Streptococcus Selective Agar

Streptococcus Selective Agar

Streptococcus Selective Agar Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness of hemolytic zones produced by these organisms. Streptococcus Selective Agar is a selective medium recommended for use in the primary isolation of … Read more Streptococcus Selective Agar

Differences between Staphylococcus and Streptococcus

Staphylococcus and Streptococcus Differences

Differences between Staphylococcus and Streptococcus Staphylococcus and Streptococcus are Gram positive and the two most common pathogenic cocci of medical importance. They are both non-motile, non-sporing and facultative anaerobes which can be differentiated on following grounds: S.N. Character Staphylococcus Streptococcus 1.       Cellular Arrangement Spherical cells in clusters (grape like clusters). Spherical or ovoid cells in chains or pairs. 2.       … Read more Differences between Staphylococcus and Streptococcus

Biochemical Test of Streptococcus canis

Biochemical Test of Streptococcus canis Basic Characteristics Properties (Streptococcus canis) CAMP Negative (-ve) Catalase Negative (-ve) Gram Staining Positive (+ve) OF (Oxidative-Fermentative) Facultative anaerobes Shape Cocci Urease Negative (-ve) VP (Voges Proskauer) Negative (-ve) Fermentation of Adonitol Negative (-ve) Arabinose Negative (-ve) Arabitol Negative (-ve) Arbutin Positive (+ve) Dulcitol Negative (-ve) Erythritol Negative (-ve) Fructose … Read more Biochemical Test of Streptococcus canis

Biochemical Test of Streptococcus mutans

Biochemical Test of Streptococcus mutans

Biochemical Test of Streptococcus mutans Basic Characteristics Properties (Streptococcus mutans) Capsule Capsulated Catalase Negative (-ve) Gram Staining Positive (+ve) Hemolysis Alfa Hemolysis Motility Non-motile OF (Oxidative-Fermentative) Facultative anaerobes Oxidase Negative (-ve) Shape Cocci Spore Non-sporing Urease Negative (-ve) VP (Voges Proskauer) Positive (+ve) Fermentation of Adonitol Negative (-ve) Arabinose Negative (-ve) Arbutin Positive (+ve) Cellobiose … Read more Biochemical Test of Streptococcus mutans

Biochemical Test of Streptococcus agalactiae

Biochemical Test of Streptococcus agalactiae

Biochemical Test of Streptococcus agalactiae Basic Characteristics Properties (Streptococcus agalactiae) CAMP Positive (+ve) Capsule Positive (+ve) Catalase Negative (-ve) Coagulase Negative (-ve) Flagella Non-flagellated Gram Staining Positive (+ve) Hemolysis Beta Hemolysis Motility Non-motile OF (Oxidative-Fermentative) Facultative anaerobes Oxidase Negative (-ve) PYR Negative (-ve) Shape Cocci Spore Non-sporing Urease Negative (-ve) VP (Voges Proskauer) Variable Fermentation … Read more Biochemical Test of Streptococcus agalactiae

Habitat and Morphology of Streptococcus pneumoniae

Habitat and Morphology of Streptococcus pneumoniae

Habitat of Streptococcus pneumoniae Streptococcus pneumoniae are found worldwide. Found in primates, livestock and felines. They are the part of normal flora of upper respiratory tract infection in humans. Mostly found in throat and nasal passage. They infection mostly children in winter seasons. They are Mesophilic, 30 to 35°C. Morphology of Streptococcus pneumoniae They are … Read more Habitat and Morphology of Streptococcus pneumoniae

Biochemical Test of Streptococcus pyogenes

Biochemical Test of Streptococcus pyogenes

Biochemical Test of Streptococcus pyogenes Characteristics Properties (Streptococcus pyogenes) CAMP Negative (-ve) Capsule Capsulated Catalase Negative (-ve) Gram Staining Positive (+ve) Hemolysis Beta Motility Non-Motile OF (Oxidative-Fermentative) Fac. Anaerobes PYR Positive (+ve) Shape Cocci Spore Non-Sporing Urease Negative (-ve) VP (Voges Proskauer) Negative (-ve) 6.5% NaCl Growth Negative (-ve) Fermentation of Adonitol Negative (-ve) Arabinose … Read more Biochemical Test of Streptococcus pyogenes

Biochemical Test of Streptococcus pneumoniae

Biochemical Test of Streptococcus pneumoniae

Biochemical Test of Streptococcus pneumoniae Basic Characteristics Properties (Streptococcus pneumoniae) Bile Solubility Positive (+ve) Catalase Negative (-ve) Gram Staining Positive (+ve) Hemolysis Alfa Hemolysis Motility Non-motile OF (Oxidative-Fermentative) Facultative anaerobes Oxidase Negative (-ve) Shape Diplococci Spore Non-sporing Urease Negative (-ve) VP (Voges Proskauer) Negative (-ve) Fermentation of Arabinose Positive (+ve) Arbutin Negative (-ve) Dulcitol Negative … Read more Biochemical Test of Streptococcus pneumoniae

Laboratory diagnosis, Treatment and Prevention of Streptococcus pneumoniae

Laboratory diagnosis, Treatment and Prevention of Streptococcus pneumoniae

Laboratory diagnosis of Streptococcus pneumoniae Specimen: Sputum, blood, endotracheal aspirate, bronchoalveolar lavage, cerebrospinal fluid (CSF), pleural fluid, joint fluid, abscess fluid, bones, and other biopsy material. Microscopy Gram staining of sputum shows lancet shaped Gram-positive cocci in pairs. Fresh emulsified sputum mixed with antiserum causes capsule swelling (the quellung reaction) for identification of pneumococci. In … Read more Laboratory diagnosis, Treatment and Prevention of Streptococcus pneumoniae

Virulence factors, Pathogenesis and Clinical manifestations of Streptococcus pneumoniae

Virulence factors, Pathogenesis and Clinical manifestations of Streptococcus pneumoniae

Virulence factors of Streptococcus pneumoniae Polysaccharide capsule The capsule is antiphagocytic, inhibiting complement deposition and phagocytosis. 2. Cell wall associated polymers and proteins Teichoic acid – binds to epithelial cells and activates alternative complement pathway Protein adhesion – binds to epithelial cells Peptidogylcan – activates alternative complement pathway Phosphorylcholine – mediates invasion of host cell … Read more Virulence factors, Pathogenesis and Clinical manifestations of Streptococcus pneumoniae

Laboratory diagnosis of Streptococcus pyogenes

Laboratory diagnosis of Streptococcus pyogenes

Laboratory diagnosis of Streptococcus pyogenes 1. Specimens Throat swab Pus Cerebrospinal fluid Blood Serum for antibody determinant 2. Smear Gram staining Purple color cocci in chain arrangement Not to be confused with Viridans Streptococci from throat swab sample since both have same appreance 3. Culture Culture on blood agar Addition of bacitracin in inoculum: S … Read more Laboratory diagnosis of Streptococcus pyogenes

Habitat and Morphology of Streptococcus pyogenes

Habitat and Morphology of Streptococcus pyogenes

Habitat and Morphology of Streptococcus pyogenes Habitat of Streptococcus pyogenes They are found in throat and skin of human. They are commensals of upper respiratory tract. Carrier rate of S. pyogenes in the respiratory tract is only few in adults, over 10% in children attending kindergarten. Opportunistic pathogens May survive in dust for some times. Morphology … Read more Habitat and Morphology of Streptococcus pyogenes

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are … Read more Bile Esculin Test- Principle, Procedure and Result Interpretation