Lipid Hydrolysis Test- Objectives, Principle, Procedure and Results

Result Interpretation of Lipid Hydrolysis Test

Objectives of Lipid Hydrolysis Test To determine the ability of the organism to hydrolyse lipid. To identify bacteria capable of producing the exoenzyme lipase. Principle of Lipid Hydrolysis Test Lipids generally are nonpolar molecules that do not dissolve well in water.  Fats are one type of lipids that are large polymers of fatty acids and glycerol that are too large to enter the …

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Lecithinase Test- Objectives, Principle, Procedure and Results

Result Interpretation of Lecithinase Test

Objective of Lecithinase Test The objective is to determine the ability of microorganisms to produce the enzyme lecithinase and to identify the bacteria which are capable of producing lecithinase enzyme. Principle of Lecithinase Test Lecithinases or phospholipases are enzymes released by bacteria that have the ability to destroy animal tissues and play a role in pathogenecity. Lecithinase, which is also called phospholipase C, …

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Lysine Iron Agar Test

Result Interpretation of Lysine Iron Agar Test

Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar (LIA) contains lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate. The medium has an aerobic slant and an anaerobic butt. Lysine deamination is an aerobic process which occurs …

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Litmus Milk Medium Test

Result Interpretation of Litmus Milk Medium Test

Objective of Litmus Milk Medium Test To differentiate microorganisms based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas.  Litmus Milk is used primarily to differentiate members within the genus Clostridium. It differentiates Enterobacteriaceae from other Gram-negative bacilli based on the ability of enterics to reduce litmus. Litmus Milk also is used …

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Hydrogen Sulfide (H2S) Production Test

Result Interpretation of Hydrogen Sulfide (H2S) Production Test

Objective of Hydrogen Sulfide (H2S) Production Test To determine the ability of the organism to produce hydrogen sulphide. Principle of Hydrogen Sulfide (H2S) Production Test Hydrogen sulphide (H2S) production test is used for the detection of hydrogen sulphide (H2S) gas produced by an organism. It is used mainly to assist in the identification of members of family Enterobacteriaceae. H2S is produced by certain …

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Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are inhibited by the bile salts …

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