Biochemical Test of Bacillus cereus

Biochemical Test of Bacillus cereus
Biochemical Test of Bacillus cereus
­Basic CharacteristicsProperties (Bacillus cereus)
CatalasePositive (+ve)
CitratePositive (+ve)
Gelatin HydrolysisNegative (-ve)
Gram StainingPositive (+ve)
Growth in KCNPositive (+ve)
HemolysisPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionVariable
OxidaseNegative (-ve)
PigmentNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseVariable
VP (Voges Proskauer)Positive (+ve)
Fermentation of
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseVariable
FructosePositive (+ve)
GalactoseNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
InositolNegative (-ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltosePositive (+ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinVariable
SorbitolNegative (-ve)
StarchPositive (+ve)
SucroseVariable
TrehalosePositive (+ve)
XyloseNegative (-ve)
Enzymatic Reactions
Acetate UtilizationVariable
Arginine DehydrolaseVariable
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinasePositive (+ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tyrosine HydrolysisPositive (+ve)

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

8 thoughts on “Biochemical Test of Bacillus cereus”

  1. Is it possible bacillus cereus can grow on mannitol salt agar?
    I have isolated gram positive bacilli with short chains and showing yellow and light pink colinies on MSA.

    Reply
    • Bacillus cereus can often tolerate and grow on mannitol salt agar (MSA), but it typically does not ferment mannitol, producing pink or red colonies rather than yellow. If you are seeing both yellow and pink colonies, you may have a mixed culture—one organism able to ferment mannitol (turning the medium yellow) and Bacillus species (likely producing pink colonies).

      Reply

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