Twins- Types, Causes and Significance

Twins

Twins- Types, Causes and Significance Normally, a woman gives birth to only one young at a time, but sometimes more than one child are born to a woman at the same time. The delivery of more than one baby by a mother is termed as multiple births. Most commonly in the case of multiple births, … Read moreTwins- Types, Causes and Significance

Eukaryotic DNA Replication- Features, Enzymes, Process, Significance

DNA Replication

Eukaryotic DNA Replication- Features, Enzymes, Process, Significance DNA replication is the process by which an organism duplicates its DNA into another copy that is passed on to daughter cells. Replication occurs before a cell divides to ensure that both cells receive an exact copy of the parent’s genetic material. Features of Eukaryotic DNA Replication Replication is … Read moreEukaryotic DNA Replication- Features, Enzymes, Process, Significance

Restriction Fragment Length Polymorphism (RFLP)

Steps Involved in Restriction Fragment Length Polymorphism (RFLP)

Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. It is a technique that exploits variations in homologous DNA sequences. A restriction fragment length polymorphism is defined by the existence of alternative alleles associated with … Read moreRestriction Fragment Length Polymorphism (RFLP)

Types of Mutations

Types of Mutations

Types of Mutations Mutation is a process that produces a gene or chromosome that differs from the wild type (arbitrary standard for what “normal” is for an organism). The mutation may result due to changes either on the gene or the chromosome itself. Thus, broadly mutation maybe: Gene mutation where the allele of a gene … Read moreTypes of Mutations

Gene Expression

Gene Expression

Gene Expression Gene expression is the process by which the genetic code – the nucleotide sequence – of a gene is used in the synthesis of a functional gene product. It refers to a complex series of processes in which the information encoded in a gene is used to produce a functional product such as a protein … Read moreGene Expression

Agarose Gel Electrophoresis

Agarose Gel Electrophoresis

Agarose Gel Electrophoresis Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA , RNA or proteins in a matrix of agarose. Agarose is a natural linear polymer extracted from seaweed that forms a gel matrix by hydrogen-bonding when heated in a buffer and allowed to … Read moreAgarose Gel Electrophoresis

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes The genome of an organism encompasses all of the genes of that organism. Gene is a distinct sequence of nucleotides forming part of a chromosome, the order of which determines the order of monomers in a polypeptide or nucleic acid molecule. Thus a protein-coding gene is defined as a region of … Read moreProkaryotic and Eukaryotic Chromosomes

Pulsed Field Gel Electrophoresis (PFGE)

The Method of Pulsed Field Gel Electrophoresis (PFGE)

Pulsed Field Gel Electrophoresis (PFGE) Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction. As DNA larger than 15-20kb migrating through a gel essentially moves together in a size-independent manner, the standard gel … Read morePulsed Field Gel Electrophoresis (PFGE)

Mendel’s Experiment and Laws

Mendel's Experiment

Mendel’s Experiment and Laws In the 1860’s, an Austrian monk named Gregor Mendel introduced a new theory of inheritance based on his experimental work with pea plants. Mendel believed that heredity is the result of discrete units of inheritance, and every single unit (or gene) was independent in its actions in an individual’s genome. According to … Read moreMendel’s Experiment and Laws

Recombinant DNA Technology- Steps, Applications and Limitations

Recombinant DNA technology

Recombinant DNA Technology- Steps, Applications and Limitations Recombinant DNA technology refers to the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA (rDNA), on the other hand is the general name for a piece of DNA that … Read moreRecombinant DNA Technology- Steps, Applications and Limitations