Biochemical Test of Bacteria Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are … Read more Biochemical Test of Bacteria
Biochemical Test of Fusobacterium necrophorum Image Source: Microbe-Canvas Some of the characteristics are as follows: Basic Characteristics Properties (Fusobacterium necrophorum) Bile Growth Variable Capsule Negative (-ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Variable Gram Staining Gram-negative bacteria H2S Positive (+ve) Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed) Indole … Read more Biochemical Test of Fusobacterium necrophorum
Biochemical Test of Aeromonas hydrophila Image Source: Wikipedia Some of the characteristics are as follows: Basic Characteristics Properties (Aeromonas hydrophila) Capsule -ve Catalase +ve Citrate +ve Flagella +ve Gas +ve Gelatin Hydrolysis +ve Gram Staining Gram-negative H2S +ve Hemolysis +ve Indole +ve Maximum Growth Temperature 41°C Motility +ve Nitrate Reduction +ve OF (Oxidative-Fermentative) Fermentative Oxidase … Read more Biochemical Test of Aeromonas hydrophila
Biochemical Test of Haemophilus aegyptius Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus aegyptius) Capsule Negative (-ve) Catalase Positive (+ve) Gas Negative (-ve) Gram Staining .Gram-negative H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Negative (-ve) Oxidase Positive (+ve) Shape Long slender … Read more Biochemical Test of Haemophilus aegyptius
Biochemical Test of Burkholderia cepacia a: Burkholderia cepacia ATCC (Image Source: ATCC) b: Burkholderia cepacia (Image Source: Rapid Microbiology) c: Burkholderia cepacia (Image Source: OMICS International) d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific) e: Scanning Electron Micrograph of Burkholderia cepacia (Image Source: Wikipedia) f: Burkholderia cepacia … Read more Biochemical Test of Burkholderia cepacia
Biochemical Test of Klebsiella granulomatis Image: Granuloma inguinale (donovanosis) in a conventional Pap test. A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes. B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b). C: Macrophages show numerous safety pin-shaped structures … Read more Biochemical Test of Klebsiella granulomatis
Biochemical Test of Haemophilus parainfluenzae Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus parainfluenzae) Capsule Variable Catalase Variable Gas Variable Gram Staining Gram-negative (-ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Variable Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Positive (+ve) Oxidase Positive (+ve) Shape Small, pleomorphic rods, usually with … Read more Biochemical Test of Haemophilus parainfluenzae
Biochemical Test of Francisella tularensis subsp. tularensis Image Source: New York State Health Department Some of the characteristics are as follows: Basic Characteristics Properties (Francisella tularensis subsp. tularensis) Capsule Positive (+ve) Catalase Positive (+ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Faintly stained Gram-negative H2S (in cysteine-supplemented medium) Positive (+ve) … Read more Biochemical Test of Francisella tularensis subsp. tularensis
Biochemical Test of Kingella kingae Some of the characteristics are as follows: Basic Characteristics Properties (Kingella kingae) 10% Bile Negative (-ve) Capsule Positive (+ve) Catalase Negative (-ve) Citrate Negative (-ve) Coagulase Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative H2S Variable Hemolysis Positive (+ve) Indole Negative (-ve) Motility Negative (-ve) 6% … Read more Biochemical Test of Kingella kingae
Biochemical Test of Bordetella pertussis Some of the characteristics are as follows: Basic Characteristics Properties (Bordetella pertussis) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative (-ve) Growth in nutrient broth Negative (-ve) Hemolysis Positive (+ve) Motility Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Positive(+ve) … Read more Biochemical Test of Bordetella pertussis