Calcofluor White Staining

Calcofluor White Staining

Calcofluor White Staining Calcofluor white is a chemifluorescent blue dye that is nonspecifically used to bind to the beta linked-polysaccharide polymers of amoebic cysts. It functions by being able to bind to 1-3 beta and 1-4 beta polysaccharides on chitin and cellulose that is present in cell walls on fungi, plants, and algae. Due to … Read more Calcofluor White Staining

Grocott-Gomori’s Methenamine Silver Staining

Grocott-Gomori's Methenamine Silver Staining

Grocott-Gomori’s Methenamine Silver (GMS) stain is a histological stain that is used majorly for the identification of carbohydrates in fungal microorganisms. This staining method was named after György Gömöri, a physician from Hungary, who developed the staining methodology. Its initial application to assess missing tissues and diseases in the liver and the rectum (Nadworny, Wang, … Read more Grocott-Gomori’s Methenamine Silver Staining

Yeast Artificial Chromosomes (YACs)

Yeast Artificial Chromosomes (YACs)

Yeast Artificial Chromosomes (YACs) Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast. It is a human-engineered DNA molecule used to clone DNA sequences in yeast cells. They are the products of a recombinant DNA cloning methodology to isolate and propagate very large segments of DNA in a yeast host. … Read more Yeast Artificial Chromosomes (YACs)

Classification of Fungi

Classification of Fungi

Classification of Fungi Fungi are eukaryotic microorganisms. They can occur as yeasts, molds, or as a combination of both forms. Some fungi are capable of causing superficial, cutaneous, subcutaneous, systemic or allergic diseases. Yeasts are microscopic fungi consisting of solitary cells that reproduce by budding. Molds, in contrast, occur in long filaments known as hyphae, … Read more Classification of Fungi

Differences between Yeasts and Molds (Yeasts vs Molds)

Differences between Yeasts and Molds

Differences between Yeasts and Molds Both yeast and moulds are eukaryotes – organisms with cell nuclei and membrane-bound organelles in the kingdom Fungi. The major differences between Yeasts and Molds are: S.N. Character Yeasts Molds 1.       Definition Yeast is a unicellular, budding fungus. Mold is a multicellular, threadlike fungus. 2.       Form Grow as … Read more Differences between Yeasts and Molds (Yeasts vs Molds)

Candida albicans

Habitat of Candida albicans Normal Habitat: mucosal membranes of human and other warm blooded animals. Also found in the gut, the vagina or also in the surface of the skin. Found in the digestive tract of birds also. Isolated from soil, animal, hospitals, in-animate objects and food. Worldwide distribution Morphology of Candida albicans Small, oval, … Read more Candida albicans

Characteristics of Fungi

Characteristics of Fungi

Characteristics of Fungi Fungi are eukaryotic organisms means they have true nucleus which are enclosed in membranes. They are non-vascular organisms. They do not have vascular system. Xylem and Phloem are absent. Fungi have cell walls (plants also have cell walls, but animals have no cell walls). There is no embryonic stage for fungi. They … Read more Characteristics of Fungi