14 Types of Chromatography (Definition, Principle, Steps, Uses)

Types of Chromatography (Definition, Principle, Steps, Uses)

Chromatography Definition Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. A wide range of chromatographic procedures makes use of differences in size, binding affinities, charge, and other properties to separate materials. It is a powerful separation tool that is used in all branches of …

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Gel Filtration Chromatography

Gel Filtration Chromatography

Gel Filtration Chromatography Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc. Gel filtration is a technique in which the separation of components is based on the difference in molecular weight or size. It is the simplest and mildest of all the chromatography techniques and …

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Gel Permeation Chromatography

Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography is also called as gel filtration or size exclusion chromatography. In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc. The separation is based on the analyte molecular sizes since the gel behaves like a molecular sieve. This technique …

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Ion Exchange Chromatography

Ion Exchange Chromatography

Ion Exchange Chromatography Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to ion exchangers. The principle of separation is thus by reversible exchange of ions between the target …

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Column Chromatography

Column Chromatography

Column Chromatography Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It is a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. There are a number of different kinds of chromatography, …

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Affinity Chromatography

Affinity Chromatography

Affinity Chromatography Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It is a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. Affinity chromatography is a type of liquid chromatography for …

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Gas chromatography- definition, principle, working, uses

Principle of Gas Chromatography

What is gas chromatography? Gas chromatography differs from other forms of chromatography in that the mobile phase is a gas and the components are separated as vapors. It is thus used to separate and detect small molecular weight compounds in the gas phase. The sample is either a gas or a liquid that is vaporized in the injection port. The …

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Paper Chromatography

Paper Chromatography

Paper Chromatography Paper chromatography (PC) is a type of a planar chromatography whereby chromatography procedures are run on a specialized paper. PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation, identification and quantitative determination of organic and inorganic compounds. It was first introduced by German scientist Christian Friedrich …

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Thin Layer Chromatography

Procedure of Thin Layer Chromatography (TLC)

Thin Layer Chromatography Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. In this physical method of separation, the components to be separated are distributed between two phases, one of which is stationary (stationary phase) while the other (the mobile phase) moves in a definite …

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High-Performance Liquid Chromatography (HPLC)

High Performance Liquid Chromatography (HPLC)

High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy.  In the 1960s the column chromatography LC with its low-pressure suitable glass columns was further developed to the …

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