Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

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Some of the characteristics are as follows:

Basic Characteristics Properties (Lactobacillus spp.)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella If present, peritrichous
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram positive
H2S Negative (-ve)
Indole Negative (-ve)
Motility Mostly Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Pigment Rare; if present, yellow or orange to rust or brick red.
Shape long and slender, sometimes bent rods to short, often coryneform coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)
Fermentation of (For L. acidophilus)
Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Positive (+ve)
Ribose Negative (-ve)
Salicin Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Variable
Xylose Variable
Enzymatic Reactions
Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)


  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi:

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

2 thoughts on “Biochemical Test of Lactobacillus spp.”

  1. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques


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