Biochemical Test of Lactobacillus spp

Biochemical Test of Lactobacillus spp.

Last Updated on January 1, 2020 by Sagar Aryal

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

Image Source: Creative Market and India Mart.

Some of the characteristics are as follows:

Basic Characteristics Properties (Lactobacillus spp.)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella If present, peritrichous
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram positive
H2S Negative (-ve)
Indole Negative (-ve)
Motility Mostly Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Pigment Rare; if present, yellow or orange to rust or brick red.
Shape long and slender, sometimes bent rods to short, often coryneform coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of (For L. acidophilus)

Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Positive (+ve)
Ribose Negative (-ve)
Salicin Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Variable
Xylose Variable

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)

References

  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  2. https://scialert.net/fulltextmobile/?doi=ajbs.2016.1.9
  3. https://www.ijcmas.com/5-4-2016/P.%20Kavitha,%20et%20al.pdf
  4. http://www.bioline.org.br/request?cc05006
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.119
  6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/
  7. https://www.sciencedirect.com/science/article/pii/0740002085900206
  8. https://www.jstage.jst.go.jp/article/fstr/11/3/11_3_328/_pdf

Biochemical Test of Lactobacillus spp.

1 thought on “Biochemical Test of Lactobacillus spp.”

  1. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques

    Reply

Leave a Comment