Isatin Test- Definition, Objectives, Principle, Procedure, Result, Uses

Isatin Test

Isatin Test Definition Isatin test is a biochemical test for the detection of amino acids like proline and hydroxyproline. The test is a specific test for specific amino acids that produces a colored addition product (also called adduct) with the Isatin reagent. The detection of imino acid proline for a long time had been unsatisfactory as methods like the ninhydrin …

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Hopkin’s Cole Test- Definition, Principle, Procedure, Result, Uses

Hopkin’s Cole Test

Hopkin’s Cole Test Definition Hopkin’s Cole test is a specific test used for the detection of indole ring and thus, tryptophan in proteins. The test is also termed as ‘glyoxylic acid test’ as the reagent contains glyoxylic acid. The test was discovered by Frederick Gowland Hopkins and Sydney W. Cole in 1901 as a part of their work on the …

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Heller’s Test- Definition, Principle, Procedure, Result, Uses

Heller’s Test

Heller’s Test Definition Heller’s test is a biochemical test performed to detect proteins in a sample by the denaturation of those proteins by the addition of strong acids. Heller’s test usually uses concentrated nitric acid for the denaturation of proteins. The test is performed for clinical purposes to detect abnormal proteins in biological fluids, including urine. Heller’s test is a …

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Heat coagulation test of proteins

Heat coagulation test of proteins definition Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of proteins as a response to heat is a common phenomenon. The heat coagulation of proteins occurs in one of the two stages; denaturation and agglutination or the separation of the …

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Esbach Test- Definition, Principle, Procedure, Result, Uses

Esbach test

Esbach test definition Esbach test is one of the oldest biochemical tests used to detect urinary protein like albumin when the urine is combined with citric and picric acid. Esbach test, even though not discussed much through the history, is useful for both qualitative and quantitative determination of albumin in the urine sample. The test was discovered by Georges Hubert …

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Ehrlich Test- Definition, Principle, Procedure, Result, Uses

Ehrlich Test

Ehrlich Test Definition Ehrlich Test is a biochemical test used for the detection of amino acid, tryptophan, in a protein sample. Ehrlich test is also termed the p-dimethylamino benzaldehyde test, named after the Ehrlich reagent. The test is named after the Nobel Prize winner Paul Ehrlich, who identified this test during the distinction of typhoid from simple diarrhea. It is …

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Acree-Rosenheim Test- Definition, Principle, Procedure, Result, Uses

Acree-Rosenheim test

Acree-Rosenheim test definition Acree-Rosenheim Test is a biochemical test used for the detection of tryptophan molecules in a protein sample. Acree-Rosenheim test is one of the essential tests for the confirmation of formaldehyde in the milk as milk vendors use formaldehyde as a preservative. This test thus helps to understand the condition of the milk sample so as to determine …

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Ninhydrin Test- Definition, Principle, Procedure, Result, Uses

Ninhydrin Test

Ninhydrin Test Definition Ninhydrin test is a chemical test performed to detect the presence of ammonia, primary/secondary amines, or amino acids. This test involves the addition of ninhydrin reagent to the test sample that results in the formation of deep blue color, often termed as Ruhemann’s purple, in the presence of an amino group. Objectives of Ninhydrin Test To detect …

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Tollens’ Test- Definition, Principle, Procedure, Result, Uses

Tollens' test

Tollens’ test definition Tollens’ test is a chemical test used to differentiate reducing sugars from non-reducing sugars. This test is also called the silver mirror test based on the end product of this test. Objectives of Tollens’ test To distinguish reducing sugars from non-reducing sugars. To detect the presence of aldehyde containing carbohydrates and differentiate them from ketone containing carbohydrates. …

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Seliwanoff’s Test- Definition, Principle, Procedure, Result, Uses

Seliwanoff’s test definition Seliwanoff’s test is used to differentiate between sugars that have a ketone group (ketose) and sugars that have an aldehyde group (aldoses). This test is a timed color reaction specific to ketohexoses. Objectives of Seliwanoff’s test To detect the presence of ketohexoses in a given sample. To distinguish ketoses from aldoses. Principle of Seliwanoff’s test The reagent …

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