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Microscopy

Microscopy is the study of devices (microscopes) that are used to view objects or certain areas that cannot be seen with a naked eye.

  • Microscopes work on the physical principle of magnification where the image of an object is magnified so that it can be visible.
  • The substances that can only be seen with a microscope are called microscopic substances.
  • Microscopes are imperative in areas like microbiology that deals with the structure and function of microscopic living beings.
  • Microscopy is further divided into three branches; optical microscopy, electron microscopy, and X-ray microscopy.
  • X-ray microscopy is a fairly new technology that is responsible for detailed imaging of subcellular organelles like the nucleus and chromosomes.
  • Microscopy, importantly optical microscopy, began with the discovery of the first microscope by Anton Von Leeuwenhoek.
  •  The complexity of microscopy since then has increased rapidly with new and advanced microscopes with higher magnification and resolution.
  • In an optical microscope, the rays of light are passed through a series of glass lenses to produce a magnified image on the observer’s eyes. Compound microscopes are the most common type of microscope, mostly used for research and teaching purposes.
  • In the case of an electron and X-ray microscope, an electron beam is created which produced a digital magnified image of an object.
  • Electron microscopes have very high magnification and resolution which produces clear enlarged images of objects as small as an atom.
  • Depending on the nature of the sample, different types of microscopes, including bright field microscope, fluorescence microscope, phase contrast, and darkfield microscopes, are also available.
  • The magnification of these microscopes depends on the type of lens used in the system which produces images of different magnitude and resolution so that they can be viewed.
  • Microscopy is important in different areas of science like histology, cytology, and bacteriology. Microscopic examination of the morphology and structure of cells has been used as an essential technique for the identification of microorganisms.

Multiphoton Microscopy: Principle, Parts, Steps, Uses

March 24, 2025March 23, 2025 by Dipika Shrestha
Multiphoton Microscopy

Historically, significant milestones in the evolution of life science and technology have led to advancements in the development of microscopy techniques. From visualization of microbial cells to studying subcellular components, … Read more

Parts of a Microscope with Functions and Labeled Diagram

April 9, 2025March 19, 2025 by Prashant Dahal
Microscope Parts

Having been constructed in the 16th Century, microscopes have revolutionized science with their ability to magnify small objects such as microbial cells, producing images with definitive structures that are identifiable … Read more

Microscope Diaphragm: Types, Functions & Adjustment Tips

April 9, 2025March 5, 2025 by Krisha Karki
Diaphragm of a Microscope

Microscopes are critical tools in various fields, including biology, medicine, chemistry, geology, and materials science, enabling researchers, scientists, and students to explore microscopic specimens’ intricate structures and features. The diaphragm … Read more

Confocal Laser Scanning Microscope: Principle, Parts, Uses

December 20, 2024 by Dipika Shrestha
Confocal Laser Scanning Microscope

In 1952, Marvin Lee Minsky, an American computer scientist, patented confocal imaging based on the idea of producing a point source of light and rejecting out-of-focus light using illumination. Confocal … Read more

Epi-Fluorescence Microscopy: Principle, Parts, Steps, Uses

December 19, 2024 by Dipika Shrestha
Epi-Fluorescence Microscopy

The energy emitted from certain materials, when irradiated with a specific wavelength of light, can be detected as visible light due to energy reaction. In microscopy, the phenomenon of fluorescence, … Read more

Differential Interference Contrast (DIC) Microscope

December 18, 2024December 17, 2024 by Dipika Shrestha
Differential Interference Contrast (DIC) Microscope

Differential Interference Contrast (DIC) Microscope is widely used to image unstained and transparent living specimens and observe the structure and motion of isolated organelles, making it an alternative to conventional … Read more

27 Amazing Things Under the Microscope with Diagrams

January 2, 2025December 2, 2024 by Anupama Sapkota
How these 26 things look like under the microscope

1. Amoeba under the microscope Direct observation Observation after staining 2. Algae under the microscope Based on their morphology, algae are divided into separate groups: Chlorophyta Chromophyta Cryptophyta Rhodophyta Dinoflagellata … Read more

Brightfield Microscope: Principle, Parts, Applications

September 4, 2024August 23, 2024 by Faith Mokobi
Brightfield Microscope

Brightfield Microscope is an optical microscope that uses light rays to produce a dark image against a bright background. It is the standard microscope that is used in Biology, Cellular … Read more

Electron Microscope: Principle, Types, Uses, Labeled Diagram

May 26, 2024May 17, 2024 by Sagar Aryal
Electron Microscope

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. It is a special type of microscope having a high resolution of … Read more

Scanning Electron Microscope (SEM): Principle, Parts, Uses

May 26, 2024May 5, 2024 by Faith Mokobi
Scanning Electron Microscope (SEM)

The first Scanning Electron Microscope was initially made by Mafred von Ardenne in 1937 with an aim to surpass the transmission electron Microscope. He used high-resolution power to scan a … Read more

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