Biochemical test of Citrobacter freundii

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Biochemical test of Citrobacter freundii

­Basic Characteristics Properties (Citrobacter freundii)
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas from glucose Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Positive (+ve)
H2S Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative Anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Variable
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glutamate Positive (+ve)
Glycerol Positive (+ve)
Glycogen Positive (+ve)
Inositol Negative (-ve)
Lactose Positive (+ve)
Lactulose Positive (+ve)
Malonate Negative (-ve)
Maltitole Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Positive (+ve)
MyoInositol Positive (+ve)
Palatinose Negative (-ve)
Raffinose Variable
Rhamnose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Sorbose Positive (+ve)
Sucrose Positive (+ve)
Tartrate Negative (-ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Aesculine Hydrolysis Negative (-ve)
Arginine Dehydrolase Variable
Esculin Hydrolysis Negative (-ve)
Lipase Negative (-ve)
Lysine decarboxylase Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine decarboxylase Negative (-ve)
Phenylalanine deaminase Negative (-ve)
Tryptophan deaminase Variable
Tyrosine hydrolysis Positive (+ve)
Biochemical test of Citrobacter freundii
Source: Microbiology In Pictures

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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