Culture media used in Microbiology with their uses 4.67/5 (36)

Culture media used in Microbiology with their uses

A7 and A8 agars

A7 and A8 agars are selective and differential media used for the cultivation, identification, and differentiation of Ureaplasma spp. and Mycoplasma hominis.

 

Alkaline peptone water

Alkaline peptone water is an enrichment broth used for the isolation of small numbers of Vibrio and Aeromonas organisms from stool specimens.

 

American Trudeau Society medium

American Trudeau Society medium is a nonselective enriched medium used for the isolation and cultivation of mycobacteria.

Culture media used in Microbiology with their uses


Amies transport medium with and without charcoal

Amies transport medium is a modification of Stuart’s medium. Amies medium with charcoal is preferred for the isolation of Neisseria spp.

 

Anaerobic blood agar (CDC)

The Centers for Disease Control and Prevention (CDC) formulation of anaerobic blood agar is a general-purpose medium used for the isolation and cultivation of anaerobic bacteria.

 

10B arginine broth; Shepard’s broth

10B arginine broth is a medium used for the transport and growth of M. hominis and Ureaplasma urealyticum.

 

Ashdown agar

Ashdown agar is a selective and differential medium for the isolation of Pseudomonas pseudomallei.

 

Bacillus cereus medium

B. cereus medium is an enriched medium used for the isolation of B. cereus.

 

BACTEC 12B radiometric medium

BACTEC 12B medium (BD Biosciences) is a liquid nonselective medium used for the isolation and identification of Mycobacterium species in conjunction with the BACTEC system

 

Bacteroides bile esculin agar

Bacteroides bile esculin agar is an enriched, selective, and differential medium used for the isolation and presumptive identification of members of the Bacteroides fragilis group and Bilophila wadsworthia.

 

Baird-Parker agar base

Baird-Parker agar base is an enriched, selective, and differential agar used for the detection of coagulase-positive staphylococci (S. aureus) from food and other nonclinical sources.

 

Barbour-Stoenner-Kelly medium (Sigma-Aldrich Co., St. Louis, Mo.)

Barbour-Stoenner-Kelly medium is a complicated medium for the isolation of Borrelia burgdorferi.

 

Bile esculin agar

Bile esculin agar is a selective and differential medium used for the isolation and differentiation of Enterococcus and Streptococcus bovis (group D Streptococcus) from non-group D Streptococcus.

 

Bile esculin agar plus vancomycin at 6 μg/ml

Bile esculin agar plus vancomycin is a selective and differential medium used to identify vancomycin-resistant streptococci and enterococci.

 

Bile esculin azide agar and broth (Enterococcosel)

Bile esculin azide agar or broth is a selective and differential medium for S. bovis (group D streptococcus) and enterococci.

 

Bismuth sulfite agar

Bismuth sulfite agar is a highly selective and differential medium used for the isolation of Salmonella enterica serovar Typhi and other enteric bacilli.

 

Blood agar

It is same as Columbia agar with 5% sheep blood.

 

Blood culture media

All blood culture medium formulations are based on a nutrient peptone broth with variations due to hydrolysis or digestion of the source protein.

 

Bordet-Gengou medium

Bordet-Gengou medium is an enriched medium used for the isolation and cultivation of Bordetella pertussis from clinical specimens.

 

Brain heart infusion agar

Brain heart infusion agar is a general-purpose medium used for the isolation of a wide variety of pathogens, including yeasts, molds, and bacteria.

 

Brain heart infusion agar with 7% horse blood and brain heart infusion agar with 1% serum

Brain heart infusion agar with horse blood or serum enriches the medium for isolation of Helicobacter spp.

 

Brain heart infusion broth

Brain heart infusion broth is a general-purpose clear liquid medium that is used to cultivate a wide variety of organisms. Formulations with 6.5% NaCl are used for the isolation of salt-tolerant streptococci, formulations with 0.1% agar that reduce O2 tension favor anaerobes, and formulations with Fildes enrichment are used for the isolation of fastidious organisms such as Haemophilus and Neisseria.

 

Brain heart infusion-vancomycin agar

Brain heart infusion-vancomycin agar is a selective medium used for the isolation of vancomycin-resistant enterococci. The base is brain heart infusion agar. Vancomycin (6g/ml) is added to select for vancomycin-resistant enterococci.

 

Brilliant green agar

Brilliant green agar is a highly selective and differential medium used for the isolation of Salmonella species except for serovar Typhi.

 

Brucella agar

Brucella agar is a medium designed originally for the purpose of isolating Brucella spp. from dairy products.

 

Brucella agar with cefoxitin and cycloserine

Brucella agar with cefoxitin and cycloserine is a selective and differential sheep blood medium used for the isolation of Clostridium difficile. Brucella agar is the nutritive base.

 

Brucella agar with hemin and vitamin K

Brucella agar with hemin and vitamin K is a general-purpose nonselective and enriched medium used for the isolation and cultivation of anaerobic bacteria.

 

Brucella agar with 5% horse blood

Horse blood enriches brucella agar for fastidious organisms, such as H. pylori, by providing both hemin (factor X) and NAD (factor V) factors.

 

Brucella broth

Brucella broth is a liquid medium that is used to cultivate Campylobacter species and to identify the organisms to the species level.

 

Buffered charcoal yeast extract (BCYE)

BCYE is a specialized enriched agar medium used for the isolation and cultivation of Legionella species from environmental and clinical specimens.

 

Buffered glycerol saline

Buffered glycerol saline is a multipurpose transport medium. The transport medium has been used for the isolation of bacteria, such as Aeromonas spp., as well as viruses. In addition, glycerol-containing media may also be used for longterm storage of isolates and for transport and storage of biopsy specimens.

 

Burkholderia cepacia selective agar

B. cepacia selective agar is an enriched and selective medium used for the isolation of B. cepacia.

 

Campylobacter blood agar

Campylobacter blood agar is an enriched selective blood agar medium used for the isolation of Campylobacter species.

 

Campylobacter charcoal differential (CCD) agar

CCD agar is a blood-free selective medium used for the isolation of Campylobacter from stool specimens.

 

Campylobacter thioglycolate medium

Campylobacter thioglycolate medium is a selective holding medium used for the isolation of Campylobacter species.

 

Cary-Blair transport medium

Cary-Blair transport medium was specifically designed to enhance the survival of enteric bacterial pathogens.

 

Cefoperazone-vancomycin-amphotericin B (CVA) medium

CVA medium is a selective and enriched blood agar medium used for the isolation of Campylobacter species.

 

Cefsulodin-Irgasan-novobiocin (CIN) medium (Yersinia selective agar)

CIN, or Yersinia selective agar, is a selective and differential medium used for the isolation and differentiation of Yersinia enterocolitica from clinical specimens and food sources.

 

Cetrimide agar

Cetrimide agar is a selective and differential medium used for the identification of Pseudomonas aeruginosa.

 

Charcoal selective medium

Charcoal selective medium is an enriched selective medium used for the isolation of Campylobacter species.

 

Chocolate agar

Chocolate agar is a general-purpose medium used for the isolation and detection of a wide variety of microorganisms, including fastidious species such as Neisseria and Haemophilus.

 

Chopped meat glucose broth

Chopped meat glucose broth is an enriched medium that supports the growth of most anaerobes. It is most commonly used to isolate Clostridium botulinum from mixed bacterial growth.

 

CHROMagar (Rambach agar)

CHROMagar is a microorganism-specific, chromogenic culture medium used for isolation and identification for a variety of organisms. CHROMagar was developed by Alain Rambach, who first formulated agars that were monochromogenic for the detection of E. coli and Salmonella spp. Second generation agars are multicolor. Both types are differential and selective. The nutritive agar base includes peptone and glucose. Different additives, proprietary chromogenic mixtures, and antibiotics have resulted in a series of media specific for such organisms as Listeria, S. aureus, methicillinresistant S. aureus (MRSA), E. coli O157, yeasts, and other organisms.

 

Columbia agar with 5% sheep blood

Columbia agar with 5% sheep blood is a general-purpose medium used for the isolation of a variety of microorganisms, including fastidious organisms. Addition of 20 gm of ampicillin per ml is helpful in isolation of Aeromonas.

 

Columbia broth

Columbia broth is a general-purpose clear liquid medium used especially for blood culture medium. The broth supports the growth of a wide range of microorganisms.

 

Columbia-colistin-nalidixic acid agar with 5% sheep blood

Columbia-colistin-nalidixic acid agar with 5% sheep blood is a selective and differential medium commonly used in the isolation of gram-positive aerobic and anaerobic organisms from mixed clinical specimens.

 

Cycloserine-cefoxitin-fructose agar

Cycloserine-cefoxitin-fructose agar is a selective and differential agar medium used for the isolation of C. difficile.

 

Cysteine-albumin broth with 20% glycerol

Cysteine-albumin broth with 20% glycerol is used for transport and storage of gastric biopsy specimens for the recovery of H. pylori.

 

Cystine glucose blood agar

Cystine glucose blood agar is an enriched medium used for the isolation of Francisella spp.

 

Cystine-tellurite blood agar

Cystine-tellurite blood agar is a modification of Tinsdale agar and is both a selective and differential medium used for the detection of C. diphtheriae.

 

Cystine tryptic agar

Cystine tryptic agar is a pancreatic digest of casein-enriched medium that is most commonly used for cultivation and maintenance of fastidious organisms. Addition of 1% carbohydrates and phenol red for determination of fermentation reactions is especially helpful for differentiating Neisseria spp.

 

Diagnostic sensitivity agar

Diagnostic sensitivity agar is a medium used in the cultivation of organisms for susceptibility testing.

 

DNA-toluidine blue agar

DNA-toluidine blue agar is a differential medium used most commonly for the detection and differentiation of Staphylococcus spp.

 

Dubos Tween-albumin broth

Dubos Tween-albumin broth is a nonselective medium used for the isolation and cultivation of mycobacteria.

 

Egg yolk agar (modified McClung-Toabe agar)

Egg yolk agar medium (modified McClung-Toabe agar) is a selective and differential medium used for the isolation and differentiation of Clostridium spp.

 

Ellinghausen-McCullough/Johnson-Harris medium

Ellinghausen-McCullough/Johnson-Harris medium is an enriched semisolid medium used for the isolation and cultivation of Leptospira.

 

Enterococcosel agar

It is similar to Bile esculin azide agar and broth.

 

Eosin-methylene blue (EMB) agar

EMB agar is a selective and differential medium used for the isolation and differentiation of enteric pathogens from contaminated clinical specimens.

 

ESP Culture System II

ESP Myco medium is used with ESP Culture System II (TREK Diagnostic Systems) for the detection of mycobacterial growth.

 

Egg-tellurite-glycine-pyruvate-agar (ETGPA)

It is similar to Baird-Parker agar base.

 

Fastidious anaerobic agar (Fusobacterium selective agar)

Fastidious anaerobic agar is an enriched sheep blood medium used for the isolation and cultivation of anaerobic organisms.

 

Fletcher’s medium

Fletcher’s medium is an enriched semisolid medium used for the isolation and growth of Leptospira.

 

FlexTrans viral and chlamydia transport medium

FlexTrans viral and chlamydia transport medium (Trinity Biotech) is intended to be used as a transport medium for viruses and/or chlamydiae.

 

GC agar base

GC agar base is a chocolate agar base used with various additives for the purpose of isolating Neisseria gonorrhoeae and other fastidious organisms, such as Haemophilus spp., including Haemophilus ducreyi, and for susceptibility testing of N. gonorrhoeae.

 

GN broth

GN broth is an enriched selective broth medium used for the isolation of gram-negative rods. Specifically, Salmonella and Shigella are isolated more effectively in GN broth than on solid medium alone.

 

Haemophilus test medium (HTM) and broth

HTM is an enriched medium used for susceptibility testing of Haemophilus species.

 

Heart infusion agar and broth

Heart infusion agar and broth are general-purpose media used for the isolation of a variety of microorganisms. Incorporation with 5% rabbit blood allows detection of the more fastidious Actinomyces.

 

Hektoen enteric agar

Hektoen enteric agar is a selective and differential medium used for the isolation and differentiation of enteric pathogens from contaminated clinical specimens. Fermenters such as E. coli produce colonies which are yellow-pink, Shigella spp. are green or transparent, and Salmonella spp. are green or transparent with black centers.

 

Hemin-supplemented egg yolk agar

It is similar to Neomycin egg yolk agar.

 

Isolator or lysis-centrifugation tube (Wampole Laboratories)

The Isolator is a unique system for the purpose of recovering organisms from blood through a simultaneous process of lysis and centrifugation. The system is especially good for the recovery of dimorphic fungi, yeasts, mycobacteria, and Bartonella spp. Recovery of anaerobes, Haemophilus spp., and pneumococci may be reduced.

 

Iso-Sensitest agar and broth

Iso-Sensitest agar and broth are media used for susceptibility testing in countries outside the United States.

 

John E. Martin Biological Enrichment Chamber (JEMBEC) (BBL) and InTray GC System transport medium (Biotest)

The JEMBEC and InTray GC devices are transport/inoculation media for direct plating of specimens for the detection of N. gonorrhoeae

 

Kanamycin-vancomycin laked sheep blood agar

Kanamycin-vancomycin laked sheep blood agar is an enriched, selective, and differential medium used for the isolation and cultivation of anaerobic bacteria, especially slowly growing and fastidious anaerobes from clinical specimens, such as Bacteroides spp. and Prevotella spp.

 

Lactobacillus MRS broth

Lactobacillus MRS (deMan, Rogosa, and Sharpe) broth is a nonselective liquid medium used for the isolation and cultivation of lactobacilli from clinical specimens and dairy and food products.

 

Levinthal agar with bacitracin and H. influenzae antiserum

It is similar to Chocolate agar.

 

Lim broth

Lim broth is a modification of Todd-Hewitt broth and is an enriched selective liquid medium used for the isolation and cultivation of Streptococcus agalactiae.

 

Lithium chloride-phenylethanolmoxalactam agar

Lithium chloride-phenylethanol-moxalactam agar is an enriched and selective agar used for the isolation and cultivation of Listeria monocytogenes.

 

Loeffler’s medium

Loeffler’s medium is an enriched nonselective medium used for the cultivation of corynebacteria, especially C. diphtheriae.

 

Lombard-Dowell egg yolk agar

It is similar to Neomycin egg yolk agar.

 

Lowenstein-Jensen medium

Lowenstein-Jensen medium is an enriched nonselective medium used for the isolation and cultivation of mycobacteria. It is similar to American Trudeau Society medium in its content and its ability to grow mycobacteria.

 

Lowenstein-Jensen medium (Gruft modification)

The Gruft modification of Lowenstein-Jensen medium is an enriched selective medium used for the isolation of mycobacteria. Penicillin and nalidixic acid are added to the medium and inhibit gram-positive and gram-negative organisms, respectively. RNA is added as a growth stimulant.

 

Lowenstein-Jensen medium (Mycobactosel modification)

The Mycobactosel modification of Lowenstein-Jensen medium is an enriched selective medium for the isolation of mycobacteria. Cycloheximide, lincomycin, and nalidixic acid inhibit saprophytic fungi, gram-positive organisms, and gramnegative organisms, respectively. No RNA is added.

 

Lowenstein-Jensen medium with 1% ferric ammonium citrate

Lowenstein-Jensen medium with 1% ferric ammonium citrate is an enriched and selective egg-based medium used for the recovery of Mycobacterium haemophilum. Ferric ammonium citrate is the additive which allows this organism to grow.

 

Lowenstein-Jensen medium with 5% NaCl

Lowenstein-Jensen medium with 5% NaCl is an enriched selective medium used to differentiate sodium chloridetolerant strains of Mycobacterium. Most rapid growers, e.g., the Mycobacterium fortuitum complex, as well as the more slowly growing organism Mycobacterium triviale, will grow on this medium.

 

Lysis-centrifugation tube

It is similar to Isolator.

 

MacConkey agar

MacConkey agar is a selective and differential medium used for the isolation of gram-negative organisms. The nutritive base includes a variety of peptones. The medium is made selective by the incorporation of bile (although at levels less than those used in other enteric media) and crystal violet, which inhibit gram-positive organisms, especially enterococci and staphylococci.

 

MacConkey agar with sorbitol (SMAC)

SMAC is a selective and differential medium used for the isolation and differentiation of sorbitol-negative E. coli.

 

MacConkey broth

MacConkey broth is a differential medium containing the indicator bromcresol purple used for the detection of coliform organisms from contaminated food, water, or stools.

 

Mannitol-egg yolk-polymyxin B agar

Mannitol-egg yolk-polymyxin B agar is an enriched, selective, and differential medium used for the isolation of B. cereus from mixed clinical specimens.

 

Mannitol salt agar

Mannitol salt agar is a selective and differential medium used for the isolation of S. aureus.

 

Martin-Lewis agar

Martin-Lewis agar is an enriched and selective medium for the isolation of N. gonorrhoeae.

 

MB/BacT ALERT (bioMèrieux)

MB/BacT ALERT contains a modified Middlebrook 7H9 medium supplemented with casein, bovine serum albumin, and catalase. It is used with MB/BacT ALERT 3D (bioMerieux, Inc.) for the cultivation and detection of mycobacterial growth.

 

MGIT (mycobacteria growth indicator tube) (BD Diagnostic Systems)

MGIT is a Middlebrook 7H9-based broth system that contains a fluorescence indicator, which is used for the detection of mycobacterial growth.

 

Middlebrook 7H10 agar

Middlebrook 7H10 agar is an enriched nonselective agarbased medium used for the isolation and cultivation of mycobacterial species.

 

Middlebrook 7H11 agar

Middlebrook 7H11 agar is a nonselective agar-based medium used for the isolation and cultivation of Mycobacterium species.

 

Middlebrook 7H9 broth with glycerol

Middlebrook 7H9 broth with glycerol is an enriched nonselective broth for the isolation of Mycobacterium species.

 

Mitchison 7H11 selective agar

Mitchison 7H11 selective agar is an enriched selective agarbased medium used for the isolation of Mycobacterium species.

 

Modified Irgasan-ticarcillin-potassium chromate broth

Modified Irgasan-ticarcillin-potassium chromate broth is a selective broth used for the isolation of Y. enterocolitica.

 

Modified Thayer-Martin agar

Modified Thayer-Martin agar is an enriched and selective agar for the isolation of pathogenic Neisseria species from clinical specimens with mixed flora.

 

Mueller-Hinton agar with and without 5% sheep blood

Mueller-Hinton agar with 5% sheep blood is used for susceptibility testing of S. pneumoniae. Mueller-Hinton agar with 5% chocolatized blood plus 1% IsoVitaleX and 3gm of vancomycin per ml is used for the isolation of H. ducreyi.

 

Mueller-Hinton agar with 2% NaCl

Mueller-Hinton agar with 2% NaCl is a selective medium used for testing the susceptibility of Staphylococcus to the penicillinase-resistant penicillins methicillin, nafcillin, and oxacillin by agar dilution or with the gradient-based system (E test).

 

Mueller-Hinton agar with 4% NaCl and 6 gm of oxacillin per ml

Mueller-Hinton agar with 4% NaCl and 6 gm of oxacillin per ml is the selective, differential medium used to screen S. aureus (not coagulase-negative staphylococci) for resistance to penicillinase-resistant penicillins (e.g., nafcillin, methicillin, and oxacillin).

 

Mueller-Hinton broth

Mueller-Hinton broth is a magnesium and calcium cation adjusted liquid medium used in procedures for susceptibility testing of aerobic gram-positive and gram-negative organisms by both macrodilution and microdilution methods.

 

Multiprobe media (M4-3, M5, and M4-RT) (Remel)

M4-3 contains vancomycin, amphotericin B, and colistin and is suitable for transport of viruses, chlamydiae, Mycoplasma, and Ureaplasma. M5 is similar to M4-3, but it does not contain gelatin. M5 is suitable for transport of viruses, chlamydiae, Mycoplasma, and Ureaplasma.

 

Mycobactosel agar

Mycobactosel is a BBL trade name for an enriched selective agar-based medium used for isolation of Mycobacterium species.

 

MycoTrim GU and MycoTrim RS (Irvine Scientific)

The MycoTrim GU and MycoTrim RS culture systems are unique triphasic flask systems specifically designed for the isolation and identification of M. hominis, U. urealyticum (GU), and Mycoplasma pneumoniae (RS).

 

NAG medium

NAG medium is an enriched and selective medium used for the isolation and cultivation of Haemophilus species from clinical specimens with mixed flora.

 

Neomycin egg yolk agar

Neomycin egg yolk agar is a selective and differential medium used for the differentiation of anaerobic organisms that are lipase positive, including Clostridium spp., Prevotella intermedia, Fusobacterium necrophorum, and some strains of Prevotella loescheii.

 

Neomycin-vancomycin agar

Neomycin-vancomycin agar is an enriched and selective medium that is particularly good for the isolation and cultivation of Fusobacterium from clinical specimens.

 

New York City medium

New York City medium is an enriched and selective medium for the isolation of pathogenic Neisseria from clinical specimens.

 

Nucleic acid transport (NAT) (Medical Packaging Corporation)

NAT is a nucleic acid transport device that is FDA cleared for use with multiple amplification and hybridization testing formats.

 

Oxford agar

Oxford agar is an enriched and selective medium used for the isolation of L. monocytogenes.

 

Oxidative-fermentative polymyxin B-bacitracinlactose agar

Oxidative-fermentative polymyxin B-bacitracin-lactose medium is a selective and differential medium used for the isolation of B. cepacia from respiratory specimens from patients with cystic fibrosis.

 

P agar

P agar is an enriched medium used for cultivation and isolation of staphylococci.

 

Peptone yeast extract broth

Peptone yeast extract broth is used in the analysis of metabolic products by gas-liquid chromatography because there is negligible acid volatility within the medium.

 

Petragnani medium

Petragnani medium is an egg-based medium. It is also used for the cultivation and maintenance of Mycobacterium smegmatis.

 

Phenylethyl alcohol agar

Phenylethyl alcohol agar is an enriched and selective blood agar medium used for the detection and isolation of anaerobic organisms, particularly fastidious and slowly growing bacteria, from clinical specimens with mixed flora.

 

PLM-5 TM

PLM-5 TM is a proprietary medium formulation (Intergen Co., Purchase, N.Y.) similar to Ellinghausen-McCullough/ Johnson-Harris medium that is used for the isolation and cultivation of Leptospira.

 

Polymyxin B-acriflavine-lithium chlorideceftazidime-esculin-mannitol (PALCAM) agar

PALCAM agar is an enriched, differential, and selective agar medium used for the isolation of L. monocytogenes.

 

Polymyxin B-lysozyme-EDTA-thallous acetate agar

Polymyxin B-lysozyme-EDTA-thallous acetate agar is a selective agar used for the isolation of B. anthracis from environmental specimens.

 

Polymyxin B-pyruvate-egg yolk-mannitolbromthymol blue agar

Polymyxin B-pyruvate-egg yolk-mannitol-bromthymol blue agar is an enriched, selective, and differential medium used for the isolation of B. cereus.

 

Polysorbate 80 medium

It is similar to Ellinghausen-McCullough/Johnson-Harris medium.

 

PRAS media

Prereduced anaerobically sterilized (PRAS) media are specifically manufactured (Anaerobe Systems) and packaged to eliminate oxygen to enhance growth of anaerobic organisms.

 

Pseudomonas cepacia (PC) agar

PC agar is a selective medium used for the isolation of B. cepacia (formerly Pseudomonas cepacia) from respiratory specimens from cystic fibrosis patients.

 

Rambach agar

It is similar to CHROMagar.

 

Rappaport-Vassiliadis enrichment broth

Rappaport-Vassiliadis enrichment broth is a selective and enriched broth used for the isolation and cultivation of Salmonella spp. from food and environmental specimens.

 

Regan-Lowe medium

Regan-Lowe medium is an enriched and selective medium used for the isolation of B. pertussis. Beef extract pancreatic digest, horse blood, and niacin are the nutritional base.

 

Salmonella-shigella agar

Salmonella-shigella agar is a selective and differential medium used for the isolation and differentiation of

Salmonella and Shigella from clinical specimens and other sources.

 

Schaedler’s agar

Schaedler’s agar is a general-purpose medium used for the isolation and cultivation of anaerobic bacteria.

 

Schleifer-Kramer agar

Schleifer-Kramer agar is a selective medium used for the isolation of Staphylococcus from heavily contaminated specimens such as feces.

 

Selenite broth

Selenite broth is an enrichment broth medium used for the isolation of Salmonella and Shigella species.

 

Sensitest agar

Sensitest agar is a medium used in susceptibility testing outside of the United States.

 

Septic-Chek biphasic mycobacterial media

The Septic-Chek system (Becton Dickinson Microbiology Systems) is a mycobacterial culture system which contains modified 7H9 broth and three types of solid media, modified Lowenstein-Jensen, Middlebrook 7H11, and chocolate agars, with various supplements.

 

Skirrow medium

Skirrow medium is an enriched selective blood agar medium used for the isolation of Campylobacter spp. from specimens with mixed flora.

 

STGG

STGG medium (skim milk, tryptone, glucose, and glycerin) is a transport medium that has been used for collection of nasopharyngeal swabs for the purposes of isolation and preservation of S. pneumoniae.

 

StrepB carrot broth kit (Hardy Diagnostics) and GBS broth (Northeast Laboratory Services)

Carrot broth and GBS broth are media that allow for the detection of red, red-orange, or orange pigment production by beta-hemolytic S. agalactiae (GBS) due to the hemolytic reaction with substrates such as starch, protease, peptone, and serum.

 

Streptococcus selective agar

Streptococcus selective agar is a selective medium for detection of streptococci. The agar base is Columbia agar.

 

Stuart’s transport media with and without charcoal

Stuart’s transport medium is an early transport medium first described in 1948. This medium uses glycerol phosphate to maintain the specimen as well as maintain the pH, agar, methylene blue as a redox indicator, and sodium thioglycolate to allow the survival of anaerobes.

 

Sucrose-phosphate-glutamate transport medium

Sucrose-phosphate-glutamate transport medium is used for the maintenance and transport of Chlamydia species and viruses.

 

2-Sucrose-phosphate transport media

2-Sucrose-phosphate medium is used for transport of specimens for the purpose of culturing Chlamydia trachomatis and Mycoplasma spp.

 

Tetrathionate broth base

Tetrathionate broth base is an enriched liquid medium used for the isolation of Salmonella species in contaminated clinical specimens and other products.

 

Thayer-Martin agar

Thayer-Martin agar is an enriched and selective medium used for the isolation of Neisseria from clinical specimens with mixed flora.

 

Thioglycolate with hemin and vitamin K

Thioglycolate broth with hemin and vitamin K is an enriched liquid medium used to support the growth of

microaerophilic and anaerobic organisms, including fastidious organisms.

 

Thiosulfate citrate bile salt sucrose (TCBS)

TCBS is a highly selective and differential medium for the recovery of Vibrio spp. except Vibrio hollisae and Vibrio cincinnatiensis.

 

Tinsdale agar

It is similar to Cystine-tellurite blood agar.

 

Todd-Hewitt broth with gentamicin and nalidixic acid

Todd-Hewitt broth is used for the isolation of beta-hemolytic streptococci from mixed flora, especially vaginal specimens for GBS.

 

Tryptic or Trypticase soy agar base with 5% sheep blood

Tryptic or Trypticase soy agar base with 5% sheep blood is a general-purpose medium used for the isolation of a wide variety of organisms.  The use of sheep blood provides an excellent means of interpretation of hemolytic reactions, especially those of Streptococcus spp.

 

Tryptic or Trypticase soy broth

Tryptic or Trypticase soy broth is a general-purpose clear liquid medium used for the cultivation of a wide variety of organisms. It is also recommended by the CLSI for preparation of an inoculum for Kirby-Bauer disk diffusion susceptibility testing and is the CLSI’s choice as a sterility testing medium. Formulations with 6.5% NaCl exist for the purposes of differentiating enterococcal species or salt-tolerant streptococci. Fildes enrichment is added to cultivate fastidious organisms such as Haemophilus spp.

 

Trypticase soy agar with horse or rabbit blood

Trypticase soy agar with horse or rabbit blood medium is used for the isolation of Haemophilus species.

 

University of Vermont modified listeria enrichment broth

University of Vermont modified listeria enrichment broth is an enriched and selective liquid medium used for the isolation of L. monocytogenes.

 

V agar

V agar is an enriched and selective medium used for the isolation of G. vaginalis from clinical specimens.

 

VMGA III medium

VMGA III (viability medium, Goteborg, anaerobic) is a transport and collection medium specifically designed to maintain viability of mixed anaerobes from peridontal and endodontal sites.

 

Wadowsky-Yee medium

It is similar to Buffered charcoal yeast extract (BCYE).

 

Wilkins-Chalgren broth and agar

Wilkins-Chalgren medium is recommended for susceptibility testing with anaerobic organisms. The medium contains specific nutrients that support the growth of anaerobes such as yeast extract, vitamin K, hemin, and arginine.

 

Xylose-lysine-desoxycholate (XLD) agar

Xylose-lysine-desoxycholate agar is a selective and differential medium used for the isolation and differentiation of enteric pathogens from clinical specimens. This medium is more supportive of fastidious enteric organisms such as Shigella. For Salmonella, which contains the lysine enzyme, this reaction reverts the pH to an alkaline state and the colony appears to be transparent or red with a black center. A number of other similar media for isolation of enteric pathogens exist, including xylose-galactosidase medium, which is more specific for Aeromonas spp.

 

Yersinia selective agar

It is similar to Cefsulodin-Irgasan-novobiocin medium.

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6 comments

  1. My name is Martin I am a biomedical technologists in rural Zambia am very passionate about medical microbiology and I recently set up the microbiology-bacteriology lab. I would be glad to do some research for interested parties…the area am in has high HIV related cases especially STIs

  2. Hii bro
    I am shahid having bachelor in microbiology and master in biotechnology. I am laso intrested in medical microbiology. Please share your experience and advise. Sahre new informations with through my personal email. I will also help you as much as i can.
    Thanks.

  3. Namaste Sir
    I’m Shital from Spain, doing lab techinican (final year), E.coli ko barema research gardai tiye, yo page ma aaipugye. Ekdam kushi lagyo, yesto ramro page vetda. 🙂
    Thank u so much for contents.

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