Biochemical Test of Mycobacterium tuberculosis

Biochemical Test of Mycobacterium tuberculosis

Basic CharacteristicsProperties (Mycobacterium tuberculosis)
5 % NaCl ToleranceNegative (-ve)
68°C Catalase TestNegative (-ve)
Acid Fast StainPositive (+ve)
Acid PhosphataseNegative (-ve)
Amidase TestPositive (+ve)
Arylsulphatase TestNegative (-ve)
Growth at 22°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth at 42°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth on P-Nitrobenzoic AcidNegative (-ve)
Growth on TCH (10 mg/ml)Positive (+ve)
Iron UptakeNegative (-ve)
Neutral Red TestPositive (+ve)
Niacin TestPositive (+ve)
Nitrate Reduction TestPositive (+ve)
PigmentationNegative (-ve)
Peroxidase TestPositive (+ve)
Pyrazinamidase TestPositive (+ve)
Pyruvate stimulation growthNegative (-ve)
Semi Quantitative Catalase TestNegative (-ve)
Tellurite Reduction TestNegative (-ve)
Tween 80 HydrolysisNegative (-ve)
Urease TestPositive (+ve)

Mycobacterium tuberculosis gram-negative bacteria. Image plenoy m

Mycobacterium tuberculosis gram-negative bacteria. Image: plenoy m /

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

3 thoughts on “Biochemical Test of Mycobacterium tuberculosis”

  1. Great info, but M. tuberculosis is an acid resistant bacterium. The staining picturese shows that, coloring with carbofushin. In the information you have Gram negative. When M. tuberculosis is Gram stained, the reaction is Gram variable.

  2. Thanks for contribution plz my question is that staphylococcus bacteria is used to produce catalase enzymes this mean that the can be used in several aspects more especially in industry


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