Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa

Basic Characteristics Properties (Pseudomonas aeruginosa)
Capsule Some Non-Capsulated and Some are encapsulated (Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate- Sumita Jain and Dennis E. Ohman 2005)
Catalase Positive (+ve)
Cetrimide Test Positive (+ve)
Citrate Positive (+ve)
Coagulase Negative (-ve)
Flagella Flagellated (Single)
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Negative (-ve)
H2S Negative (-ve)
Hemolysis Beta Hemolytic
Indole Negative (-ve)
Motility Motile (Unipolar)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Oxidative
Oxidase Positive (+ve)
Pigment Positive (+ve) (Blue/Green)
Shape Rod
Spore Non-Sporing
TSIA (Triple Sugar Iron Agar) Alkali/Alkali (Red/Red)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Hippurate Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Malonate Positive (+ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Negative (-ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

Pseudomonas aeruginosa Gram Stain

9 thoughts on “Biochemical Test of Pseudomonas aeruginosa”

  1. I did biochemical tests on several bacterial isolates and had different results. but after identifying the type of bacteria, the bacteria is Pseudomonas aeruginosa. Why do these bacteria have different biochemical tests even though they are one type of bacteria?

    Reply
    • Ya, there are some sources that say that they are encapsulated. So, we can say they are both capsulated and non-capsulated. They secrete a capsule-like polysaccharide called alginate according to Sumita Jain and Dennis E. Ohman 2005)

      Reply

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