Hippurate Hydrolysis Test- Principle, Procedure and Result Interpretation

Result Interpretation of Hippurate Hydrolysis Test

The objective of Hippurate Hydrolysis Test Production of the enzyme hippuricase is used for the presumptive identification of a variety of microorganisms. The hippurate test is used in the identification of Campylobacter jejuni, Listeria monocytogenes, Gardnerella vaginalis and Streptococcus agalactiae by detecting the ability of the organism to hydrolyze sodium hippurate to benzoic acid and glycine by the action of the enzyme …

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Growth at 42°C Test- Principle, Procedure and Result Interpretation

Growth at 42°C Test- Principle, Procedure and Result Interpretation

Growth at 42°C Test- Principle, Procedure and Result Interpretation Purpose of Growth at 42°C Test This test is used to differentiate a pyocyanogenic pseudomonads from other Pseudomonas sp. Principle of Growth at 42°C Test The test is used to determine the ability of an organism to grow at 42°C. Several Pseudomonas species have been isolated in the clinical laboratory that are capable …

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Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation

Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation

Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation Objective of Gelatin Hydrolysis Test The production of gelatinases capable of hydrolyzing gelatin is used as a presumptive test for the identification of various organisms, including Staphylococcus sp., Enterobacteriaceae, and some gram-positive bacilli. Principle of Gelatin Hydrolysis Test This test is used to determine the ability of an organism to produce extracellular proteolytic enzymes …

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Esculin Hydrolysis Test- Principle, Procedure, Results

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Objective of Esculin Hydrolysis Test This test is used for the presumptive identification and differentiation of Enterobacteriaceae. Principle of Esculin Hydrolysis Test This test is used to determine whether an organism is able to hydrolyze the glycoside esculin. Esculin is hydrolyzed to esculetin, which reacts with Fe3+ and forms a dark brown to black precipitate. Media: NaCl (8 g), K2HPO4 (0.4 …

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DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation Objectives of DNase Test Agar (DNA Hydrolysis) This test is used to differentiate organisms based on the production of deoxyribonuclease. It is used to distinguish Serratia spp (positive) from Enterobacter spp., Staphylococcus aureus (positive) from other species, and Moraxella catarrhalis (positive) from Neisseria sp. Principle of DNase Test Agar (DNA Hydrolysis) The test is used …

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Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are inhibited by the bile salts …

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