Pyruvate Broth Test

Pyruvate Broth Test

Objectives of Pyruvate Broth Test To determine the ability of an organism to utilize pyruvate. It aids in the differentiation between Enterococcus faecalis and Enterococcus faecium. Principle of Pyruvate Broth Test In the identification of microorganisms, the widely used method is the … Read more

Sulfur Reduction Test

Sulfur Reduction Test

Objectives To determine the ability of the organism to reduce sulfur for the production of hydrogen sulfide. To differentiate members of Enterobacteriaceae, especially the sulfur-reducing Salmonella and Proteus from the non-reducing Morganella morganii and Providencia rettgeri. Principle The Sulfur Reduction … Read more

Salt Tolerance Test

Salt Tolerance Test

Objectives To determine the ability of an organism to grow in high concentrations of salt. It is used for the differentiation of Enterococci from Non-Enterococci. Principle Salt acts as a selective agent and interferes with membrane permeability and osmotic equilibrium. … Read more

Kligler Iron Agar Test

Kligler Iron Agar Test

Objective To differentiate the enteric gram-negative bacilli based on the production of hydrogen sulfide and fermentation of dextrose and lactose. Principle Kligler Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae by demonstrating hydrogen sulfide production … Read more

Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia

a: Burkholderia cepacia ATCC (Image Source: ATCC) b: Burkholderia cepacia (Image Source: Rapid Microbiology) c: Burkholderia cepacia (Image Source: OMICS International) d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific) e: Scanning Electron … Read more

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Image: Granuloma inguinale (donovanosis) in a conventional Pap test. A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes. B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and … Read more

Biochemical Test of Haemophilus parainfluenzae

Biochemical Test of Haemophilus parainfluenzae

Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus parainfluenzae) Capsule Variable Catalase Variable Gas Variable Gram Staining Gram-negative (-ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Variable Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Positive … Read more

Biochemical Test of Francisella tularensis subsp. tularensis

Biochemical Test of Francisella tularensis

Image Source: New York State Health Department Some of the characteristics are as follows: Basic Characteristics Properties (Francisella tularensis subsp. tularensis) Capsule Positive (+ve) Catalase Positive (+ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Faintly … Read more

Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge Some of the characteristics are as follows: Basic Characteristics Properties (Burkholderia pseudomallei) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-negative (-ve) … Read more

Biochemical Test of Enterobacter aerogenes

Biochemical Test of Enterobacter aerogenes

Image Source: MicrobeWiki and Getty Images. Some of the characteristics are as follows: Basic Characteristics Properties (Enterobacter aerogenes) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative … Read more

Biochemical Test of Clostridium botulinum

Biochemical Test of Clostridium botulinum

(Type A and proteolytic strains of types B and F) Basic Characteristics Properties (Clostridium botulinum) Ammonia Production Positive (+ve) 20% Bile Negative (-ve) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Peritrichous Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-positive H2S Positive … Read more

Biochemical Test of Enterococcus faecalis

Biochemical Test of Enterococcus faecalis

Basic Characteristics Properties (Enterococcus faecalis ) Bile Resistance Resistance to 40% (v/v) bile Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Variable Gram Staining Gram-positive H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) … Read more

Biochemical Test of Hafnia alvei

Biochemical Test of Hafnia alvei Basic Characteristics Properties (Hafnia alvei) Capsule Non-Capsulated Catalase Positive (+ve) Citrate Positive (+ve) Flagella Flagellated Gas from Glucose Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Positive (+ve) H2S Negative … Read more

Biochemical Test of Escherichia coli (E. coli)

Biochemical Test of Escherichia coli (E. coli)

Biochemical Test of Escherichia coli (E. coli) Basic Characteristics Properties (E. coli) Capsule Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve) Flagella Flagellated Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Negative … Read more

Biochemical Test of Enterobacter cloacae

Biochemical Test of Enterobacter cloacae ­Basic Characteristics Properties (Enterobacter cloacae) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas from glucose Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Positive (+ve) … Read more

Biochemical Test of Bacillus anthracis

Biochemical Test of Bacillus anthracis

Biochemical Test of Bacillus anthracis ­Basic Characteristics Properties (Bacillus anthracis) Capsule Capsulated Catalase Positive (+ve) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) Hemolysis Negative (-ve) Indole Positive (+ve) Motility Negative (-ve) Nitrate Reduction Positive (+ve) Oxidase … Read more

Biochemical Test of Staphylococcus epidermidis

Biochemical Test of Staphylococcus epidermidis

Biochemical Test of Staphylococcus epidermidis ­Basic Characteristics Properties (Staphylococcus epidermidis) Capsule Mostly Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Motility … Read more

Biochemical Test of Serratia marcescens

Biochemical Test of Serratia marcescens

Biochemical Test of Serratia marcescens ­Basic Characteristics Properties (Serratia marcescens) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Variable Gelatin Hydrolysis Positive (+ve) Gram Staining Negative (-ve) H2S Negative (-ve) Indole Negative (-ve) Motility Positive … Read more

Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile ­Basic Characteristics Properties (Clostridium difficile) Capsule Capsulated Catalase Negative (-ve) Flagella Flagellated Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Motile Nitrate Reduction Negative (-ve) … Read more

Biochemical Test of Vibrio cholerae

Biochemical Test of Vibrio cholerae

Image Source: Belén García, dibujandoafrica.com ­Basic Characteristics Properties (Vibrio cholerae) Capsule Non-Capsulated Citrate +ve Flagella Flagellated Gas -ve Gelatin Hydrolysis +ve Gram Staining -ve H2S -ve Hemolysis Beta Hemolysis Indole +ve Motility Motile MR (Methyl Red) -ve Nitrate Reduction +ve … Read more