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Biochemistry

Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.

  • Biochemistry is also termed ‘biological chemistry’ as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.
  • Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.
  • Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.
  • Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.
  • The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.
  • The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.
  • The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.
  • Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.
  • Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.
  • Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.
  • Biochemistry also allows the understanding of nutritional requirements for different living organisms.
  • Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.
  • The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.
  • Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.
  • An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.

Cholesterol Biosynthesis: Site, Pathway, Steps, Regulation, Significance

March 30, 2026March 25, 2026 by Binita Sharma
Cholesterol Biosynthesis

Site and Significance of Cholesterol Biosynthesis The Pathway Breakdown: From Acetyl-CoA to Cholesterol The synthesis of cholesterol may be learnt in 5 stages 1. Formation of HMG-CoA: The Initial Condensation … Read more

Bile Acids: Structure, Properties, Biosynthesis, Functions

March 30, 2026March 24, 2026 by Binita Sharma
Bile Acids

Bile acids are specific and quantitatively important organic components of bile, which are synthesized by hepatocytes from cholesterol.  The bile acids are amphipathic in nature since they possess both polar … Read more

Bile Salts Biosynthesis: Site, Pathways, Steps, Regulations

March 30, 2026March 23, 2026 by Binita Sharma
Bile Salts Biosynthesis

The bile salts are amphipathic steroid-based molecules that are synthesized in the liver using cholesterol; they are the conjugated (glycine/taurine) bile acid products. Biosynthesis starts in hepatocytes through the classical … Read more

Codon Chart: Table, Amino Acids & RNA Wheel Explained

March 30, 2026March 21, 2026 by Sanju Tamang
Codon Chart and Codon Table

Life continues from one generation to the next as a result of replication, transcription, and translation of the genetic code stored in DNA and RNA. The genetic code contains the … Read more

Electron Transport Chain: Equation, Steps, Products, Diagram

March 24, 2026March 19, 2026 by Anupama Sapkota
Electron Transport Chain

The Electron Transport System also called the Electron Transport Chain, is a chain of reactions that converts redox energy available from oxidation of NADH and FADH2, into proton-motive force which … Read more

Glycolysis: 10 Steps, Enzymes, Equation, Regulation, Diagram

March 24, 2026March 18, 2026 by Anupama Sapkota
Glycolysis- definition, equation, enzymes, 10 Steps with diagram

Glycolysis is the central pathway for glucose catabolism in which glucose (6-carbon compound) is converted into pyruvate (3-carbon compound) through a sequence of 10 steps. Image Source: Lehninger Principles of … Read more

Ramachandran Plot: Principle, Tools, Applications, Errors

March 16, 2026March 11, 2026 by Khushi Sharma
Protein Structure

The Ramachandran plot is a fundamental tool in structural biology used to visualize the energetically allowed regions for backbone dihedral angles in a protein. Because protein folding is constrained by … Read more

Gas Chromatography: Principle, Parts, Steps, Procedure, Uses

March 13, 2026March 8, 2026 by Sanju Tamang
Principle of Gas Chromatography

Gas chromatography (GC) is a type of chromatography used to separate and analyze volatile compounds. This separation occurs in an instrument called gas chromatograph that uses an inert gas as … Read more

Flash Chromatography: Principle, Parts, Steps, Examples, Uses

March 13, 2026March 7, 2026 by Sanju Tamang
Flash Chromatography

Flash chromatography is a method used for the rapid separation and purification of chemical compounds in a mixture. It is also known as flash column chromatography or medium-pressure liquid chromatography. … Read more

Fast Protein Liquid Chromatography (FPLC): Principle, Steps, Uses

March 11, 2026March 6, 2026 by Sanju Tamang
Fast Protein Liquid Chromatography (FPLC)

Fast Protein Liquid Chromatography (FPLC) is a liquid chromatography technique used for the separation and purification of proteins and other biomolecules. It was developed in the 1980s by Pharmacia and … Read more

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