Proteins Archives | Microbe Notes

Solubility Tests of Proteins- Definition, Principle, Procedure, Result, Uses

November 26, 2020 by Anupama Sapkota

Solubility Tests of Proteins Definition Solubility tests are tests performed to determine the ability of compounds to dissolve in a solvent, which is usually a liquid. These tests are essential to determine the size and polarity of unknown compounds and the presence of acidic and basic functional groups. Solubility tests for proteins and amino acids are qualitative tests that determine …

Hopkin’s Cole Test- Definition, Principle, Procedure, Result, Uses

November 23, 2020 by Anupama Sapkota

Hopkin’s Cole Test Definition Hopkin’s Cole test is a specific test used for the detection of indole ring and thus, tryptophan in proteins. The test is also termed as ‘glyoxylic acid test’ as the reagent contains glyoxylic acid. The test was discovered by Frederick Gowland Hopkins and Sydney W. Cole in 1901 as a part of their work on the …

Heller’s Test- Definition, Principle, Procedure, Result, Uses

November 23, 2020 by Anupama Sapkota

Heller’s Test Definition Heller’s test is a biochemical test performed to detect proteins in a sample by the denaturation of those proteins by the addition of strong acids. Heller’s test usually uses concentrated nitric acid for the denaturation of proteins. The test is performed for clinical purposes to detect abnormal proteins in biological fluids, including urine. Heller’s test is a …

Heat coagulation test of proteins

November 23, 2020 by Anupama Sapkota

Heat coagulation test of proteins definition Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of proteins as a response to heat is a common phenomenon. The heat coagulation of proteins occurs in one of the two stages; denaturation and agglutination or the separation of the …

Esbach Test- Definition, Principle, Procedure, Result, Uses

November 23, 2020 by Anupama Sapkota

Esbach test definition Esbach test is one of the oldest biochemical tests used to detect urinary protein like albumin when the urine is combined with citric and picric acid. Esbach test, even though not discussed much through the history, is useful for both qualitative and quantitative determination of albumin in the urine sample. The test was discovered by Georges Hubert …

Biuret Test for Protein

May 5, 2020 by Sagar Aryal

What does Biuret test for? Proteins are the complex compound formed by thousands of amino acids. Amino acids are amphoteric electrolytes having carboxyl and amino groups that act like acid and base. It has one positive charge and one negative charge, hence these ions are electrically neutral and do not migrate in the electric field. The two amino acids are …

Membrane Proteins

February 4, 2021November 23, 2019 by Sagar Aryal

Biological membranes consist of a continuous double layer of lipid molecules in which membrane proteins are embedded. Although the lipid bilayer provides the basic structure of biological membranes, the membrane proteins perform most of the membrane’s specific tasks and therefore give each type of cell membrane its characteristic functional properties. The amounts and types of proteins in a membrane are …

Digestion and Absorption of Carbohydrates, Proteins and Fats

July 18, 2020May 25, 2019 by Sagar Aryal

Digestion and Absorption of Carbohydrates, Proteins, and Fats Carbohydrates, fats, and proteins are the major nutrients the body needs for growth, repair, movement, and maintaining tissue and organ function. These macromolecules are broken down and absorbed into the body at different rates and into specific forms as they travel through the organs in the digestive system. Digestion of carbohydrates Among …

Homology Modeling- Working, Steps, and Uses

February 4, 2021March 15, 2019 by Sagar Aryal

Homology modeling is also known as comparative modeling predicts protein structures based on sequence homology with known structures. It is based on the principle that “if two proteins share a high enough sequence similarity, they are likely to have very similar three-dimensional structures.” It hence relies on the identification of one or more known protein structures likely to resemble the …

Proteins- Properties, Structure, Classification and Functions

February 4, 2021August 6, 2018 by Sagar Aryal

Proteins are the most abundant biological macromolecules, occurring in all cells. It is also the most versatile organic molecule of the living systems and occur in great variety; thousands of different kinds, ranging in size from relatively small peptides to large polymers. Proteins are the polymers of amino acids covalently linked by the peptide bonds. The building blocks of proteins …