Negative Staining- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation Objectives of Negative Staining To perform a negative staining procedure. To understand the benefit obtained from visualizing unstained microorganisms. Principle of Negative Staining Negative staining requires the use of an acidic stain such as India ink or nigrosin. The acidic stain, with its negatively charged chromogen, will not … Read more Negative Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation Objectives of Simple Staining To perform a simple staining procedure. To compare the morphological shapes and arrangements of bacterial cells. Principle of Simple Staining In simple staining, the bacterial smear is stained with a single reagent, which produces a distinctive contrast between the organism and its background. Basic stains with … Read more Simple Staining- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a … Read more Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. … Read more Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells … Read more Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation