Biochemical Test of Pseudomonas aeruginosa

by

Biochemical Test of Pseudomonas aeruginosa

Basic Characteristics Properties (Pseudomonas aeruginosa)
Capsule Some Non-Capsulated and Some are encapsulated (Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate- Sumita Jain and Dennis E. Ohman 2005)
Catalase Positive (+ve)
Cetrimide Test Positive (+ve)
Citrate Positive (+ve)
Coagulase Negative (-ve)
Flagella Flagellated (Single)
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Negative (-ve)
H2S Negative (-ve)
Hemolysis Beta Hemolytic
Indole Negative (-ve)
Motility Motile (Unipolar)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Oxidative
Oxidase Positive (+ve)
Pigment Positive (+ve) (Blue/Green)
Shape Rod
Spore Non-Sporing
TSIA (Triple Sugar Iron Agar) Alkali/Alkali (Red/Red)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of
Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
DNase Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Negative (-ve)
Hippurate Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Malonate Positive (+ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Negative (-ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions
Acetate Utilization Positive (+ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Positive (+ve)
Lysine Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

Pseudomonas aeruginosa Gram Stain

About Author

Photo of author

Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

12 thoughts on “Biochemical Test of Pseudomonas aeruginosa”

  3. I did biochemical tests on several bacterial isolates and had different results. but after identifying the type of bacteria, the bacteria is Pseudomonas aeruginosa. Why do these bacteria have different biochemical tests even though they are one type of bacteria?

    Reply

    • Ya, there are some sources that say that they are encapsulated. So, we can say they are both capsulated and non-capsulated. They secrete a capsule-like polysaccharide called alginate according to Sumita Jain and Dennis E. Ohman 2005)

      Reply

Leave a Comment