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Biotechnology

Biotechnology is a branch of biology that deals with the utilization of living beings or living processes for the production of different products that are important for living beings.

  • Biotechnology is a fairly emerging discipline that crosses technical processes like manufacturing with biological compounds.
  • The discipline utilizes both cellular and biomolecular processes to develop processes and products that improve our lives and the biological system of the planet.
  • Biotechnology often overlaps with other fields like immunology, genomics, recombinant technology, and pharmaceutical microbiology.
  • Even though biotechnology is viewed as a rather modern concept, it has been widely used throughout history in the form of increasing cultivation, breeding, and therapies.
  • In modern times, however, biotechnology makes use of more complex concepts like recombinant DNA technology, plant tissue culture, among others.
  • Biotechnology exists in a close relationship with bioinformatics for the exploration, extraction, and production of biological products.
  • The production of insulin and antibiotics is one of the most notable examples of biotechnology that supports living beings and protects them from various diseases.
  • One of the first uses of biotechnology is in the agricultural area where farmers employed techniques of selective breeding in order to increase the harvest.
  • Besides, the production of alcohol and bread by the process of fermentation is another important and commonly used biotechnological technique.
  • Four of the most common applications of biotechnology in modern times are made in areas like medicine, agriculture, industries, and the environment.
  • Different forms of biotechnological processes like wastewater management are considered imperative for the protection of water sources.
  • Plant and animal tissue culture has given new ways to form life which solves the problem of infertility in both plants and animals.
  • A branch of biotechnology called, biopharmaceuticals are involved in the utilization of living beings for the production of different therapeutic agents that help in the treatment of life-threatening diseases.

RNA Isolation Using the TRIzol Method: Principle, Steps, Applications

July 19, 2026July 5, 2026 by Sandeep Shakya
RNA Isolation Using the TRIzol Method

The TRIzol-based RNA isolation method is derived from the classical acid guanidinium thiocyanate-phenol-chloroform extraction technique first described by Chomczynski and Sacchi (1987). This method was specifically developed for the rapid … Read more

Protein Extraction from Bacterial Cells: Principle, Steps, Applications

July 19, 2026July 3, 2026 by Sandeep Shakya
Protein extraction from bacterial cells

Protein extraction from bacterial cells is a fundamental step in molecular biology, biotechnology, and proteomics research. The process involves disrupting the bacterial cell envelope to release intracellular proteins into a … Read more

Paper-Based Method of DNA Extraction: Principle, Steps, Uses

July 18, 2026July 1, 2026 by Sandeep Shakya
Paper-Based Method of DNA Extraction

Paper-based DNA extraction is an innovative solid-phase approach that utilizes porous paper matrices, primarily cellulose or cellulose-derived substrates, to isolate nucleic acids from biological samples. Unlike conventional extraction methods that … Read more

Phenol-Chloroform Method of DNA Extraction: Principle, Steps, Applications

July 17, 2026June 30, 2026 by Sandeep Shakya
Phenol-Chloroform Method of DNA Extraction

The phenol-chloroform method of DNA extraction is the technique that separates nucleic acids from proteins and other cellular components by exploiting their different solubilities in water and organic solvents. After … Read more

Spin Column-Based Method of DNA Extraction: Principle, Steps, Uses

July 17, 2026June 29, 2026 by Sandeep Shakya
Spin Column-Based Method of DNA Extraction

The spin column-based method is a commonly used DNA extraction technique in molecular biology laboratories. The Spin Column-Based Method of DNA Extraction works by allowing DNA to bind selectively to … Read more

Salting-Out Method of DNA Extraction: Principle, Steps, Uses

July 11, 2026June 28, 2026 by Sandeep Shakya
Salting-Out Method of DNA Extraction

The salting-out method of DNA Extraction is a simple and widely used technique for extracting genomic DNA from various cell types, including blood cells and tissue samples. Instead of relying … Read more

Chelex Method of DNA Extraction: Principle, Steps, Uses

July 10, 2026June 27, 2026 by Sandeep Shakya
Chelex Method of DNA Extraction

The Chelex method of DNA extraction is a rapid, simple, and cost-effective technique primarily developed to obtain PCR-ready DNA from biological samples. First introduced for forensic applications by Walsh et … Read more

DNA Quantification Using Nanodrop: Principle, Steps, Uses

July 11, 2026June 26, 2026 by Sandeep Shakya
DNA Quantification Using Nanodrop

DNA quantification is a fundamental step in molecular biology, ensuring that downstream applications such as PCR, qPCR, sequencing, cloning, and restriction digestion are performed with optimal template input. Among the … Read more

Boiling Method of DNA Extraction: Principle, Steps, Applications

July 12, 2026June 25, 2026 by Sandeep Shakya
Boiling Method of DNA Extraction

The boiling method of DNA extraction is one of the simplest, fastest, and most cost-effective procedures for isolating nucleic acids from biological samples. Unlike conventional chemical extraction methods, such as … Read more

Manual Plasmid Isolation (Alkaline Lysis): Principle, Reagents, Steps, Uses

June 22, 2026June 7, 2026 by Susmita Baniya
Manual Plasmid Isolation

Manual plasmid isolation is the hands-on laboratory procedure used to extract and purify plasmid DNA from cells such as bacterial and yeast cells, typically at a laboratory scale for research … Read more

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