Biochemical Test of Burkholderia pseudomallei

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Last Updated on February 4, 2021 by Sagar Aryal

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Burkholderia pseudomallei)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-negative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRod-shaped; “safety pin” appearance (bipolar staining)
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)K/NC/H2S -ve
UreaseNegative (-ve)

Fermentation of

ArabinosePositive (+ve)
ArabitolPositive (+ve)
CellobiosePositive (+ve)
DulcitolPositive (+ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
HippuratePositive (+ve)
InositolPositive (+ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)

Enzymatic Reactions

Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
LecithinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylasePositive (+ve)
Superoxide DismutasePositive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria
  3. Prakash, A., Thavaselvam, D., Kumar, A., Kumar, A., Arora, S., Tiwari, S., Barua, A., Sathyaseelan, K. (2014). Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India. SpringerPlus3, 438. doi:10.1186/2193-1801-3-438

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