Ninhydrin Test- Definition, Principle, Procedure, Result, Uses

Ninhydrin Test

Ninhydrin Test Definition Ninhydrin test is a chemical test performed to detect the presence of ammonia, primary/secondary amines, or amino acids. This test involves the addition of ninhydrin reagent to the test sample that results in the formation of deep blue color, often termed as Ruhemann’s purple, in the presence of an amino group. Objectives of Ninhydrin Test To detect …

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Tollens’ Test- Definition, Principle, Procedure, Result, Uses

Tollens' test

Tollens’ test definition Tollens’ test is a chemical test used to differentiate reducing sugars from non-reducing sugars. This test is also called the silver mirror test based on the end product of this test. Objectives of Tollens’ test To distinguish reducing sugars from non-reducing sugars. To detect the presence of aldehyde containing carbohydrates and differentiate them from ketone containing carbohydrates. …

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Seliwanoff’s Test- Definition, Principle, Procedure, Result, Uses

Seliwanoff’s test definition Seliwanoff’s test is used to differentiate between sugars that have a ketone group (ketose) and sugars that have an aldehyde group (aldoses). This test is a timed color reaction specific to ketohexoses. Objectives of Seliwanoff’s test To detect the presence of ketohexoses in a given sample. To distinguish ketoses from aldoses. Principle of Seliwanoff’s test The reagent …

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Osazone Test- Definition, Principle, Procedure, Result, Uses

Osazone Test- Definition, Principle, Procedure, Result, Uses

Osazone Test Definition Osazone test is a chemical test used to detect reducing sugars. This test even allows the differentiation of different reducing sugars on the basis of the time of appearance of the complex. This test is also termed Phenyl hydrazine test based on the reagent used for this test. Objectives of Osazone Test To detect reducing sugars. To …

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Mucic Acid Test- Definition, Principle, Procedure, Result, Uses

Mucic Acid Test- Definition, Principle, Procedure, Result, Uses

Mucic acid test definition Mucic acid test is a test that is highly specific and is used for the detection of the presence of galactose and lactose. It is also termed galactaric acid that is named after the product of the reaction. Objectives of Mucic acid test To detect the presence of galactose and lactose in a given sample. To …

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Molisch Test- Definition, Principle, Procedure, Result, Uses

Molisch Test- Definition, Principle, Procedure, Result, Uses

Molisch Test Definition Molisch test is a group test for all carbohydrates, either free or bound to proteins or lipids. It is a sensitive test that requires precision for the detection of carbohydrates. Objectives of Molisch Test To detect the presence of carbohydrates in a given sample. To distinguish carbohydrates from other biomolecules. Principle of Molisch Test Image Source: AlexNB. …

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Iodine Test- Definition, Principle, Procedure, Result, Uses

Iodine Test- Definition, Principle, Procedure, Result, Uses

Iodine Test Definition Iodine test is a chemical test used to distinguish mono- or disaccharides from certain polysaccharides like amylase, dextrin, and glycogen. This test has a variation termed starch-iodine test that is performed to indicate the presence of glucose made by plants in the leaves. Objectives of Iodine Test To detect the presence of polysaccharides, primarily starch. Principle of …

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Fehling’s Test- Definition, Principle, Procedure, Result, Uses

Fehling's Test- Definition, Principle, Procedure, Result, Uses

Fehling’s Test Definition Fehling’s test is a chemical test used to differentiate between reducing and non-reducing sugars. This test can also be used to distinguish ketone functional group carbohydrates and water-soluble carbohydrates. Objectives of Fehling’s Test To detect the presence of carbohydrates in a solution. To differentiate between reducing and non-reducing sugars. Principle of Fehling’s Test The carbohydrates having free …

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Bial’s Test- Definition, Principle, Procedure, Result, Uses

Bial’s Test- Definition, Principle, Procedure, Result, Uses

Bial’s Test Definition Bial’s test is a chemical test performed to detect the presence of pentoses and pentosans (derivatives of pentoses). A derivation of this test termed the Bial’s Orchintest is performed to detect the presence of RNA in solutions. Objectives of Bial’s Test To detect the presence of carbohydrates. To distinguish the pentoses and pentosans from other derivatives of …

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Barfoed’s Test- Definition, Principle, Procedure, Result, Uses

Barfoed's Test- Definition, Principle, Procedure, Result, Uses

Barfoed’s Test Definition Barfoed’s test is a chemical test used to detect the presence of monosaccharides which detects reducing monosaccharides in the presence of disaccharides. This reaction can be used for disaccharides, but the reaction would be very slow. Objectives of Barfoed’s Test To detect reducing carbohydrates. To distinguish reducing monosaccharides from disaccharides. Principle of Barfoed’s Test The Barfoed reagent …

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