Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

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Some of the characteristics are as follows:

Basic Characteristics Properties (Lactobacillus spp.)
Capsule Negative (-ve)
Catalase Negative (-ve)
Citrate Negative (-ve)
Flagella If present, peritrichous
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Gram positive
H2S Negative (-ve)
Indole Negative (-ve)
Motility Mostly Negative (-ve)
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Negative (-ve)
Oxidase Negative (-ve)
Pigment Rare; if present, yellow or orange to rust or brick red.
Shape long and slender, sometimes bent rods to short, often coryneform coccobacilli
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)
Fermentation of (For L. acidophilus)
Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Positive (+ve)
Melibiose Variable
Raffinose Variable
Rhamnose Positive (+ve)
Ribose Negative (-ve)
Salicin Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Trehalose Variable
Xylose Variable
Enzymatic Reactions
Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)


  1. Bergey, D. H., Whitman, W. B., De, V. P., Garrity, G. M., & Jones, D. (2009). Bergey’s manual of systematic bacteriology: Vol. 3. New York: Springer.
  5. Kavitha, P., D. Sindhuja and Banumathi, M. 2016. Isolation and Biochemical Characterization of Lactobacillus species Isolated from Dahi. Int.J.Curr.Microbiol.App.Sci. 5(4): 1042-1049. doi:

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK).

2 thoughts on “Biochemical Test of Lactobacillus spp.”

  1. j’ai trés besoin de tableau de classification des bacteries lactiques
    veuillez m’envoyer si c possible la classification de toutes les bactéries lactiques


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