Biochemical Test of Yersinia pestis

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Biochemical Test of Yersinia pestis

Basic Characteristics Properties (Yersinia pestis)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Non-Flagellated
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Non-Motile
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of
Adonitol Negative (-ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glucose Positive (+ve)
Glycerol Variable
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Variable
Sorbitol Variable
Sorbose Negative (-ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions
Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Positive (+ve)
Arginine Dehydrolase Negative (-ve)
Lipase Negative (-ve)
Lysine Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

Scanning electron microscope image of Yersinia pestis

Figure: Scanning electron microscope image of Yersinia pestis. Source: www.mirror.co.uk

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

2 thoughts on “Biochemical Test of Yersinia pestis”

  2. Nice information sir.
    Can Yersinia spp. isolated from pus sample of poultry ? Which are the confirmatory tests for Yersinia ?

    Reply

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