Lineweaver–Burk Plot
Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from a hyperbolic plot. Because of this difficulty, the Michaelis–Menten equation was transformed … Read more
Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.
Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from a hyperbolic plot. Because of this difficulty, the Michaelis–Menten equation was transformed … Read more
Eastern blot is a molecular biology technique that is used to detect post-translational modifications in proteins and the presence of components like lipids and carbohydrates. Eastern blot was discovered as … Read more
Sullivan and McCarthy’s Test Definition Sullivan and McCarthy’s test is a biochemical test for the detection of the amino acid methionine or methionine-containing proteins. The test is a specific test … Read more
Sakaguchi Test Definition Sakaguchi test is a biochemical test consisting of colorimetric reaction for the detection and quantification of guanidinium groups, used as a qualitative test for arginine that is … Read more
Pauly’s Test Definition Pauly’s test is a biochemical test for the detection of tyrosine and histidine where the reagent couples with amines, phenols, and imidazole groups. The test was discovered … Read more
Isatin Test Definition Isatin test is a biochemical test for the detection of amino acids like proline and hydroxyproline. The test is a specific test for specific amino acids that … Read more
Hopkin’s Cole Test Definition Hopkin’s Cole test is a specific test used for the detection of indole ring and thus, tryptophan in proteins. The test is also termed as ‘glyoxylic … Read more
Heat coagulation test of proteins definition Heat coagulation test of protein is a biochemical test performed to determine the presence of proteins like albumin and globulin in protein. Coagulation of … Read more
Ninhydrin Test Definition Ninhydrin test is a chemical test performed to detect the presence of ammonia, primary/secondary amines, or amino acids. This test involves the addition of ninhydrin reagent to … Read more
Tollens’ test is a chemical test used to differentiate reducing sugars from non-reducing sugars. This test is also called the silver mirror test based on the end product of this … Read more