Biochemical Test of Enterobacter cloacae

Biochemical Test of Enterobacter cloacae

­Basic Characteristics Properties (Enterobacter cloacae)
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas from glucose Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Positive (+ve)
H2S Negative (-ve)
Indole Negative (-ve)
Motility Motile
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative Anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
D-Arabitol Variable
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Meso-Erythritol Negative (-ve)
Glucose Positive (+ve)
Glycerol Variable
Lactose Positive (+ve)
Maltose Positive (+ve)
D-Mannitol Positive (+ve)
D-Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Positive (+ve)
MyoInositol Negative (-ve)
Raffinose Positive (+ve)
Rhamnose Positive (+ve)
Salicin Positive (+ve)
D-Sorbitol Positive (+ve)
Sucrose Positive (+ve)
Tartrate Variable
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Aesculine Hydrolysis Variable
Arginine decarboxylase Positive (+ve)
Esculin Hydrolysis Variable
Lipase Negative (-ve)
Lysine decarboxylases Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Positive (+ve)
Pectate degradation Negative (-ve)
Phenylalanine Deaminase Negative (-ve)
Tryptophan Deaminase Negative (-ve)

Enterobacter cloacae Gram Stain

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

3 thoughts on “Biochemical Test of Enterobacter cloacae”

  1. Hi, my isolate has produce pink mucoid colonies on MAC agar and it shows weakly positive (yellow orange) in methyl red test. Is it possible that these are characteristics of E. cloacae?

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