Biochemical Test of Burkholderia pseudomallei

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Last Updated on January 6, 2020 by Sagar Aryal

Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Burkholderia pseudomallei)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-negative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRod-shaped; “safety pin” appearance (bipolar staining)
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)K/NC/H2S -ve
UreaseNegative (-ve)

Fermentation of

ArabinosePositive (+ve)
ArabitolPositive (+ve)
CellobiosePositive (+ve)
DulcitolPositive (+ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
HippuratePositive (+ve)
InositolPositive (+ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolVariable
MannosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)

Enzymatic Reactions

Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
LecithinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylasePositive (+ve)
Superoxide DismutasePositive (+ve)

References

  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria
  2. https://www.sciencedirect.com/topics/medicine-and-dentistry/burkholderia-pseudomallei
  3. Prakash, A., Thavaselvam, D., Kumar, A., Kumar, A., Arora, S., Tiwari, S., Barua, A., Sathyaseelan, K. (2014). Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India. SpringerPlus3, 438. doi:10.1186/2193-1801-3-438
  4. https://www.researchgate.net/publication/6459185_Mycobacterium_avium_and_Burkholderia_pseudomallei_Melioidosis_coinfection_in_an_HIV-positive_patient
  5. http://medind.nic.in/iau/t03/i2/iaut03i2p129.pdf

Biochemical Test of Burkholderia pseudomallei

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