Biochemical Test of Bordetella pertussis

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Bordetella pertussis

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Bordetella pertussis)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitrateNegative (-ve)
FlagellaNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-negative (-ve)
Growth in nutrient brothNegative (-ve)
HemolysisPositive (+ve)
MotilityNegative (-ve)
Nitrate ReductionNegative (-ve)
OxidasePositive(+ve)
PigmentNegative (-ve)
ShapeMinute coccobacilli
SporeNegative (-ve)
UreaseNegative (-ve)

Fermentation of

AdipateNegative (-ve)
CaprateNegative (-ve)
Chymotrypsin activityPositive (+ve)
GluconateNegative (-ve)
GlucoseNegative (-ve)
LactoseNegative (-ve)
MalateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
PhenylacetateNegative (-ve)
SucroseNegative (-ve)
XyloseNegative (-ve)

Enzymatic Reactions

Alkaline PhosphataseNegative (-ve)
Esculin HydrolysisNegative (-ve)
Ester C8 lipase activityPositive (+ve)
Lipase C14 activityNegative (-ve)
Lysine decarboxylaseNegative (-ve)
Naphthol-AS-B1 phosphohydrolase activityPositive (+ve)
TrypsinNegative (-ve)

References

  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. Part C. The Proteobacteria. New York: Springer.
  2. https://www.vetbact.org/?artid=55
  3. https://www.who.int/immunization/sage/meetings/2014/april/2_Laboratory_manual_WHO_2013_Update.pdf
  4. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Bordetella.htm
  5. https://cmr.asm.org/content/28/4/1005
  6. https://www.ncbi.nlm.nih.gov/books/NBK7813/
  7. https://nios.ac.in/media/documents/dmlt/Microbiology/Lesson-30.pdf

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