What does Biuret test for? Proteins are the complex compound formed by thousands of amino acids. Amino acids are amphoteric electrolytes having carboxyl and amino groups that act like acid and base. It has one positive charge and one negative … Read more
Proteins are the most abundant biological macromolecules, occurring in all cells. It is also the most versatile organic molecule of the living systems and occur in great variety; thousands of different kinds, ranging in size from relatively small peptides to … Read more
Biological membranes consist of a continuous double layer of lipid molecules in which membrane proteins are embedded. Although the lipid bilayer provides the basic structure of biological membranes, the membrane proteins perform most of the membrane’s specific tasks and therefore … Read more
Solubility Tests of Proteins Definition Solubility tests are tests performed to determine the ability of compounds to dissolve in a solvent, which is usually a liquid. These tests are essential to determine the size and polarity of unknown compounds and … Read more
Hopkin’s Cole Test Definition Hopkin’s Cole test is a specific test used for the detection of indole ring and thus, tryptophan in proteins. The test is also termed as ‘glyoxylic acid test’ as the reagent contains glyoxylic acid. The test … Read more
Heller’s Test Definition Heller’s test is a biochemical test performed to detect proteins in a sample by the denaturation of those proteins by the addition of strong acids. Heller’s test usually uses concentrated nitric acid for the denaturation of proteins. … Read more
Heat coagulation test of proteins definition Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of proteins as a response to heat is a common phenomenon. … Read more
Esbach test definition Esbach test is one of the oldest biochemical tests used to detect urinary protein like albumin when the urine is combined with citric and picric acid. Esbach test, even though not discussed much through the history, is … Read more
Digestion and Absorption of Carbohydrates, Proteins, and Fats Carbohydrates, fats, and proteins are the major nutrients the body needs for growth, repair, movement, and maintaining tissue and organ function. These macromolecules are broken down and absorbed into the body at … Read more
Homology modeling is also known as comparative modeling predicts protein structures based on sequence homology with known structures. It is based on the principle that “if two proteins share a high enough sequence similarity, they are likely to have very … Read more
Four levels of hierarchy in protein conformation can be described. A. PRIMARY STRUCTURE PRIMARY STRUCTURE refers to the order of the amino acids in the peptide chain. 1. The free α-amino group, written to the left, is called the amino-terminal … Read more