Enterotoxigenic E. coli (ETEC)

Enterotoxigenic E. coli (ETEC)

Enterotoxigenic E. coli (ETEC) is defined as containing the E. coli strains that elaborate at least one member of two defined groups of enterotoxins: heat-stable toxin (ST) and heat-stable toxin (LT). Enterotoxigenic Escherichia coli, or ETEC, is thus the name given to a group … Read more

Enteroinvasive E. coli (EIEC)

Enteroinvasive E. coli (EIEC)

Enteroinvasive E. coli (EIEC) strains are rare in both developed and developing countries. EIEC infections are characterized by a period of watery diarrhea that precedes the onset of scanty dysenteric stools containing blood and mucus. Pathogenic strains are primarily associated … Read more

CHROMagar (Rambach Agar)

CHROMagar (Rambach Agar)

The first chromogenic culture medium for detection of E. coli was invented and patented by Dr. A. Rambach in 1979. This technology is color-based differentiation method. It is based on soluble colorless molecules (called chromogens), composed of a substrate (targeting … Read more

Enteroaggregative E. coli (EAEC)

Stages of pathogenesis of EAEC

Enteroaggregative E.  coli (EAEC) are a heterogeneous collection of strains characterized by their autoagglutination in a “stacked-brick” arrangement over the epithelium of the small intestine and, in some cases, the colon. EAEC strains are currently defined as  E. coli strains that do … Read more

E. coli Pathotypes- ETEC, EPEC, EHEC, EAEC, EIEC, DAEC

E. coli Pathotypes

Escherichia coli (commonly abbreviated E. coli) is a Gram-negative, facultative anaerobic, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Human Escherichia coli strains are classified as commensal microbiota E. coli, enterovirulent E. coli, and extraintestinal pathogenic E. coli (ExPEC) on … Read more

Rapid Method for Enumeration of E. coli Biotype I

Rapid Method for Enumeration of E. coli Biotype I

This method is modified by Anderson and Parker. Prepare serial tenfold dilutions in peptone water of food. Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar. Transfer duplicate 1.0 mL aliquots of each dilution to cellulose … Read more