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Biochemistry

Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.

  • Biochemistry is also termed ‘biological chemistry’ as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.
  • Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.
  • Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.
  • Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.
  • The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.
  • The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.
  • The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.
  • Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.
  • Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.
  • Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.
  • Biochemistry also allows the understanding of nutritional requirements for different living organisms.
  • Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.
  • The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.
  • Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.
  • An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.

Molisch Test- Definition, Principle, Procedure, Result, Uses

September 17, 2022May 19, 2022 by Anupama Sapkota
Molisch Test- Definition, Principle, Procedure, Result, Uses

Molisch test is a group test for all carbohydrates, either free or bound to proteins or lipids. It is a sensitive test that requires precision for the detection of carbohydrates. … Read more

Primary vs Secondary Metabolites- Definition, 12 Differences, Examples

October 21, 2022May 18, 2022 by Anupama Sapkota
Differences between Primary Metabolites & Secondary Metabolites

Primary Metabolites Definition Primary metabolites are the compounds that are directly involved in the metabolic pathways of an organism necessary for its growth, development, and reproduction. These metabolites are associated … Read more

Microbial degradation of lignin (Enzymes, Steps, Mechanisms)

September 5, 2022May 17, 2022 by Anupama Sapkota
Microbial degradation of lignin

What is lignin? Lignin is a group of complex aromatic polymers resulting from the oxidative combination of 4-hydroxyphenylpropanoids. Lignin is a phenolic polymer that is amorphous, irregular three-dimensional, and high … Read more

Microbial degradation of cellulose (Enzymes, Steps, Mechanisms)

September 15, 2022May 13, 2022 by Anupama Sapkota
Microbial degradation of cellulose (Enzymes, Steps, Mechanisms)

What is cellulose? Cellulose is an organic polysaccharide composed of a linear chain of hundreds of β-linked D-glucose units. Figure: Cellulose, a linear polymer of D-glucose units (two are shown) … Read more

Protein Structure- Primary, Secondary, Tertiary, and Quaternary

December 29, 2022May 6, 2022 by Sagar Aryal, PhD
Protein Structure- Primary, Secondary, Tertiary and Quaternary

Four levels of hierarchy in protein conformation can be described. A. PRIMARY STRUCTURE PRIMARY STRUCTURE refers to the order of the amino acids in the peptide chain.1. The free α-amino … Read more

Esbach Test- Definition, Principle, Procedure, Result, Uses

September 5, 2022April 23, 2022 by Anupama Sapkota
Esbach test

Esbach test definition Esbach test is one of the oldest biochemical tests used to detect urinary protein like albumin when the urine is combined with citric and picric acid. Esbach … Read more

Acree-Rosenheim Test- Definition, Principle, Procedure, Result, Uses

September 5, 2022April 20, 2022 by Anupama Sapkota
Acree-Rosenheim test

Acree-Rosenheim test definition Acree-Rosenheim Test is a biochemical test used for the detection of tryptophan molecules in a protein sample. Acree-Rosenheim test is one of the essential tests for the … Read more

Fehling’s Test- Definition, Principle, Procedure, Result, Uses

September 5, 2022April 18, 2022 by Anupama Sapkota
Fehling's Test- Definition, Principle, Procedure, Result, Uses

Fehling’s Test Definition Fehling’s test is a chemical test used to differentiate between reducing and non-reducing sugars. This test can also be used to distinguish ketone functional group carbohydrates and … Read more

Bial’s Test- Definition, Principle, Procedure, Result, Uses

September 5, 2022April 18, 2022 by Anupama Sapkota
Bial’s Test- Definition, Principle, Procedure, Result, Uses

Bial’s Test Definition Bial’s test is a chemical test performed to detect the presence of pentoses and pentosans (derivatives of pentoses). A derivation of this test termed the Bial’s Orchintest … Read more

Barfoed’s Test- Definition, Principle, Procedure, Result, Uses

September 5, 2022April 18, 2022 by Anupama Sapkota
Barfoed's Test- Definition, Principle, Procedure, Result, Uses

Barfoed’s Test Definition Barfoed’s test is a chemical test used to detect the presence of monosaccharides which detects reducing monosaccharides in the presence of disaccharides. This reaction can be used … Read more

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