Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia

a: Burkholderia cepacia ATCC (Image Source: ATCC)

b: Burkholderia cepacia (Image Source: Rapid Microbiology)

c: Burkholderia cepacia (Image Source: OMICS International)

d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific)

e: Scanning Electron Micrograph of Burkholderia cepacia (Image Source: Wikipedia)

f: Burkholderia cepacia gram stain (Image Source: DOI: 10.1099/jmmcr.0.005081)

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Some of the characteristics are as follows:

Basic Characteristics Properties (Burkholderia cepacia)
Bacteriocin Positive (+ve)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-stain-negative
Hemolysis Negative (-ve)
Motility Positive(+ve)
Nitrate Reduction Negative (-ve)
Oxidase Variable
Pigment Positive (+ve)
Shape                   Straight or curved rods
Spore Negative (-ve)
Urease Positive (+ve)

Fermentation of

Adonitol Positive (+ve)
Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Arabitol Positive (+ve)
Arbutin Positive (+ve)
Cellobiose Variable
Dulcitol Positive (+ve)
Erythritol Negative (-ve)
Fucose Positive (+ve)
Glucosamine Positive (+ve)
2-Ketogluconate Positive (+ve)
5-Ketogluconate Positive (+ve)
Lactose Negative (-ve)
Malonate Positive (+ve)
Maltose Variable
Melibiose Variable
Mucate Positive (+ve)
N-Acetylglucosamine Positive (+ve)
Raffinose Variable
Rhamnose Variable
Ribose Positive (+ve)
Salicin Positive (+ve)
Starch Negative (-ve)
Sucrose Variable
Tagatose Positive (+ve)
Tartrate Positive (+ve)
Trehalose Negative (-ve)
Xylose Variable

Enzymatic Reactions

Arginine Dihydrolase Negative (-ve)
Beta Lactamase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Variable
Phospholipase C Positive (+ve)
Protease Positive (+ve)

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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