Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

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Some of the characteristics are as follows:

Basic Characteristics Properties (Lactobacillus spp.)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella If present, peritrichous
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram positive
H2S Negative (-ve)
Indole Negative (-ve)
Motility Mostly Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Pigment Rare; if present, yellow or orange to rust or brick red.
Shape long and slender, sometimes bent rods to short, often coryneform coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of (For L. acidophilus)

Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Positive (+ve)
Ribose Negative (-ve)
Salicin Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Variable
Xylose Variable

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)


  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009).¬†Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi:

Biochemical Test of Lactobacillus spp.

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