Biochemical Test of Haemophilus influenzae

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Last Updated on January 7, 2020 by Sagar Aryal

Biochemical Test of Haemophilus influenzae

Biochemical Test of Haemophilus influenzae

Image Source: Manitoba Health and Fine Art America.

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Haemophilus influenzae)
CapsuleStrain-dependent
CatalasePositive (+ve)
FlagellaNegative (-ve)
GasNegative (-ve)
Gram StainingGram-negative
H2SNegative (-ve)
HemolysisNegative (-ve)
IndoleVariable
MotilityNegative (-ve)
Nitrate ReductionPositive (+ve)
Nitrite ReductionNegative (-ve)
OxidasePositive (+ve)
PigmentAbsent or yellowish
ShapeCoccobacilli or small regular rods
UreaseVariable
V-factor requirementPositive (+ve)

Fermentation of

AdonitolNegative (-ve)
ArabinoseNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructoseNegative (-ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolNegative (-ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
SorboseNegative (-ve)
SucroseNegative (-ve)
TrehaloseNegative (-ve)
XylosePositive (+ve)

Enzymatic Reactions

Alkaline PhosphatasePositive (+ve)
Arginine DihydrolaseNegative (-ve)
Esculin HydrolysisNegative (-ve)
FucosidaseNegative (-ve)
IgA1 proteasePositive (+ve)
Lysine decarboxylaseVariable
NeuraminidasePositive (+ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylaseVariable

 References

  1. Bergey’s Manual of Systematic Bacteriology Volume 2: The Proteobacteria, Part B:The Gammaproteobacteria.
  2. https://microbiologyinfo.com/haemophilus-influenzae/
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275054/
  4. https://www.cdc.gov/meningitis/lab-manual/chpt09-id-characterization-hi.pdf
  5. https://jcm.asm.org/content/jcm/7/6/519.full.pdf
  6. https://jcm.asm.org/content/jcm/11/1/22.full.pdf

Biochemical Test of Haemophilus influenzae

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