Biochemical Test of Bordetella pertussis

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Biochemical Test of Bordetella pertussis

Some of the characteristics are as follows:

Basic Characteristics Properties (Bordetella pertussis)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in nutrient broth Negative (-ve)
Hemolysis Positive (+ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive(+ve)
Pigment Negative (-ve)
Shape Minute coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)

Fermentation of

Adipate Negative (-ve)
Caprate Negative (-ve)
Chymotrypsin activity Positive (+ve)
Gluconate Negative (-ve)
Glucose Negative (-ve)
Lactose Negative (-ve)
Malate Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Phenylacetate Negative (-ve)
Sucrose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Alkaline Phosphatase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Ester C8 lipase activity Positive (+ve)
Lipase C14 activity Negative (-ve)
Lysine decarboxylase Negative (-ve)
Naphthol-AS-B1 phosphohydrolase activity Positive (+ve)
Trypsin Negative (-ve)


  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. Part C. The Proteobacteria. New York: Springer.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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