Biochemical Test of Bordetella pertussis

Biochemical Test of Bordetella pertussis

Some of the characteristics are as follows:

Basic Characteristics Properties (Bordetella pertussis)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in nutrient broth Negative (-ve)
Hemolysis Positive (+ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive(+ve)
Pigment Negative (-ve)
Shape Minute coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)

Fermentation of

Adipate Negative (-ve)
Caprate Negative (-ve)
Chymotrypsin activity Positive (+ve)
Gluconate Negative (-ve)
Glucose Negative (-ve)
Lactose Negative (-ve)
Malate Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Phenylacetate Negative (-ve)
Sucrose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Alkaline Phosphatase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Ester C8 lipase activity Positive (+ve)
Lipase C14 activity Negative (-ve)
Lysine decarboxylase Negative (-ve)
Naphthol-AS-B1 phosphohydrolase activity Positive (+ve)
Trypsin Negative (-ve)


  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. Part C. The Proteobacteria. New York: Springer.

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is currently doing his Ph.D. from the Central Department of Microbiology, Tribhuvan University in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He did his M.Sc. in Microbiology and B.Sc. in Microbiology from St. Xavier’s College, Kathmandu, Nepal. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from March 2017 to June 2019. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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