Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia

a: Burkholderia cepacia ATCC (Image Source: ATCC)

b: Burkholderia cepacia (Image Source: Rapid Microbiology)

c: Burkholderia cepacia (Image Source: OMICS International)

d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific)

e: Scanning Electron Micrograph of Burkholderia cepacia (Image Source: Wikipedia)

f: Burkholderia cepacia gram stain (Image Source: DOI: 10.1099/jmmcr.0.005081)

Some of the characteristics are as follows:

Basic Characteristics Properties (Burkholderia cepacia)
Bacteriocin Positive (+ve)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gelatin Hydrolysis Variable
Gram Staining Gram-stain-negative
Hemolysis Negative (-ve)
Motility Positive(+ve)
Nitrate Reduction Negative (-ve)
Oxidase Variable
Pigment Positive (+ve)
Shape                   Straight or curved rods
Spore Negative (-ve)
Urease Positive (+ve)

Fermentation of

Adonitol Positive (+ve)
Amygdalin Positive (+ve)
Arabinose Positive (+ve)
Arabitol Positive (+ve)
Arbutin Positive (+ve)
Cellobiose Variable
Dulcitol Positive (+ve)
Erythritol Negative (-ve)
Fucose Positive (+ve)
Glucosamine Positive (+ve)
2-Ketogluconate Positive (+ve)
5-Ketogluconate Positive (+ve)
Lactose Negative (-ve)
Malonate Positive (+ve)
Maltose Variable
Melibiose Variable
Mucate Positive (+ve)
N-Acetylglucosamine Positive (+ve)
Raffinose Variable
Rhamnose Variable
Ribose Positive (+ve)
Salicin Positive (+ve)
Starch Negative (-ve)
Sucrose Variable
Tagatose Positive (+ve)
Tartrate Positive (+ve)
Trehalose Negative (-ve)
Xylose Variable

Enzymatic Reactions

Arginine Dihydrolase Negative (-ve)
Beta Lactamase Positive (+ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine Decarboxylase Variable
Phospholipase C Positive (+ve)
Protease Positive (+ve)

About Author

Photo of author

Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.