Sudan Black B Staining

Sudan Black B Staining definition Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan groups of dyes are defined as lysochrome dyes which are soluble in fats and lipids and also lipid solvents. Structurally …

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Masson’s Trichrome Staining

Masson’s Trichrome Staining definition Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin solution, and Aniline blue. Objectives of Masson’s Trichrome staining To stain the collage fibers. To stain collagen. To stain keratin. …

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Wheatley Trichrome Staining

Wheatley Trichrome Staining definition Wheatley Trichrome staining technique is a special permanent satin used in parasitology for the detection and identification of intestine protozoans from stool samples. Trichrome staining is performed on a PVA-fixed or Schaudinn’s- Preserved stool sample. The protozoan morphologies identified are cysts and trophozoites. Gomori developed this stain in 1951 and it was late modified by Wheatley …

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T-test- definition, formula, types, applications, example

T-test definition The t-test is a test in statistics that is used for testing hypotheses regarding the mean of a small sample taken population when the standard deviation of the population is not known. The t-test is used to determine if there is a significant difference between the means of two groups. The t-test is used for hypothesis testing to …

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Urease Test- Principle, Media, Procedure, Result, Uses

Urease Test Definition Urease test is a biochemical test that detects the alkaline fermentation of urine (urea) with the resultant production of ammonia by microorganisms. The fermentation of urea occurs in the presence of the enzyme ‘urease’, resulting in two molecules of ammonia and carbon dioxide. Urease activity is one of the important characteristics for the identification of Proteus species …

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Indole Test- Principle, Media, Procedure, Types, Results, Uses

Indole Test Definition Indole test is a biochemical test conducted on bacterial species to detect their ability to produce indole from tryptophan in the presence of a group of enzymes called ‘tryptophanase’. It is a qualitative test that tests the conversion of tryptophan into indole. The test is performed as a part of the IMViC test that is used to …

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Hippurate Hydrolysis Test- Principle, Procedure, Result, Uses

Hippurate Hydrolysis Test Definition Hippurate hydrolysis test is a biochemical test that differentiates microorganisms on the basis of their ability to hydrolyze hippurate into glycine and benzoic acid by the enzymatic action of hippuricase. Traditionally, the test was performed using a ferric chloride indicator to detect benzoic acid, which would take a longer period of time. Nowadays, however, the test …

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Growth at 42°C Test- Principle, Procedure, Result, Uses

What is Growth at 42°C Test? The growth at 42°C test is a presumptive identification test used to differentiate between the species of the fluorescent pseudomonas group as well as other non-fermentative bacteria. The test is based on the ability of an organism to grow at 42°C, which allows the differentiation of Pseudomonas aeruginosa from the other fluorescent pseudomonads. Temperature …

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Gelatin Hydrolysis Test- Principle, Procedure, Types, Result, Uses

Gelatin Hydrolysis Test Definition Gelatin hydrolysis test is a biochemical test performed as a presumptive test for the identification of Staphylococcus, Enterococcus, and other Gram-positive bacilli. Gelatin hydrolysis test is also termed as the Gelatin Liquefaction test as it involves the liquefaction of gelatin in the presence of the gelatinase enzyme. Gelatinase is an important enzyme in various pathogenic organisms …

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Esculin Hydrolysis Test- Principle, Procedure, Results, Uses

What is the Esculin Hydrolysis Test? Esculin hydrolysis is a useful test in the differentiation of both gram-positive and gram-negative bacteria covering a broad spectrum of aerobes, facultative anaerobes, and anaerobes. Esculin hydrolysis is utilized as a taxonomic tool in the identification of a wide variety of microorganisms, including the family Enterobacteriaceae, genera Streptococcus and Listeria, non-fermentative gram-negative bacilli, and …

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