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Basic Microbiology

Microbiology is the branch of science that deals with microscopic organisms and their interaction with other microscopic and macroscopic organisms.

  • Microorganisms are tiny microscopic organisms that are too small to be seen with naked eyes and thus, can only be seen with a microscope. Microorganisms include microscopic organisms like bacteria, fungi, archaea, protozoa, and viruses.
  • Basic microscopy deals with a diverse group of studies that help in research related to the biochemistry, physiology, cell biology, ecology, evolution, and clinical aspects of microorganisms, including the host response to these agents.
  • Microbiology also deals with the structure, function, classification of such organisms, along with exploiting and controlling their activities.
  • The concept of microbiology began with the discovery of the microscope by Anton Von Leeuwenhoek.
  • On the one hand, microbes are used for their unique features which allow the production of antibiotics, amino acids, hormones, and other therapeutic compounds, and also the production of food and food-related products.
  • Microorganisms are also involved in the decomposition of components such as lignocellulosic biomass for second-generation ethanol or biogas.
  • Similarly, certain genetic features and biochemical abilities of microorganisms make them dangerous for industry (food spoilage) as well as human health.
  • Microbiology, initially, was only associated with pathogenic microorganisms that result in different forms of diseases in different groups of living beings.
  • With the establishment of microbiology as a discipline, the application of microorganisms in different areas has also increased.
  • The use of microorganisms in food and pharmaceuticals has given rise to the branching of microbiology into further disciplines and studies.
  • Thus, over the years, the branch has been classified into further groups like agriculture microbiology, food microbiology, pharmaceutical microbiology, systemic microbiology, etc.
  • The studies in microbiology have also increased due to the use of microorganisms in different researches as these are easy to manipulate and reproduce when compared to other living organisms.
  • Studies in microbiology are essential for the discovery of new and advanced methods for the discovery of emerging microorganisms and associated diseases and applications.
  • Microbiology also deals with techniques for the identification of these microorganisms, their classification, and the life cycle.
  • All of this allows for a better understanding of microorganisms and their role in maintaining the ecosystem.
  • Microbiology and microorganisms can be applied for the formation of new genetically engineered microorganisms by a process like genetic recombination.
  • Besides, different microorganisms have found their application in the production of food, industrial products, and antibiotics.

Bacteria vs. Virus: 28 Differences with Examples

June 6, 2023 by Sagar Aryal
Differences Between Bacteria and Virus

Some of the differences between bacteria and virus are as follows: S.N. Character Bacteria Virus 1. Cell type Prokaryotic cells Acellular 2. Number of cells Single-celled No cell 3. Size … Read more

Prokaryotes vs.ย Eukaryotes (47 Key Differences)

January 3, 2024June 2, 2023 by Anupama Sapkota
Prokaryotes vs Eukaryotes

S.N. Character Prokaryotes Eukaryotes 1. Term Origin Greek for โ€œprimitive nucleusโ€ Greek for โ€œtrue nucleusโ€ 2. Definition Organisms are made up of cell(s) that lack a cell nucleus or any … Read more

Pour Plate Method- Definition, Principle, Procedure, Uses

April 3, 2025August 27, 2022 by Prashant Dahal
Pour Plate Method

The pour Plate Method technique was established in the laboratory of Robert Koch and is still being used widely since his period. This method is suitable for facultative, Microaerophilic, and … Read more

Spread Plate Method- Definition, Principle, Procedure, Uses

April 3, 2025August 26, 2022 by Prashant Dahal
Spread Plate Method

Spread Plate Method is one of the widely used culture techniques in microbiology laboratories due to its ease and simplicity. This method is suitable for aerobic and facultative aerobic microorganisms. … Read more

Isolation of Bacillus thuringiensis (Bt) from Soil sample

September 5, 2022July 5, 2022 by Sagar Aryal
Isolation of Bacillus thuringiensis (Bt) from Soil sample

Bacillus thuringiensisย (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip … Read more

Biosafety Levels (BSL-1, BSL-2, BSL-3 and BSL-4)

October 21, 2022June 16, 2022 by Anupama Sapkota
Biosafety Levels

Biosafety is the prevention of risk to human health and safety, and the conservation of the environment and the pathogen, as a result of the use for research and commerce … Read more

Kochโ€™s postulates and its limitations

September 5, 2022June 10, 2022 by Sagar Aryal
Kochโ€™s postulates and its limitations

Kochโ€™s postulate forms the very basis of the pathogenic microbiology. The causality of almost all infectious diseases is based on the postulate and theories developed by Robert Koch, who is … Read more

Biochemical Test and Identification of Bacteria

January 15, 2023June 10, 2022 by Sagar Aryal
Biochemical Test of Bacteria

Biochemical reactions are significant in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, … Read more

Isolation of Actinomycetes from Soil sample

September 9, 2023June 6, 2022 by Sagar Aryal
Procedure ofย Isolation of Actinomycetes

Figure: Free living actinomycetes are ubiquitous in soil environments as well as in marine and fresh water ecosystems. In addition, they have an important ecological role in the turnover of organic … Read more

Biosafety Cabinets- Definition, Classes (I, II, III) and Types

September 5, 2022June 2, 2022 by Anupama Sapkota
Biosafety Cabinets

Biosafety Cabinets Definition Biosafety Cabinets (BSCs) are enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes. The primary purpose of biosafety cabinets is … Read more

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