Biochemical Test of Mycobacterium tuberculosis

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Biochemical Test of Mycobacterium tuberculosis

Basic CharacteristicsProperties (Mycobacterium tuberculosis)
5 % NaCl ToleranceNegative (-ve)
68°C Catalase TestNegative (-ve)
Acid Fast StainPositive (+ve)
Acid PhosphataseNegative (-ve)
Amidase TestPositive (+ve)
Arylsulphatase TestNegative (-ve)
Growth at 22°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth at 42°CNegative (-ve)
Growth at 25°CNegative (-ve)
Growth on P-Nitrobenzoic AcidNegative (-ve)
Growth on TCH (10 mg/ml)Positive (+ve)
Iron UptakeNegative (-ve)
Neutral Red TestPositive (+ve)
Niacin TestPositive (+ve)
Nitrate Reduction TestPositive (+ve)
PigmentationNegative (-ve)
Peroxidase TestPositive (+ve)
Pyrazinamidase TestPositive (+ve)
Pyruvate stimulation growthNegative (-ve)
Semi Quantitative Catalase TestNegative (-ve)
Tellurite Reduction TestNegative (-ve)
Tween 80 HydrolysisNegative (-ve)
Urease TestPositive (+ve)

Mycobacterium tuberculosis gram-negative bacteria. Image plenoy m

Mycobacterium tuberculosis gram-negative bacteria. Image: plenoy m /

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

3 thoughts on “Biochemical Test of Mycobacterium tuberculosis”

  1. Great info, but M. tuberculosis is an acid resistant bacterium. The staining picturese shows that, coloring with carbofushin. In the information you have Gram negative. When M. tuberculosis is Gram stained, the reaction is Gram variable.

  2. Thanks for contribution plz my question is that staphylococcus bacteria is used to produce catalase enzymes this mean that the can be used in several aspects more especially in industry


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