Biochemical Test of Enterobacter cloacae

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Biochemical Test of Enterobacter cloacae

­Basic Characteristics Properties (Enterobacter cloacae)
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas from glucose Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Positive (+ve)
H2S Negative (-ve)
Indole Negative (-ve)
Motility Motile
MR (Methyl Red) Negative (-ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative Anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Positive (+ve)

Fermentation of
Adonitol Negative (-ve)
Arabinose Positive (+ve)
D-Arabitol Variable
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Meso-Erythritol Negative (-ve)
Glucose Positive (+ve)
Glycerol Variable
Lactose Positive (+ve)
Maltose Positive (+ve)
D-Mannitol Positive (+ve)
D-Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Positive (+ve)
MyoInositol Negative (-ve)
Raffinose Positive (+ve)
Rhamnose Positive (+ve)
Salicin Positive (+ve)
D-Sorbitol Positive (+ve)
Sucrose Positive (+ve)
Tartrate Variable
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions
Acetate Utilization Positive (+ve)
Aesculine Hydrolysis Variable
Arginine decarboxylase Positive (+ve)
Esculin Hydrolysis Variable
Lipase Negative (-ve)
Lysine decarboxylases Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Positive (+ve)
Pectate degradation Negative (-ve)
Phenylalanine Deaminase Negative (-ve)
Tryptophan Deaminase Negative (-ve)

Enterobacter cloacae Gram Stain

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

3 thoughts on “Biochemical Test of Enterobacter cloacae”

  1. Hi, my isolate has produce pink mucoid colonies on MAC agar and it shows weakly positive (yellow orange) in methyl red test. Is it possible that these are characteristics of E. cloacae?

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