Staining is a biochemical process usually conducted to increase the contrast of a microscopic image.
Sudan Black B Staining definition Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan … Read more
Masson’s Trichrome Staining definition Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin … Read more
Wheatley Trichrome Staining definition Wheatley Trichrome staining technique is a special permanent satin used in parasitology for the detection and identification of intestine protozoans from stool samples. Trichrome staining is performed on a PVA-fixed or Schaudinn’s- Preserved stool sample. The … Read more
What is positive staining? Positive staining of viruses is similar to negative staining. The difference is that the virus image is darker formed on a light background, unlike the negative staining where a light viral particle image is formed on … Read more
What are the Romanowsky Stains? Romanowsky Stains are the stains that are used in hematology and cytological studies, to differentiate cells in microscopic examinations of blood and bone marrow samples. These stains are also applied to detect the presence of … Read more
What is Periodic Acid-Schiff (PAS) Staining? Periodic-Acid Schiff (PAS) staining technique is used in histochemistry and histological studies to demonstrate the presence of carbohydrates and carbohydrate compounds such as polysaccharides, mucin, glycogen, and fungal cell wall components. It has been … Read more
What is the Negative staining of Viruses? Staining techniques are designed to show contrast under microscopic observation. The staining of viruses is done to increase the contrast between the virus and the background. The use of an electron microscope intensifies … Read more
What is silver staining? Silver staining is a special yet powerful staining technique that is used for the detection and identification of proteins in gels. This is because silver binds to the chemical terminal or side chains of amino groups … Read more
What is Collagen Hybridizing Peptide Staining? Collagen is the major building block of all tissues including tendon, ligament, cornea, cartilage, and bone It is a fibrous, structural protein that made up of a right-handed bundle of three parallel, left-handed polyproline II-type … Read more
What is Iron-Hematoxylin Staining? This is a traditional staining technique commonly used for the detection of intestinal protozoans majorly because of its permanence and ability to demonstrate, observe, and quantify with high clarity protozoans nuclear structures. Iron hematoxylin was the stain used … Read more
What is Auramine- Rhodamine Staining? The evolution in staining methodologies has led to an era of modified staining techniques that are rapid, more versatile, and reliable in result interpretation. This is one of those techniques which was modified from the … Read more
What is Papanicolaou Staining (Pap stain)? Papanicolaou stain is also known as the pap stain and the procedure of the stain is known as a pap smear. It is a polychromatic stain that uses multiple dyes to differentially stain various … Read more
What is Ziehl-Neelsen staining? The Ziehl-Neelsen staining technique is a differential staining technique that was initially developed by Ziehl and modified later by Neelsen, hence the name Ziehl-Neelsen stain. Neelsen used carbol-fuschin from Ziehl’s experiment, with heat and added a … Read more
Lactophenol Cotton Blue (LPCB) Staining is a simple histological staining method used for the microscopic examination and identification of fungi. Principle of Lactophenol Cotton Blue (LPCB) Staining Lactophenol Cotton Blue (LPCB) Staining method works on the principle of aiding the … Read more
Hematoxylin and eosin staining technique functions to recognize different types of tissues and their morphological changes, especially in cancer diagnosis. Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and … Read more
Calcofluor White Staining Calcofluor white is a chemifluorescent blue dye that is nonspecifically used to bind to the beta linked-polysaccharide polymers of amoebic cysts. It functions by being able to bind to 1-3 beta and 1-4 beta polysaccharides on chitin … Read more
Grocott-Gomori’s Methenamine Silver (GMS) stain is a histological stain that is used majorly for the identification of carbohydrates in fungal microorganisms. This staining method was named after György Gömöri, a physician from Hungary, who developed the staining methodology. Its initial … Read more
Introduction Giemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. It belongs to a group of stains known as Romanowsky stains. These … Read more
The Gram stain was developed by Christian Gram in 1884 and modified by Hucker in 1921. The objective of Gram Stain This test differentiates the bacteria into Gram-Positive and Gram-Negative Bacteria, which helps in the classification and differentiation of microorganisms. … Read more
Special stains have been developed over time for identifying bacteria species, differentiating them morphologically, and even characterizing there very special features. The most common stain being Gram Staining, Acid-fast staining, endospore staining. Each of these stains aims at identifying and … Read more
Introduction A normal bacterial cell is known as a vegetative form of a bacterial cell. It can multiply freely when provided with conducive environmental conditions like the provision of nutrients, temperature, water, and oxygen. But some groups of bacteria, when … Read more