Lineweaver–Burk Plot Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from
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Category: Biochemistry
Ethanol Metabolism
Ethanol Metabolism Alcohol is metabolized by several processes or pathways. The most common of these pathways involves two enzymes—alcohol dehydrogenase
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Brain Lipid Extraction Protocol
Brain Lipid Extraction Protocol “Brain lipid extracts” refers to a lipid mixture extracted from the brain tissue of animals. It
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Glycogenolysis
Glycogenolysis Glycogen is the major storage form of carbohydrate in animals similar to starch in plants. It is a homopolymer
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De novo pyrimidine synthesis
De novo pyrimidine synthesis Biosynthesis of pyrimidine nucleotides can occur by a de novo pathway or by the reutilization of preformed pyrimidine bases
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Alpha Oxidation
Alpha Oxidation Alpha – oxidation is defined as the oxidation of fatty acid (methyl group at beta carbon) with the
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Glycogenesis
Glycogenesis Glycogen is the major storage form of carbohydrate in animals similar to starch in plants. It is a homopolymer
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Enzyme Inhibition
Enzyme Inhibition Enzymes are the biological macromolecules, also called as biological catalysts, which speed up the rate of biochemical reactions without
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The Michaelis–Menten model
The Michaelis–Menten model A major role of proteins is to serve as enzymes, the catalysts of biochemical reactions. They do
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Buffer and Extraction Buffer
Buffer and Extraction Buffer A buffer is an aqueous solution consisting of a mixture of a weak acid and its
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