New York City Agar

New York City Agar

Introduction NYC Agar Base medium was originally developed by Fauer, Weisburd and Wilson at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens. NYC medium, primarily designed for isolation of pathogenic Neisseria, also … Read more

Martin Lewis Agar

Martin Lewis Agar

Introduction Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. A further modification of these early formulations was developed to improve the isolation of pathogenic Neisseria sp. from specimens, which contained large amounts of … Read more

Modified Thayer Martin Agar

Modified Thayer Martin Agar

Thayer and Martin reported an improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin, colistin and nystatin have been added to minimize overgrowth by contaminants, suppress the growth of saprophytic Neisseria sp., and enhance the growth of pathogenic Neisseria. … Read more

Peptone Water

Peptone Water

Peptone water is formulated as per Shread, Donovan, and Lee. It is a broth medium used for the growth of the organism and a base for determining carbohydrate fermentation patterns of non-fastidious organisms. In addition, it is also used for … Read more

Sorbitol MacConkey Agar

Sorbitol MacConkey Agar

Introduction MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting Escherichia coli serotype O157:H7 associated with hemorrhagic colitis. E. coli serotype O157:H7 is a human … Read more

Peptone Yeast Extract Glucose Broth

Peptone Yeast Extract Glucose Broth

Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K to facilitate the recovery of the more fastidious organisms such as Prevotella spp, Porphyromonas spp, and the Bacteroides fragilis group. With the addition of a specific biochemical, the … Read more

Streptococcus Selective Agar

Streptococcus Selective Agar

Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness of hemolytic zones produced by these organisms. Streptococcus Selective Agar … Read more

Lactobacilli MRS Broth

Lactobacilli MRS Broth

MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for … Read more

Cystine Tryptic Agar

Cystine Tryptic Agar

Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods when stored at appropriate temperatures. It is used for the … Read more

Cystine Glucose Blood Agar

Francisella tularensis on Cystine Glucose Blood Agar

Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., cystine). F. tularensis is a fastidious organism and requires cysteine for … Read more

Plate Count Agar (PCA)

Plate Count Agar

Plate count agar (PCA) is a bacteriological substrate used for determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium.  The amount of bacteria is expressed as colony forming units per gram … Read more

Middlebrook Agar

Middlebrook Agar

There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. Later, Dubos and Middlebrook developed various formulations containing oleic acid … Read more

Loeffler Medium

Loeffler Medium

Loeffler Medium is a modification of the original formula developed by Loeffler in 1887. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms, especially from nose and throat. It also restores virulence and other identifying properties … Read more

Thioglycollate Broth

Thioglycollate Broth

Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. … Read more

Yeast Extract Peptone Dextrose (YPD or YEPD) Agar

Yeast Extract Peptone Dextrose (YPD or YEPD) Agar

Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. They are chemoorganotrophs as they utilize organic compounds as a source of energy. Yeast extract peptone dextrose (YPD or YEPD) Growth Agar is used for the maintenance and … Read more

Yersinia Selective Agar

Yersinia Selective Agar

Yersinia Selective Agar was first described by Schiemann as an alternative to MacConkey Agar and other commonly used media for isolation of Yersinia enterocolitica, a causative agent of gastroenteritis. Yersinia enterocolitica is a significant food or waterborne enteric pathogen being … Read more

Tryptic Soy Agar

Tryptic Soy Agar

Tryptic Soy Agar is used as a general growth medium for the isolation and cultivation of microorganisms. They are general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow. This medium is also recommended … Read more