Biochemical Test of Kingella kingae

Biochemical Test of Kingella kingae

Some of the characteristics are as follows:

Basic Characteristics Properties (Kingella kingae)
10% Bile Negative (-ve)
Capsule Positive (+ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Coagulase Negative (-ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining  Gram-negative
H2S Variable
Hemolysis Positive (+ve)
Indole Negative (-ve)
Motility Negative (-ve)
6% NaCl Negative (-ve)
Nitrate Reduction Negative (-ve)
Nitrite Reduction Negative (-ve)
OF (Oxidative-Fermentative) Fermentative
Oxidase Positive (+ve)
Shape Coccobacilli (0.6 to 1 μm by 1 to 3 μm)
Spore Negative (-ve)
Urease Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Fructose Negative (-ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acid Phosphatase Positive (+ve)
Alkaline Phosphatase Positive (+ve)
Arginine Dihydrolase Negative (-ve)
Casein Hydrolysis Positive (+ve)
Esculin Hydrolysis Negative (-ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Lysine decarboxylase Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)


  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-,
  2. and Epsilonproteobacteria

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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