Biochemical Test of Burkholderia pseudomallei

Biochemical Test of Burkholderia pseudomallei

Image Source: University of Cambridge

Some of the characteristics are as follows:

Basic CharacteristicsProperties (Burkholderia pseudomallei)
CapsulePositive (+ve)
CatalasePositive (+ve)
CitrateMostly Negative (-ve)
FlagellaPositive (+ve)
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram-negative (-ve)
H2SNegative (-ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
Nitrate ReductionPositive (+ve)
OxidasePositive (+ve)
PigmentNegative (-ve)
ShapeRod-shaped; “safety pin” appearance (bipolar staining)
SporeNegative (-ve)
TSIA (Triple Sugar Iron Agar)K/NC/H2S -ve
UreaseNegative (-ve)
Fermentation of
ArabinosePositive (+ve)
ArabitolPositive (+ve)
CellobiosePositive (+ve)
DulcitolPositive (+ve)
ErythritolPositive (+ve)
FructosePositive (+ve)
GalactosePositive (+ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
HippuratePositive (+ve)
InositolPositive (+ve)
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TartrateNegative (-ve)
TrehalosePositive (+ve)
XyloseNegative (-ve)
Enzymatic Reactions
Acetate UtilizationPositive (+ve)
Arginine DehydrolasePositive (+ve)
Esculin HydrolysisVariable
LecithinasePositive (+ve)
LipasePositive (+ve)
Ornithine DecarboxylasePositive (+ve)
Superoxide DismutasePositive (+ve)


  1. Bergey’s Manual of Systematic Bacteriology Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria
  3. Prakash, A., Thavaselvam, D., Kumar, A., Kumar, A., Arora, S., Tiwari, S., Barua, A., Sathyaseelan, K. (2014). Isolation, identification and characterization of Burkholderia pseudomallei from soil of coastal region of India. SpringerPlus3, 438. doi:10.1186/2193-1801-3-438

About Author

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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is currently doing his Ph.D. from the Central Department of Microbiology, Tribhuvan University in collaboration with Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He did his M.Sc. in Microbiology and B.Sc. in Microbiology from St. Xavier’s College, Kathmandu, Nepal. He worked as a Lecturer at St. Xavier’s College, Maitighar, Kathmandu, Nepal, from March 2017 to June 2019. He is interested in research on actinobacteria, myxobacteria, and natural products. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers.

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