Chromatography- definition, principle, types, applications

Chromatography- Principle, Types and Applications

What is chromatography? Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. The Russian botanist Mikhail Tswett coined the term chromatography in 1906. The first … Read more

Gel Filtration Chromatography

Gel Filtration Chromatography

Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc. Gel filtration is a technique in which the separation of components is based on the difference … Read more

Gel Permeation Chromatography

Gel Permeation Chromatography

Gel permeation chromatography is also called as gel filtration or size exclusion chromatography. In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc. The separation … Read more

Ion Exchange Chromatography

Ion Exchange Chromatography

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their … Read more

Column Chromatography

Column Chromatography

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It is a separation technique in which a mobile phase carrying a mixture is caused to … Read more

Affinity Chromatography

Affinity Chromatography

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It is a separation technique in which a mobile phase carrying a mixture is caused to … Read more

Paper Chromatography

Paper Chromatography

Paper chromatography (PC) is a type of a planar chromatography whereby chromatography procedures are run on a specialized paper. PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation, … Read more

Thin Layer Chromatography

Procedure of Thin Layer Chromatography (TLC)

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. In this physical method of separation, the components to be separated are distributed between two phases, … Read more

High-Performance Liquid Chromatography (HPLC)

High Performance Liquid Chromatography (HPLC)

High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified … Read more