Online microbiology notes by SAGAR ARYAL
Peptone Yeast Extract Glucose Broth Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K to facilitate the recovery of the more fastidious organisms such as Prevotella spp, Porphyromonas spp, and the Bacteroides fragilis group. With the addition of a specific biochemical, the Peptone Yeast Extract Broth Based Media are used to determine … Read morePeptone Yeast Extract Glucose Broth
Streptococcus Selective Agar Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness of hemolytic zones produced by these organisms. Streptococcus Selective Agar is a selective medium recommended for use in the primary isolation of … Read moreStreptococcus Selective Agar
Lactobacilli MRS Broth MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for a number of fastidious strains which grow poorly on other general media. … Read moreLactobacilli MRS Broth
Cystine Tryptic Agar Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods when stored at appropriate temperatures. It is used for the detection of bacterial motility and, with added carbohydrate, it contributes to fermentation … Read moreCystine Tryptic Agar
Cystine Glucose Blood Agar Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., cystine). F. tularensis is a fastidious organism and requires cysteine for best growth. To recognize the lifetime achievements of Francis in understanding … Read moreCystine Glucose Blood Agar
Plate Count Agar (PCA) Plate count agar (PCA) is a bacteriological substrate used for determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium. The amount of bacteria is expressed as colony forming units per gram (CFU/g), in solid samples and per ml (CFU/ml) in liquid samples. … Read morePlate Count Agar (PCA)
Middlebrook Agar There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. Later, Dubos and Middlebrook developed various formulations containing oleic acid and albumin as key ingredients, which protect Mycobacterium from toxic agents, helping for … Read moreMiddlebrook Agar
Loeffler Medium Loeffler Medium is a modification of the original formula developed by Loeffler in 1887. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms, especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or … Read moreLoeffler Medium
Thioglycollate Broth Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. Image Source: Prince George’s Community College Composition of Thioglycollate Broth Ingredients Gms/litre L-cystine … Read moreThioglycollate Broth
Yeast Extract Peptone Dextrose (YPD or YEPD) Agar Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. They are chemoorganotrophs as they utilize organic compounds as a source of energy. Yeast extract peptone dextrose (YPD or YEPD) Growth Agar is used for the maintenance and propagation of yeasts including S. cerevisiae in … Read moreYeast Extract Peptone Dextrose (YPD or YEPD) Agar