Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

Last Updated on January 7, 2020 by Sagar Aryal

Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

Image Source: Manitoba Health and Fine Art America.

Some of the characteristics are as follows:

Basic Characteristics Properties (Haemophilus influenzae)
Capsule Strain-dependent
Catalase Positive (+ve)
Flagella Negative (-ve)
Gas Negative (-ve)
Gram Staining Gram-negative
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Variable
Motility Negative (-ve)
Nitrate Reduction Positive (+ve)
Nitrite Reduction Negative (-ve)
Oxidase Positive (+ve)
Pigment Absent or yellowish
Shape Coccobacilli or small regular rods
Urease Variable
V-factor requirement Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Cellobiose Negative (-ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Fructose Negative (-ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Positive (+ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Sorbose Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Positive (+ve)

Enzymatic Reactions

Alkaline Phosphatase Positive (+ve)
Arginine Dihydrolase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Fucosidase Negative (-ve)
IgA1 protease Positive (+ve)
Lysine decarboxylase Variable
Neuraminidase Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Variable

 References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria.
  2. https://microbiologyinfo.com/haemophilus-influenzae/
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275054/
  4. https://www.cdc.gov/meningitis/lab-manual/chpt09-id-characterization-hi.pdf
  5. https://jcm.asm.org/content/jcm/7/6/519.full.pdf
  6. https://jcm.asm.org/content/jcm/11/1/22.full.pdf

Biochemical Test of Haemophilus influenzae

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