Biochemical Test of Bordetella pertussis

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Biochemical Test of Bordetella pertussis

Some of the characteristics are as follows:

Basic Characteristics Properties (Bordetella pertussis)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram-negative (-ve)
Growth in nutrient broth Negative (-ve)
Hemolysis Positive (+ve)
Motility Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Positive(+ve)
Pigment Negative (-ve)
Shape Minute coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)

Fermentation of
Adipate Negative (-ve)
Caprate Negative (-ve)
Chymotrypsin activity Positive (+ve)
Gluconate Negative (-ve)
Glucose Negative (-ve)
Lactose Negative (-ve)
Malate Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Phenylacetate Negative (-ve)
Sucrose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions
Alkaline Phosphatase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Ester C8 lipase activity Positive (+ve)
Lipase C14 activity Negative (-ve)
Lysine decarboxylase Negative (-ve)
Naphthol-AS-B1 phosphohydrolase activity Positive (+ve)
Trypsin Negative (-ve)

References

  1. Brenner, D. J., Krieg, N. R., Garrity, G. M., & Staley, J. T. (2005). Bergey’s manual of systematic bacteriology: Volume two. Part C. The Proteobacteria. New York: Springer.
  2. https://www.vetbact.org/?artid=55
  3. https://www.who.int/immunization/sage/meetings/2014/april/2_Laboratory_manual_WHO_2013_Update.pdf
  4. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Bordetella.htm
  5. https://cmr.asm.org/content/28/4/1005
  6. https://www.ncbi.nlm.nih.gov/books/NBK7813/
  7. https://nios.ac.in/media/documents/dmlt/Microbiology/Lesson-30.pdf

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).