Staining

Flagella Stain- Principle, Procedure and Result Interpretation

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Flagella Stain- Principle, Procedure and Result Interpretation

Flagella Stain- Principle, Procedure and Result Interpretation Objective of Flagella Stain This technique is used to visualize the presence and arrangement of flagella for the presumptive identification of motile bacterial species. Principle of Flagella Stain Flagella are too thin to be visualized using a bright field microscope with ordinary stains, such as the Gram stain, … Read more Flagella Stain- Principle, Procedure and Result Interpretation

Spore Stain- Principle, Procedure and Result Interpretation

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Spore Stain- Principle, Procedure and Result Interpretation

Spore Stain- Principle, Procedure and Result Interpretation Members of the anaerobic genera Clostridium and Desulfotomaculum and the aerobic genus Bacillus are examples of organisms that have the capacity to exist either as metabolically active vegetative cells or as highly resistant, metabolically inactive cell types called spores. When environmental conditions become unfavorable for continuing vegetative cellular … Read more Spore Stain- Principle, Procedure and Result Interpretation

Capsule Stain- Principle, Procedure and Result Interpretation

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Capsule Stain- Principle, Procedure and Result Interpretation A capsule is a gelatinous outer layer that is secreted by the cell and that surrounds and adheres to the cell wall. It is not common to all organisms. Cells that have a heavy capsule are generally virulent and capable of producing disease, since the structure protects bacteria … Read more Capsule Stain- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation

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Negative Staining- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation Objectives of Negative Staining To perform a negative staining procedure. To understand the benefit obtained from visualizing unstained microorganisms. Principle of Negative Staining Negative staining requires the use of an acidic stain such as India ink or nigrosin. The acidic stain, with its negatively charged chromogen, will not … Read more Negative Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation

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Simple Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation Objectives of Simple Staining To perform a simple staining procedure. To compare the morphological shapes and arrangements of bacterial cells. Principle of Simple Staining In simple staining, the bacterial smear is stained with a single reagent, which produces a distinctive contrast between the organism and its background. Basic stains with … Read more Simple Staining- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

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Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a … Read more Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

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Acid Fast (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. … Read more Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

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Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells … Read more Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Gram Stain- Principle, Reagents, Procedure and Result Interpretation

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Gram Stain- Principle, Reagents, Procedure and Result Interpretation

Gram Stain- Principle, Reagents, Procedure and Result Interpretation The Gram stain was developed by Christian Gram in 1884 and modified by Hucker in 1921. Objective of Gram Stain This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiation of microorganisms. The Gram stain separates bacteria into two … Read more Gram Stain- Principle, Reagents, Procedure and Result Interpretation