Biochemical Test of Haemophilus parainfluenzae

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Biochemical Test of Haemophilus parainfluenzae

Some of the characteristics are as follows:

Basic Characteristics

Properties (Haemophilus parainfluenzae)

Capsule Variable
Catalase Variable
Gas Variable
Gram Staining Gram-negative (-ve)
H2S Positive (+ve)
Hemolysis Negative (-ve)
Indole Variable
Motility Negative (-ve)
Nitrate Reduction Positive (+ve)
Nitrite Reduction Positive (+ve)
Oxidase Positive (+ve)
Shape Small, pleomorphic rods, usually with long, filamentous forms.
Urease Variable
V-factor requirement Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Glycerol Variable
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Raffinose Variable
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Sorbose Negative (-ve)
Sucrose Positive (+ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Alkaline Phosphatase Positive (+ve)
Arginine Dihydrolase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Fucosidase Negative (-ve)
IgA1 protease Negative (-ve)
Lysine decarboxylase Variable
ONPG (β-galactosidase) Variable
Ornithine Decarboxylase Variable

References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria.
  2. https://www.microbiologyresearch.org/docserver/fulltext/ijsem/28/1/ijs-28-1-20.pdf?expires=1555943745&id=id&accname=guest&checksum=62D277D20357271EE2C65A513EF17921
  3. https://www.sciencedirect.com/topics/medicine-and-dentistry/haemophilus-parainfluenzae
  4. https://jcm.asm.org/content/jcm/11/1/22.full.pdf
  5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275054/
  6. https://www.ncbi.nlm.nih.gov/pubmed/6979159
  7. https://www.cdc.gov/hi-disease/about/diagnosis-treatment.html

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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