Interesting Science Videos
Biochemical Test of Bacillus cereus
| Basic Characteristics | Properties (Bacillus cereus) |
| Catalase | Positive (+ve) |
| Citrate | Positive (+ve) |
| Gelatin Hydrolysis | Negative (-ve) |
| Gram Staining | Positive (+ve) |
| Growth in KCN | Positive (+ve) |
| Hemolysis | Positive (+ve) |
| Indole | Negative (-ve) |
| Motility | Positive (+ve) |
| MR (Methyl Red) | Negative (-ve) |
| Nitrate Reduction | Variable |
| Oxidase | Negative (-ve) |
| Pigment | Negative (-ve) |
| Shape | Rods |
| Spore | Positive (+ve) |
| VP (Voges Proskauer) | Positive (+ve) |
Fermentation of |
|
| Adonitol | Negative (-ve) |
| Arabinose | Negative (-ve) |
| Arabitol | Negative (-ve) |
| Cellobiose | Variable |
| Fructose | Positive (+ve) |
| Galactose | Negative (-ve) |
| Glucose | Positive (+ve) |
| Glycerol | Positive (+ve) |
| Glycogen | Positive (+ve) |
| Inositol | Negative (-ve) |
| Inulin | Negative (-ve) |
| Lactose | Negative (-ve) |
| Maltose | Positive (+ve) |
| Mannitol | Negative (-ve) |
| Mannose | Negative (-ve) |
| Melibiose | Negative (-ve) |
| Raffinose | Negative (-ve) |
| Rhamnose | Negative (-ve) |
| Ribose | Positive (+ve) |
| Salicin | Variable |
| Sorbitol | Negative (-ve) |
| Starch | Positive (+ve) |
| Sucrose | Variable |
| Trehalose | Positive (+ve) |
| Xylose | Negative (-ve) |
Enzymatic Reactions |
|
| Acetate Utilization | Variable |
| Arginine Dehydrolase | Variable |
| Casein Hydrolysis | Positive (+ve) |
| Esculin Hydrolysis | Positive (+ve) |
| Lecithinase | Positive (+ve) |
| Lysine | Negative (-ve) |
| Ornithine Decarboxylase | Negative (-ve) |
| Phenylalanine Deaminase | Negative (-ve) |
| Tyrosine Hydrolysis | Positive (+ve) |
Is it possible bacillus cereus can grow on mannitol salt agar?
I have isolated gram positive bacilli with short chains and showing yellow and light pink colinies on MSA.
Bacillus cereus can often tolerate and grow on mannitol salt agar (MSA), but it typically does not ferment mannitol, producing pink or red colonies rather than yellow. If you are seeing both yellow and pink colonies, you may have a mixed culture—one organism able to ferment mannitol (turning the medium yellow) and Bacillus species (likely producing pink colonies).
is bacillus.cereus hyporate positive ?
in my test it became negative!
Sorry, we did not got with the term “hyporate positive”. Can you please check once.