Biochemical Test of Lactobacillus spp.

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Last Updated on January 1, 2020 by Sagar Aryal

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

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Some of the characteristics are as follows:

Basic CharacteristicsProperties (Lactobacillus spp.)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
FlagellaIf present, peritrichous
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram positive
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityMostly Negative (-ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
PigmentRare; if present, yellow or orange to rust or brick red.
Shapelong and slender, sometimes bent rods to short, often coryneform coccobacilli
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)

Fermentation of (For L. acidophilus)

AmygdalinPositive (+ve)
ArabinosePositive (+ve)
CellobiosePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
MelibioseVariable
RaffinoseVariable
RhamnosePositive (+ve)
RiboseNegative (-ve)
SalicinPositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehaloseVariable
XyloseVariable

Enzymatic Reactions

Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)

References

  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  2. https://scialert.net/fulltextmobile/?doi=ajbs.2016.1.9
  3. https://www.ijcmas.com/5-4-2016/P.%20Kavitha,%20et%20al.pdf
  4. http://www.bioline.org.br/request?cc05006
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.119
  6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/
  7. https://www.sciencedirect.com/science/article/pii/0740002085900206
  8. https://www.jstage.jst.go.jp/article/fstr/11/3/11_3_328/_pdf

Biochemical Test of Lactobacillus spp.

1 thought on “Biochemical Test of Lactobacillus spp.”

  1. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques

    Reply

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