Brain Lipid Extraction Protocol
“Brain lipid extracts” refers to a lipid mixture extracted from the brain tissue of animals. It has been found that such brain lipid extracts contain a mixture of long chain … Read more
Protocols
Microbiology Protocols. Molecular Biology and Genetics Protocols. Biology and Life Sciences Protocols for undergraduate and graduate students with diagrams.
Protocol of chloroplast isolation
The chloroplast is an important organelle found in plant cells that conduct photosynthesis. It is enclosed by a pair of closely spaced membranes, the double-membrane envelope, consisting of the inner … Read more
Protocol: Phenol-chloroform extraction of prokaryotic DNA
Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. The simplest … Read more
Rapid Method for Enumeration of E. coli Biotype I
This method is modified by Anderson and Parker. Prepare serial tenfold dilutions in peptone water of food. Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar. … Read more
Oxygen Release in Photosynthesis Protocol
Photosynthesis is the biological process that converts sunlight into chemical energy. It is employed by plants, algae and certain bacteria. It provides the basis for life on Earth and is … Read more
Isolation of Mitochondria from Plants, Yeast Cells, Mice, Cell Culture
Mitochondria are central organelles controlling the life and death of the cell. They participate in key metabolic reactions, synthesize most of the ATP and regulate a number of signaling cascades, … Read more
Eukaryotic DNA Isolation Protocol
Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. In eukaryotic … Read more
Bacterial Growth Curve Protocol
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria. If the bacterial … Read more
RNA Isolation Protocol
Total RNA isolation from Bacterial Cells Principle of RNA Isolation Total RNA is isolated and separated from DNA and protein after extraction with a solution called as Trizol. Trizol is … Read more