The Michaelis–Menten Model

The Michaelis–Menten model

Enzyme velocity Substrate and enzyme concentration The Michaelis–Menten model The Michaelis–Menten equation: where,  Km = (k2 + k3)/k1 and Vmax is the maximum velocity. The Michaelis constant, Km, is equal … Read more

Lineweaver–Burk Plot

Lineweaver–Burk Plot

Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from a hyperbolic plot. Because of this difficulty, the Michaelis–Menten equation was transformed … Read more