Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge

Some of the characteristics are as follows:

Basic Characteristics Properties (Burkholderia pseudomallei)
Capsule Positive (+ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas Negative (-ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Gram-negative (-ve)
H2S Negative (-ve)
Indole Negative (-ve)
Motility Positive (+ve)
Nitrate Reduction Positive (+ve)
Oxidase Positive (+ve)
Pigment Negative (-ve)
Shape Rod-shaped; “safety pin” appearance (bipolar staining)
Spore Negative (-ve)
TSIA (Triple Sugar Iron Agar) K/NC/H2S -ve
Urease Negative (-ve)

Fermentation of

Arabinose Positive (+ve)
Arabitol Positive (+ve)
Cellobiose Positive (+ve)
Dulcitol Positive (+ve)
Erythritol Positive (+ve)
Fructose Positive (+ve)
Galactose Positive (+ve)
Glucose Positive (+ve)
Glycerol Positive (+ve)
Hippurate Positive (+ve)
Inositol Positive (+ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Variable
Mannose Positive (+ve)
Rhamnose Negative (-ve)
Ribose Positive (+ve)
Starch Positive (+ve)
Sucrose Positive (+ve)
Tartrate Negative (-ve)
Trehalose Positive (+ve)
Xylose Negative (-ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Arginine Dehydrolase Positive (+ve)
Esculin Hydrolysis Variable
Lecithinase Positive (+ve)
Lipase Positive (+ve)
Ornithine Decarboxylase Positive (+ve)
Superoxide Dismutase Positive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria
  3. Prakash, A., Thavaselvam, D., Kumar, A., Kumar, A., Arora, S., Tiwari, S., Barua, A., Sathyaseelan, K. (2014). Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India. SpringerPlus3, 438. doi:10.1186/2193-1801-3-438

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He attended St. Xavier’s College, Maitighar, Kathmandu, Nepal, to complete his Master of Science in Microbiology. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from Feb 2015 to June 2019. After teaching microbiology for more than four years, he joined the Central Department of Microbiology, Tribhuvan University, to pursue his Ph.D. in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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