Anupama Sapkota

I have done my BSc. in Microbiology from St. Xavier's College, Maitighar, Nepal. I am particularly interested in studies regarding antibiotic resistance with a focus on drug discovery.

MCH Blood Test- Definition, Calculation, Results, High & Low MCH value

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What is MCH Blood Test? MCH (Mean Corpuscular Haemoglobin) test is a test performed to calculate the average amount of hemoglobin present in the red blood cells of a blood sample. Hemoglobin is an essential protein found in the blood as it is responsible for the transport of gases and nutrients throughout the body. MCH is different from MCHC even …

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MPV Blood Test- Definition, Calculation, Results, High & Low MPV value

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What is MPV Blood Test? MPV (Mean Platelet Volume) test is a test performed to determine the average size of platelets present in a blood sample. Platelets are important blood cells that are responsible for blood clotting, a process that controls blood loss after an injury. The mean platelet volume (MPV) is the measurement of the dimension of platelets, calculated …

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Buffy Coat- Definition, Preparation, Uses

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Buffy Coat Definition A buffy coat suspension is a concentrated suspension of leukocytes and platelets that make up a part of the anticoagulated blood sample obtained by the process of density gradient centrifugation. The term buffy coat arose from the fact that the suspension has a color (yellowish beige) that is similar to buff. Buffy coats primarily contain white blood …

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Blood Cells- Definition and Types with Structure and Functions

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Blood Cells Definition Blood cells, also known as hematocytes, hemocytes, or hematopoietic cells, are cells produced mostly in the blood and are synthesized primarily in the red bone marrow. Blood cells make up about 45% of the blood volume, while the rest (55%) is occupied by blood plasma. Blood contains three different types of blood cells, namely, red blood cell …

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T-test- definition, formula, types, applications, example

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T-test definition The t-test is a test in statistics that is used for testing hypotheses regarding the mean of a small sample taken population when the standard deviation of the population is not known. The t-test is used to determine if there is a significant difference between the means of two groups. The t-test is used for hypothesis testing to …

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Urease Test- Principle, Media, Procedure, Result, Uses

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Urease Test Definition Urease test is a biochemical test that detects the alkaline fermentation of urine (urea) with the resultant production of ammonia by microorganisms. The fermentation of urea occurs in the presence of the enzyme ‘urease’, resulting in two molecules of ammonia and carbon dioxide. Urease activity is one of the important characteristics for the identification of Proteus species …

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Indole Test- Principle, Media, Procedure, Types, Results, Uses

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Indole Test Definition Indole test is a biochemical test conducted on bacterial species to detect their ability to produce indole from tryptophan in the presence of a group of enzymes called ‘tryptophanase’. It is a qualitative test that tests the conversion of tryptophan into indole. The test is performed as a part of the IMViC test that is used to …

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Hippurate Hydrolysis Test- Principle, Procedure, Result, Uses

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Hippurate Hydrolysis Test Definition Hippurate hydrolysis test is a biochemical test that differentiates microorganisms on the basis of their ability to hydrolyze hippurate into glycine and benzoic acid by the enzymatic action of hippuricase. Traditionally, the test was performed using a ferric chloride indicator to detect benzoic acid, which would take a longer period of time. Nowadays, however, the test …

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Growth at 42°C Test- Principle, Procedure, Result, Uses

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What is Growth at 42°C Test? The growth at 42°C test is a presumptive identification test used to differentiate between the species of the fluorescent pseudomonas group as well as other non-fermentative bacteria. The test is based on the ability of an organism to grow at 42°C, which allows the differentiation of Pseudomonas aeruginosa from the other fluorescent pseudomonads. Temperature …

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Gelatin Hydrolysis Test- Principle, Procedure, Types, Result, Uses

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Gelatin Hydrolysis Test Definition Gelatin hydrolysis test is a biochemical test performed as a presumptive test for the identification of Staphylococcus, Enterococcus, and other Gram-positive bacilli. Gelatin hydrolysis test is also termed as the Gelatin Liquefaction test as it involves the liquefaction of gelatin in the presence of the gelatinase enzyme. Gelatinase is an important enzyme in various pathogenic organisms …

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