Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge

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Some of the characteristics are as follows:

Basic CharacteristicsProperties (Burkholderia pseudomallei)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitrateMostly Negative (-ve)
FlagellaPositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-negative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRod-shaped; “safety pin” appearance (bipolar staining)
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)K/NC/H2S -ve
UreaseNegative (-ve)
Fermentation of
ArabinosePositive (+ve)
ArabitolPositive (+ve)
CellobiosePositive (+ve)
DulcitolPositive (+ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
HippuratePositive (+ve)
InositolPositive (+ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Enzymatic Reactions
Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
LecithinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylasePositive (+ve)
Superoxide DismutasePositive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria
  3. Prakash, A., Thavaselvam, D., Kumar, A., Kumar, A., Arora, S., Tiwari, S., Barua, A., Sathyaseelan, K. (2014). Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India. SpringerPlus3, 438. doi:10.1186/2193-1801-3-438

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

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