Nitrate Reduction Test- Objectives, Principle, Procedure and Results

Result Interpretation of Nitrate Reduction Test

Objectives of Nitrate Reduction Test To determine the ability organism to produce an enzyme called nitrate reductase, resulting in the reduction of nitrate (NO3). It also tests the ability of organisms to perform nitrification on nitrate and nitrite to produce molecular nitrogen. Principle of Nitrate Reduction Test All members of the Enterobacteriaceae family reduce nitrate to nitrite, but … Read moreNitrate Reduction Test- Objectives, Principle, Procedure and Results

Microdase (Modified Oxidase) Test- Principle, Procedure and Results

Result Interpretation of Microdase (Modified Oxidase) Test

Objective of Microdase (Modified Oxidase) Test This test is used to differentiate gram-positive, catalase-positive cocci (micrococci from staphylococci). Principle of Microdase (Modified Oxidase) Test The microdase test, also known as modified oxidase test is a rapid test to differentiate Staphylococcus from Micrococcus which are Gram positive cocci possessing catalase enzyme. The differentiation is based on the detection of oxidase … Read moreMicrodase (Modified Oxidase) Test- Principle, Procedure and Results

Methyl Red (MR) Test- Objectives, Principle, Media Used, Procedure, Result Interpretation, Limitations and Examples

Result Interpretation of Methyl Red (MR) Test

Objectives of Methyl Red (MR) Test To differentiate two major types of facultative anaerobic enteric bacteria based on production of acid. It identifies bacterial ability to produce stable acid end products by means of a mixed-acid fermentation of glucose. Principle of Methyl Red (MR) Test Methyl red test, commonly known as MR test is used to determine the … Read moreMethyl Red (MR) Test- Objectives, Principle, Media Used, Procedure, Result Interpretation, Limitations and Examples

Malonate Test- Objectives, Principle, Procedure and Results

Result Interpretation of Malonate Test

Objectives of Malonate Test To test the ability of the organism to utilize malonate as sole source of carbon. The Malonate Test was originally designed to differentiate between Escherichia, which will not grow in the medium, and Enterobacter. Its use as a differential medium has now broadened to include other members of Enterobacteriaceae. Principle of Malonate Test An … Read moreMalonate Test- Objectives, Principle, Procedure and Results

Lipid Hydrolysis Test- Objectives, Principle, Procedure and Results

Result Interpretation of Lipid Hydrolysis Test

Objectives of Lipid Hydrolysis Test To determine the ability of the organism to hydrolyse lipid. To identify bacteria capable of producing the exoenzyme lipase. Principle of Lipid Hydrolysis Test Lipids generally are nonpolar molecules that do not dissolve well in water.  Fats are one type of lipids that are large polymers of fatty acids and glycerol that are … Read moreLipid Hydrolysis Test- Objectives, Principle, Procedure and Results

Lecithinase Test- Objectives, Principle, Procedure and Results

Result Interpretation of Lecithinase Test

Objective of Lecithinase Test The objective is to determine the ability of microorganisms to produce the enzyme lecithinase and to identify the bacteria which are capable of producing lecithinase enzyme. Principle of Lecithinase Test Lecithinases or phospholipases are enzymes released by bacteria that have the ability to destroy animal tissues and play a role in pathogenecity. Lecithinase, which … Read moreLecithinase Test- Objectives, Principle, Procedure and Results

Lipase Test- Objectives, Principle, Procedures and Results

Result Interpretation of Lipase Test

Objectives of Lipase Test The objective is to identify bacteria capable of producing the exoenzyme lipase. The Lipase Test is used to detect and enumerate lipolytic bacteria, especially in high-fat dairy products. A variety of other lipid substrates, including corn oil, olive oil, and soybean oil, are used to detect differential characteristics among members of Enterobacteriaceae, … Read moreLipase Test- Objectives, Principle, Procedures and Results

Lysine Iron Agar Test

Result Interpretation of Lysine Iron Agar Test

Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar (LIA) contains lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate. The medium has an aerobic slant and an anaerobic butt. … Read moreLysine Iron Agar Test

Litmus Milk Medium Test

Result Interpretation of Litmus Milk Medium Test

Objective of Litmus Milk Medium Test To differentiate microorganisms based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas.  Litmus Milk is used primarily to differentiate members within the genus Clostridium. It differentiates Enterobacteriaceae from other Gram-negative bacilli based on the ability of enterics to reduce litmus. … Read moreLitmus Milk Medium Test

Hydrogen Sulfide (H2S) Production Test

Result Interpretation of Hydrogen Sulfide (H2S) Production Test

Objective of Hydrogen Sulfide (H2S) Production Test To determine the ability of the organism to produce hydrogen sulphide. Principle of Hydrogen Sulfide (H2S) Production Test Hydrogen sulphide (H2S) production test is used for the detection of hydrogen sulphide (H2S) gas produced by an organism. It is used mainly to assist in the identification of members of family Enterobacteriaceae. … Read moreHydrogen Sulfide (H2S) Production Test