Biochemical Test of Kingella kingae

Biochemical Test of Kingella kingae

Some of the characteristics are as follows:

Basic Characteristics Properties (Kingella kingae)
10% Bile Negative (-ve)
Capsule Positive (+ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Coagulase Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining  Gram-negative
H2S Variable
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
6% NaCl Negative (-ve)
Nitrate Reduction Negative (-ve)
Nitrite Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Positive (+ve)
Shape Coccobacilli (0.6 to 1 μm by 1 to 3 μm)
Spore Negative (-ve)
Urease Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Arginine Dihydrolase Negative (-ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Lysine decarboxylase Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

References

  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-,
  2. and Epsilonproteobacteria
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284298/
  4. https://www.sciencedirect.com/topics/medicine-and-dentistry/kingella-kingae
  5. https://jcm.asm.org/content/51/5/1627.full.pdf
  6. https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/65202
  7. https://onlinelibrary.wiley.com/doi/full/10.1111/j.1469-0691.2012.03799.x

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

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