Cetrimide Agar Test- Principle, Procedure and Result Interpretation

Cetrimide Agar Test- Principle, Procedure and Result Interpretation

Cetrimide Agar Test- Principle, Procedure and Result Interpretation Objective of Cetrimide Agar Test This test is primarily used to isolate and purify Pseudomonas aeruginosa from contaminated specimens. Principle of Cetrimide Agar Test The test is used to determine the ability of an organism to grow in the presence of cetrimide, a toxic substance that inhibits the growth … Read moreCetrimide Agar Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation Objective of Catalase Test This test differentiates catalase-positive micrococcal and staphylococcal species from catalase-negative streptococcal species. Principle of Catalase Test Aerobic and facultative anaerobic organisms produce two toxins during normal metabolism, hydrogen peroxide (H2O2) and superoxide radical (O2−). These bacteria have two enzymes that detoxify the products of normal metabolism. … Read moreCatalase Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation Objective of CAMP Test The Christie, Atkins, and Munch-Peterson (CAMP) test is used to differentiate group B streptococci (Streptococcus agalactiae–positive) from other streptococcal species. Listeria monocytogenes also produces a positive CAMP reaction. Principle of CAMP Test Certain organisms (including group B streptococci) produce a diffusible extracellular hemolytic protein … Read moreCAMP Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation Purpose of Butyrate Disk Test This is a rapid test to detect the enzyme butyrate esterase, to aid identification of Moraxella (Branhamella) catarrhalis. Principle of Butyrate Disk Test Organisms capable of producing butyrate esterase hydrolyze bromochlorindolyl butyrate. Hydrolysis of the substrate in the presence of butyrate esterase releases indoxyl, … Read moreButyrate Disk Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation Objective of Bile Solubility Test This test differentiates Streptococcus pneumoniae (positive–soluble) from alpha-hemolytic streptococci (negative–insoluble). Principle of Bile Solubility Test Bile or a solution of a bile salt (e.g., sodium desoxycholate) rapidly lyses pneumococcal colonies. Lysis depends on the presence of an intracellular autolytic enzyme, amidase. Bile salts lower … Read moreBile Solubility Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are … Read moreBile Esculin Test- Principle, Procedure and Result Interpretation

Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation Objectives of Bacitracin Susceptibility Test This test is used for presumptive identification and differentiation of beta-hemolytic group A streptococci (Streptococcus pyogenes– susceptible) from other beta-hemolytic streptococci. It is also used to distinguish staphylococci species (resistant) from micrococci (susceptible). Principle of Bacitracin Susceptibility Test The antibiotic bacitracin inhibits the … Read moreBacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation Objective of Acetate Utilization Test To differentiate organisms based on ability to use acetate as the sole source of carbon. Generally used to differentiate Shigella sp. from Escherichia coli. Principle of Acetate Utilization Test This test is used to differentiate an organism capable of using acetate as the sole source … Read moreAcetate Utilization Test- Principle, Procedure and Result Interpretation

Acetamide Utilization Test- Principle, Procedure and Result Interpretation

Acetamide Utilization Test- Principle, Procedure and Expected Results

Acetamide Utilization Test- Principle, Procedure and Result Interpretation Objective of Acetamide Utilization Test To differentiate microorganisms based on the ability to use acetamide as the sole source of carbon. Principle of Acetamide Utilization Test Bacteria capable of growth on this medium produce the enzyme acylamidase, which deaminates acetamide to release ammonia. The production of ammonia results in … Read moreAcetamide Utilization Test- Principle, Procedure and Result Interpretation