Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp.
Biochemical Test of Lactobacillus spp.

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Some of the characteristics are as follows:

Basic CharacteristicsProperties (Lactobacillus spp.)
CapsuleNegative (-ve)
CatalaseNegative (-ve)
CitrateNegative (-ve)
FlagellaIf present, peritrichous
GasNegative (-ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingGram-positive (+ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityMostly Negative (-ve)
MR (Methyl Red)Mostly Negative (-ve), Some spp. Positive (+ve)
Nitrate ReductionNegative (-ve)
OxidaseNegative (-ve)
PigmentRare; if present, yellow or orange to rust or brick red.
Shapelong and slender, sometimes bent rods to short, often coryneform coccobacilli
SporeNegative (-ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Negative (-ve)
Fermentation of (For L. acidophilus)
AmygdalinPositive (+ve)
ArabinosePositive (+ve)
CellobiosePositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
LactosePositive (+ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannosePositive (+ve)
RhamnosePositive (+ve)
RiboseNegative (-ve)
SalicinPositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
Enzymatic Reactions
Casein HydrolysisNegative (-ve)
Esculin HydrolysisPositive (+ve)


  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi:

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

4 thoughts on “Biochemical Test of Lactobacillus spp.”

  1. methyl red test for lactobacillus spp. is positive,because it produce lactic acid after incubation . you’re wrong. please crt it.

  2. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques


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