Biochemical Test of Escherichia coli (E. coli)

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Escherichia coli (E. coli)

Basic CharacteristicsProperties (E. coli)
CapsuleCapsulated
CatalasePositive (+ve)
CitrateNegative (-ve)
CoagulaseNegative (-ve)
FlagellaFlagellated
GasPositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingNegative (-ve)
Growth in KCNNegative (-ve)
H2SNegative (-ve)
HemolysisNegative (-ve)
IndolePositive (+ve)
MotilityMotile
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Fermentative
OxidaseNegative (-ve)
PigmentNegative (-ve)
PYRNegative (-ve)
ShapeRod
SporeNon-Sporing
TSIA (Triple Sugar Iron Agar)Acid/Acid, Gas +ve
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)

Fermentation of

AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DNaseNegative (-ve)
DulcitolVariable
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolVariable
LactosePositive (+ve)
MalonateNegative (-ve)
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibiosePositive (+ve)
MucatePositive (+ve)
Myo-InositolNegative (-ve)
RaffinoseVariable
RhamnosePositive (+ve)
SalicinVariable
SorbitolPositive (+ve)
SucroseVariable
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)

Enzymatic Reactions

Acetate UtilizationPositive (+ve)
Aesculine HydrolysisVariable
Arginine DihydrolaseNegative (-ve)
Beta LactamasePositive (+ve)
Esculin HydrolysisVariable
LipaseNegative (-ve)
Lysine DecarboxylasePositive (+ve)
ONPG (β-galactosidase)Positive (+ve)
Ornithine DecarboxylaseVariable
Phenylalanine DeaminaseNegative (-ve)

Escherichia coli (E. coli) Gram Stain

Figure: Gram Stain of E. coli. Source: Art.com

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