22 Types of Spectroscopy with Definition, Principle, Steps, Uses

Types of Spectroscopy

What is spectroscopy? Spectroscopy is the study of the interaction between light and matter where the absorption and emission of light or other radiation by the matter are studied and measured. Spectroscopy mainly deals with the dispersion of light and other radiations that is caused by an object which allows the study of various properties of the object. The measurement …

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Ultracentrifuge- Definition, Principle, Types, Parts, Procedure, Uses

Ultracentrifuge

Ultracentrifuge Definition Ultracentrifuge is a sophisticated and advanced centrifuge that operates at an extremely high speed and separates smaller molecules that cannot be separated from the traditional centrifuges. The speed of the rotors in ultracentrifuge can range from 60,000 rpm to 150,000 rpm. Ultracentrifuges are mostly operated in more facilitated laboratories to perform more advanced operations. These are larger in …

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Laminar flow hood/cabinet- definition, parts, principle, types, uses

Laminar flow hood or cabinet

Definition A Laminar flow hood/cabinet is an enclosed workstation that is used to create a contamination-free work environment through filters to capture all the particles entering the cabinet. These cabinets are designed to protect the work from the environment and are most useful for the aseptic distribution of specific media and plate pouring. Laminar flow cabinets are similar to biosafety …

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14 Types of Chromatography (Definition, Principle, Steps, Uses)

Types of Chromatography (Definition, Principle, Steps, Uses)

Chromatography Definition Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. A wide range of chromatographic procedures makes use of differences in size, binding affinities, charge, and other properties to separate materials. It is a powerful separation tool that is used in all branches of …

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Types of Centrifuge & Centrifugation (definition, principle, uses)

Types of Centrifuge and Centrifugation

Centrifuge definition A centrifuge is a device used to separate components of a mixture on the basis of their size, density, the viscosity of the medium, and the rotor speed. The centrifuge is commonly used in laboratories for the separation of biological molecules from a crude extract. In a centrifuge, the sample is kept in a rotor that is rotated …

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Autoclave- definition, parts, principle, procedure, types, uses

Autoclave- definition, parts, principle, procedure, types, uses

Autoclave Definition An autoclave is a machine that provides a physical method of sterilization by killing bacteria, viruses, and even spores present in the material put inside of the vessel using steam under pressure. Autoclave sterilizes the materials by heating them up to a particular temperature for a specific period of time. The autoclave is also called a steam sterilizer …

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Flow Cytometry-Definition, Principle, Parts, Steps, Types, Uses

Flow Cytometry-Definition, Principle, Parts, Steps, Types, Uses

Flow Cytometry Definition Flow cytometry is a standard laser-based technology that is used in the detection and measurement of physical and chemical characteristics of cells or particles in a heterogeneous fluid mixture. The use of flow cytometry has increased over the years as it provides a rapid analysis of multiple characteristics (both qualitative and quantitative) of the cells. The properties …

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Instruments used in Microbiology Lab with Principle and Uses

Instruments used in Microbiology Lab with Principle and Uses

The instruments used in the microbiology labs include a bunch of different kinds of instruments required for a lot of different processes conducted within those laboratories. Figure: Instruments used in Microbiology Lab with Principle and Uses. Image created with biorender.com 1. Analytical Balance An analytical balance is a type of balance that is commonly used for the measurement of mass …

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Gel Filtration Chromatography

Gel Filtration Chromatography

Gel Filtration Chromatography Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc. Gel filtration is a technique in which the separation of components is based on the difference in molecular weight or size. It is the simplest and mildest of all the chromatography techniques and …

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Gel Permeation Chromatography

Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography is also called as gel filtration or size exclusion chromatography. In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc. The separation is based on the analyte molecular sizes since the gel behaves like a molecular sieve. This technique …

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