the stains that are used in hematology and cytological studies, to differentiate cells in microscopic examinations of blood and bone marrow samples. These stains are also applied to detect the presence of parasites in the blood such as malaria parasites. There are various Romanowsky staining types that apply the same principle. They …

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Periodic Acid-Schiff (PAS) Staining

Periodic Acid-Schiff (PAS) Staining

What is Periodic Acid-Schiff (PAS) Staining? Periodic-Acid Schiff (PAS) staining technique is used in histochemistry and histological studies to demonstrate the presence of carbohydrates and carbohydrate compounds such as polysaccharides, mucin, glycogen, and fungal cell wall components. It has been used to detect glycogen in tissues such as the skeletal muscles, the liver, the cardiac muscles. It uses formalin-fixed, paraffin-embedded …

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Negative staining of Viruses

Negative staining of Viruses

What is the Negative staining of Viruses? Staining techniques are designed to show contrast under microscopic observation. The staining of viruses is done to increase the contrast between the virus and the background. The use of an electron microscope intensifies the density of electron toward the stain. Negative staining is one of the most excellent techniques in studying viruses, bacterial …

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Silver Staining- Principle, Procedure, Applications

Silver Staining

What is silver staining? Silver staining is a special yet powerful staining technique that is used for the detection and identification of proteins in gels. This is because silver binds to the chemical terminal or side chains of amino groups i.e carboxyl and sulfhydryl groups. It has been used for decades now to separate proteins from polyacrylamide gel electrophoresis. The …

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Collagen Hybridizing Peptide Staining

Collagen Hybridizing Peptide Staining

What is Collagen Hybridizing Peptide Staining? Collagen is the major building block of all tissues including tendon, ligament, cornea, cartilage, and bone It is a fibrous, structural protein that made up of a right-handed bundle of three parallel, left-handed polyproline II-type helices. Collagen can undergo extensive proteolytic remodeling during development causing collagen degradation leading to a variety of life-threatening diseases such …

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Iron-Hematoxylin Staining

Iron-Hematoxylin Staining

What is Iron-Hematoxylin Staining? This is a traditional staining technique commonly used for the detection of intestinal protozoans majorly because of its permanence and ability to demonstrate, observe, and quantify with high clarity protozoans nuclear structures. Iron hematoxylin was the stain used for most of the original morphological descriptions of intestinal protozoa found in humans. The iron hematoxylin staining method can stain …

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