Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa

Basic Characteristics Properties (Pseudomonas aeruginosa)
Capsule Some Non-Capsulated and Some are encapsulated (Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate- Sumita Jain and Dennis E. Ohman 2005)
Catalase Positive (+ve)
Cetrimide Test Positive (+ve)
Citrate Positive (+ve)
Coagulase Negative (-ve)
Flagella Flagellated (Single)
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Negative (-ve)
H2S Negative (-ve)
Hemolysis Beta Hemolytic
Indole Negative (-ve)
Motility Motile (Unipolar)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Oxidative
Oxidase Positive (+ve)
Pigment Positive (+ve) (Blue/Green)
Shape Rod
Spore Non-Sporing
TSIA (Triple Sugar Iron Agar) Alkali/Alkali (Red/Red)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Hippurate Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Malonate Positive (+ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Negative (-ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

Pseudomonas aeruginosa Gram Stain

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

13 thoughts on “Biochemical Test of Pseudomonas aeruginosa”

  1. lam retired professor as well Ag.Scientist of Plant Pathology promoting organic farming resources in integration with bacteria ,fungi and nematodes.

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  2. I did biochemical tests on several bacterial isolates and had different results. but after identifying the type of bacteria, the bacteria is Pseudomonas aeruginosa. Why do these bacteria have different biochemical tests even though they are one type of bacteria?

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    • Ya, there are some sources that say that they are encapsulated. So, we can say they are both capsulated and non-capsulated. They secrete a capsule-like polysaccharide called alginate according to Sumita Jain and Dennis E. Ohman 2005)

      Reply

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