Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

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Some of the characteristics are as follows:

Basic Characteristics Properties (Lactobacillus spp.)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella If present, peritrichous
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram positive
H2S Negative (-ve)
Indole Negative (-ve)
Motility Mostly Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Pigment Rare; if present, yellow or orange to rust or brick red.
Shape long and slender, sometimes bent rods to short, often coryneform coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of (For L. acidophilus)

Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Positive (+ve)
Ribose Negative (-ve)
Salicin Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Variable
Xylose Variable

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)

References

  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  2. https://scialert.net/fulltextmobile/?doi=ajbs.2016.1.9
  3. https://www.ijcmas.com/5-4-2016/P.%20Kavitha,%20et%20al.pdf
  4. http://www.bioline.org.br/request?cc05006
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.119
  6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/
  7. https://www.sciencedirect.com/science/article/pii/0740002085900206
  8. https://www.jstage.jst.go.jp/article/fstr/11/3/11_3_328/_pdf

Biochemical Test of Lactobacillus spp.

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1 thought on “Biochemical Test of Lactobacillus spp.”

  1. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques

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