Biochemical Test of Yersinia pestis

Biochemical Test of Yersinia pestis

Basic Characteristics Properties (Yersinia pestis)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Non-Flagellated
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Negative (-ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Non-Motile
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glucose Positive (+ve)
Glycerol Variable
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Variable
Sorbitol Variable
Sorbose Negative (-ve)
Sucrose Negative (-ve)
Tartrate Negative (-ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Positive (+ve)
Arginine Dehydrolase Negative (-ve)
Lipase Negative (-ve)
Lysine Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

Scanning electron microscope image of Yersinia pestis

Figure: Scanning electron microscope image of Yersinia pestis. Source: www.mirror.co.uk

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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