Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

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Some of the characteristics are as follows:

Basic Characteristics Properties (Haemophilus influenzae)
Capsule Strain-dependent
Catalase Positive (+ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gram Staining Gram-negative
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Variable
Motility Negative (-ve)
Nitrate Reduction Positive (+ve)
Nitrite Reduction Negative (-ve)
Oxidase Positive (+ve)
Pigment Absent or yellowish
Shape Coccobacilli or small regular rods
Urease Variable
V-factor requirement Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Negative (-ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Sorbose Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Positive (+ve)

Enzymatic Reactions

Alkaline Phosphatase Positive (+ve)
Arginine Dihydrolase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Fucosidase Negative (-ve)
IgA1 protease Positive (+ve)
Lysine decarboxylase Variable
Neuraminidase Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Variable

 References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria.
  2. https://microbiologyinfo.com/haemophilus-influenzae/
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275054/
  4. https://www.cdc.gov/meningitis/lab-manual/chpt09-id-characterization-hi.pdf
  5. https://jcm.asm.org/content/jcm/7/6/519.full.pdf
  6. https://jcm.asm.org/content/jcm/11/1/22.full.pdf

Biochemical Test of Haemophilus influenzae

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