Biuret Test for Protein

Biuret Test for Protein

What does Biuret test for? Proteins are the complex compound formed by thousands of amino acids. Amino acids are amphoteric electrolytes having carboxyl and amino groups that act like acid and base. It has one positive charge and one negative … Read more

Carbohydrate Fermentation Test | Sugar Fermentation Test

Carbohydrate fermentation test

Introduction Carbohydrates are organic molecules that comprise carbon, hydrogen, and oxygen in the ratio (CH2O)n. There are three types of carbohydrates based on their structure and number of carbon atoms present, they are monosaccharides (simple sugar containing 3-7 carbon atoms), … Read more

Oxidase Test- Principle, Procedure and Results

Oxidase Test

Objective of Oxidase Test To determine the ability of the organism to produce the cytochrome oxidase enzyme. Principle of Oxidase Test The oxidase test is designed for specifically detecting the presence of the terminal enzyme system in aerobic respiration called cytochrome … Read more

Optochin Susceptibility Test- Principle, Procedure and Results

Optochin Susceptibility Test- Principle, Procedure and Results

Objectives of Optochin Susceptibility Test To determine the effect of Optochin (ethyl hydrocupreine hydrochloride) on an organism. To differentiate between alpha-hemolytic Streptococcus pneumoniae from other alpha-hemolytic viridans Streptococci. Principle of Optochin Susceptibility Test Optochin (ethylhydrocupreine hydrochloride) is a chemical and is completely soluble in … Read more

Novobiocin Susceptibility Test- Principle, Procedure, Results, Uses

Result Interpretation of Novobiocin Susceptibility Test

Objectives of Novobiocin Susceptibility Test To determine the susceptibility pattern of a bacterium to novobiocin. To distinguish or differentiate Staphylococcus saprophyticus from other coagulase-negative staphylococci. Principle of Novobiocin Susceptibility Test Novobiocin is an antibiotic interfering with the unpackaging and repackaging of DNA during DNA … Read more

Methyl Red (MR) Test- Principle, Procedure, Results, Uses

Result Interpretation of Methyl Red (MR) Test

Objectives of Methyl Red (MR) Test To differentiate two major types of facultative anaerobic enteric bacteria based on the production of acid. It identifies bacterial ability to produce stable acid end products by means of a mixed-acid fermentation of glucose. Principle of Methyl … Read more