Novobiocin Susceptibility Test- Principle, Procedure, Results, Uses

Result Interpretation of Novobiocin Susceptibility Test

Objectives of Novobiocin Susceptibility Test To determine the susceptibility pattern of a bacterium to novobiocin. To distinguish or differentiate Staphylococcus saprophyticus from other coagulase-negative staphylococci. Principle of Novobiocin Susceptibility Test Novobiocin is an antibiotic interfering with the unpackaging and repackaging of DNA during DNA replication and the bacterial cell cycle. Novobiocin binds to DNA gyrase and blocks adenosine triphosphatase (ATPase) activity.  Laboratory identification of S. …

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Methyl Red (MR) Test- Principle, Procedure, Results, Uses

Result Interpretation of Methyl Red (MR) Test

Objectives of Methyl Red (MR) Test To differentiate two major types of facultative anaerobic enteric bacteria based on the production of acid. It identifies bacterial ability to produce stable acid end products by means of a mixed-acid fermentation of glucose. Principle of Methyl Red (MR) Test Methyl red test, commonly known as MR test is used to determine the ability of an organism …

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Decarboxylase Test- Principle, Procedure, Results, Uses

Decarboxylase Test

Decarboxylase Test Definition Decarboxylase test is a biochemical test performed to differentiate members of Enterobacteriaceae on the basis of their ability to produce the enzyme decarboxylase. The metabolism of amino acids might differ in aerobic and facultatively anaerobic bacteria as well as in gram-negative organisms. Metabolism of amino acid might occur either by decarboxylation, hydrolysis, or deamination, depending on the …

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Coagulase Test- Principle, Procedure, Types, Result, Uses

Coagulase Test

Coagulase Test Definition A coagulase test is a biochemical test that is used to differentiate Staphylococcus aureus from other Staphylococci species like S. epidermidis and S. saprophyticus on the basis of the ability to produce the coagulase enzyme. The coagulase test is an important test that differentiates the species of the genus Staphylococci into two groups; Coagulase positive Staphylococci and …

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Citrate Utilization Test- Principle, Procedure, Results, Uses

Citrate Utilization Test- Principle, Procedure and Result Interpretation

What is Citrate Utilization Test? The citrate utilization test is a part of the IMViC test (Indole, Methyl Red, Vogues-Proskauer, and Citrate Test) that differentiates organisms on the basis of their ability to use citrate as a sole source of energy. The citrate test is performed along with the other IMViC tests to differentiate Gram-negative bacilli of the Enterobacteriaceae family. …

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Cetrimide Agar Test- Principle, Procedures, Results, Uses

Cetrimide Agar Test- Principle, Procedure and Result Interpretation

What is Cetrimide Agar Test? Cetrimide agar test is a biochemical test performed to identify or differentiate Pseudomonas aeruginosa from other microorganisms. The test works on the principle of the ability of an organism to grow in the presence of cetrimide. Cetrimide is a toxic quaternary ammonium detergent that is toxic to most bacteria except for few organisms like P. …

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Catalase Test- Principle, Procedure, Types, Results, Uses

Catalase Test- Slide Method

What is Catalase Test? The catalase test is a biochemical test for aerobic organisms that detects the production of catalase enzyme in the organism. Catalase enzyme is a common enzyme that is found in all living beings that survive in oxygen and catalyzes the decomposition of hydrogen peroxide, releasing water and oxygen. Catalase is an essential enzyme in pathogenic organisms …

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CAMP Test- Principle, Procedure, Types, Results, Uses, Limitations

CAMP Test- Principle, Procedure and Result Interpretation

What is CAMP Test? CAMP (Christie–Atkins–Munch-Peterson) test is a biochemical test used to differentiate Streptococcus agalactiae from other beta-hemolytic Streptococci. S. agalactiae is the only member of the Group B Streptococci which is separated from other beta-hemolytic Streptococci by the demonstration of a large area of hemolysis when grown with Staphylococcus aureus. This test is used for the presumptive identification …

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Butyrate Disk Test- Principle, Procedure, Results, Uses, Limitations

Butyrate Disk Test- Principle, Procedure and Result Interpretation

What is Butyrate Disk Test? Butyrate disk test is a rapid identification test for the detection of Moraxella catarrhalis on the basis of their ability to utilize bromochloro-indolyl butyrate or 4-methylumbelliferyl butyrate. Moraxella (Branhamella) catarrhalis is now recognized as a major pathogen that causes otitis media, sinusitis, conjunctivitis, bronchitis, and pneumonia. In addition, a large number of M. catarrhalis strains …

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Bile Solubility Test- Principle, Procedure, Types, Results, Uses

Bile Solubility Test- Principle, Procedure and Result Interpretation

What is Bile Solubility Test? Bile solubility test is a biochemical test used for the differentiation and confirmation of Streptococcus pneumoniae from other alpha-hemolytic Streptococci. The bile solubility test has been used as an essential test for the differentiation of S. pneumoniae as it allows the distinction between the two species S. pneumoniae and Streptococcus pseudopneumoniae, which is a challenging task. The …

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