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Instrumentation

Instrumentation is a collection of devices and mechanics used to measure, evaluate, and test systems.

  • In biology, the instrumentation is considered a part of biomedical engineering where the working mechanism and the parts of a system are studies.
  • Instrumentation in biology or Bioinstrumentation is primarily concentrated on bringing the engineering and medical worlds together.
  • Different devices like centrifuges and autoclaves that are common in biological laboratories are a part of the instrumentation. Studies done in instrumentations allow the understanding of the parts and working mechanisms of such devices.
  • Other important techniques include chromatography which is routinely performed in laboratories to separate biological systems, which are also equally important.
  • Instrumentation is still an emerging discipline with new pieces of machinery still being produced for convenience in laboratories.
  • The application of bio-instruments is not only limited to laboratories as modern agriculture makes use of bio-instruments to monitor and evaluate soil and to measure plant growth.
  • Besides, these instruments can also be used to determine the physiological activities of plants like the carbon dioxide trade rate, net photosynthesis, etc.
  • Processes like electrophoresis make use of nucleic acids along with biosensors to compare the DNA sequences. This enables genetic testing and diagnosis of various diseases.
  • Genetic testing is heavily influenced by the progress made in instrumentation as new devices and techniques are discovered which makes the process faster, more accurate, and more reliable.
  • As RT-PCR and blotting are considered advanced molecular diagnosis methods, disease diagnosis becomes faster and more accurate than ever. 
  • Determination of radioisotopes and concentration of a solution has been made easier with techniques like spectroscopy and colorimetry.
  • As the discipline continues to grow, the main focus of the field remains in the improvements of technology to make the medical processes faster and more efficient.
  • Instrumentation, thus, is slowly becoming an integral part of all biological fields with new emerging new technology and the discovery of fast and reliable procedures.

Gas Chromatography-Mass Spectrometry (GC-MS)

April 7, 2026April 5, 2026 by Sanju Tamang
Gas chromatography-mass spectrometry (GC-MS)

Gas chromatography-mass spectrometry (GC-MS) is a method that combines the separation ability of gas chromatography (GC) with the detection ability of mass spectrometry (MS) for the qualitative and quantitative analysis … Read more

Gas Liquid Chromatography: Principle, Parts, Steps, Uses

April 5, 2026April 4, 2026 by Sanju Tamang
Gas-Liquid Chromatography

Gas Liquid chromatography (GLC) is a type of gas chromatography (GC) that uses a mobile gas phase and a stationary liquid phase to separate and analyze volatile compounds in a … Read more

Gel Permeation Chromatography: Principle, Parts, Steps, Uses

April 5, 2026April 3, 2026 by Sanju Tamang
Gel Permeation Chromatography

Gel Permeation Chromatography (GPC) is a liquid chromatography method used to separate and analyze molecules based on their hydrodynamic volume or size in solution. It is used to determine the … Read more

Hydrodynamic Chromatography (HDC): Principle, Steps, Uses

April 5, 2026April 2, 2026 by Sanju Tamang
Hydrodynamic Chromatography (HDC)

Hydrodynamic chromatography (HDC) is a liquid chromatography method used to separate particles in solution based on their size under laminar flow conditions. Separation occurs according to how particles of different … Read more

HPLC: Principle, Instrumentation, Steps, Types, Uses, Diagram

April 5, 2026April 1, 2026 by Sanju Tamang
High Performance Liquid Chromatography (HPLC)

High-performance liquid chromatography (HPLC) is a liquid chromatography method that is used to separate and analyze components in liquid mixtures. It is a modified form of column chromatography that uses … Read more

Gas Chromatography: Principle, Parts, Steps, Procedure, Uses

March 13, 2026March 8, 2026 by Sanju Tamang
Principle of Gas Chromatography

Gas chromatography (GC) is a type of chromatography used to separate and analyze volatile compounds. This separation occurs in an instrument called gas chromatograph that uses an inert gas as … Read more

Flash Chromatography: Principle, Parts, Steps, Examples, Uses

March 13, 2026March 7, 2026 by Sanju Tamang
Flash Chromatography

Flash chromatography is a method used for the rapid separation and purification of chemical compounds in a mixture. It is also known as flash column chromatography or medium-pressure liquid chromatography. … Read more

Fast Protein Liquid Chromatography (FPLC): Principle, Steps, Uses

March 11, 2026March 6, 2026 by Sanju Tamang
Fast Protein Liquid Chromatography (FPLC)

Fast Protein Liquid Chromatography (FPLC) is a liquid chromatography technique used for the separation and purification of proteins and other biomolecules. It was developed in the 1980s by Pharmacia and … Read more

Dye Ligand Chromatography: Principle, Components, Steps, Uses

March 10, 2026March 5, 2026 by Sanju Tamang
Dye Ligand Chromatography

Dye ligand chromatography is a type of affinity chromatography that uses synthetic dyes as ligands to purify target biomolecules, such as proteins and enzymes, from complex mixtures.  Unlike traditional affinity … Read more

Column Chromatography: Principle, Parts, Types, Steps, Uses

March 1, 2026 by Sanju Tamang
Column Chromatography

Column chromatography is a method that separates and purifies the components of a mixture on a vertical column or tube based on their interactions with the stationary and mobile phases. … Read more

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