Salt Tolerance Test

Salt Tolerance Test

Salt Tolerance Test Objectives To determine the ability of an organism to grow in high concentrations of salt. It is used for the differentiation of Enterococci from Non-Enterococci. Principle Salt acts as a selective agent and interferes with membrane permeability and osmotic equilibrium. The salt tolerance medium is a selective and differential medium in which … Read more Salt Tolerance Test

Kligler Iron Agar Test

Kligler Iron Agar Test

Kligler Iron Agar Test Objective To differentiate the enteric gram-negative bacilli based on the production of hydrogen sulfide and fermentation of dextrose and lactose. Principle Kligler Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae by demonstrating hydrogen sulfide production and the fermentation of dextrose and lactose. Kligler agar used for … Read more Kligler Iron Agar Test

Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia a: Burkholderia cepacia ATCC (Image Source: ATCC) b: Burkholderia cepacia (Image Source: Rapid Microbiology) c: Burkholderia cepacia (Image Source: OMICS International) d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific) e: Scanning Electron Micrograph of Burkholderia cepacia (Image Source: Wikipedia) f: Burkholderia cepacia … Read more Biochemical Test of Burkholderia cepacia

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis Image: Granuloma inguinale (donovanosis) in a conventional Pap test. A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes. B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b). C: Macrophages show numerous safety pin-shaped structures … Read more Biochemical Test of Klebsiella granulomatis

Biochemical Test of Haemophilus parainfluenzae

Biochemical Test of Haemophilus parainfluenzae

Biochemical Test of Haemophilus parainfluenzae Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus parainfluenzae) Capsule Variable Catalase Variable Gas Variable Gram Staining Gram-negative (-ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Variable Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Positive (+ve) Oxidase Positive (+ve) Shape Small, pleomorphic rods, usually with … Read more Biochemical Test of Haemophilus parainfluenzae

Biochemical Test of Francisella tularensis subsp. tularensis

Biochemical Test of Francisella tularensis

Biochemical Test of Francisella tularensis subsp. tularensis Image Source: New York State Health Department Some of the characteristics are as follows: Basic Characteristics Properties (Francisella tularensis subsp. tularensis) Capsule Positive (+ve) Catalase Positive (+ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Faintly stained Gram-negative H2S (in cysteine-supplemented medium) Positive (+ve) … Read more Biochemical Test of Francisella tularensis subsp. tularensis

Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei Image Source: University of Cambridge Some of the characteristics are as follows: Basic Characteristics Properties (Burkholderia pseudomallei) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-negative (-ve) H2S Negative (-ve) Indole Negative (-ve) Motility Positive (+ve) Nitrate … Read more Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Enterobacter aerogenes

Biochemical Test of Enterobacter aerogenes

Biochemical Test of Enterobacter aerogenes Image Source: MicrobeWiki and Getty Images. Some of the characteristics are as follows: Basic Characteristics Properties (Enterobacter aerogenes) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative H2S Negative (-ve) Indole Negative (-ve) Motility Positive (+ve) MR … Read more Biochemical Test of Enterobacter aerogenes

Biochemical Test of Clostridium botulinum

Biochemical Test of Clostridium botulinum

Biochemical Test of Clostridium botulinum (Type A and proteolytic strains of types B and F) Basic Characteristics Properties (Clostridium botulinum) Ammonia Production Positive (+ve) 20% Bile Negative (-ve) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Peritrichous Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-positive H2S Positive (+ve) Hemolysis Positive (+ve). β-hemolytic Indole Negative (-ve) Milk Digestion … Read more Biochemical Test of Clostridium botulinum

Biochemical Test of Enterococcus faecalis

Biochemical Test of Enterococcus faecalis

Biochemical Test of Enterococcus faecalis Basic Characteristics Properties (Enterococcus faecalis ) Bile Resistance Resistance to 40% (v/v) bile Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Variable Gram Staining Gram-positive H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Negative (-ve) 6.5% NaCl Positive (+ve) OF (Oxidative-Fermentative) Fermentative … Read more Biochemical Test of Enterococcus faecalis