Sagar Aryal, PhD

Sagar Aryal is a microbiologist and a scientific blogger. He completed his Ph.D. degree in Microbiology from the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal in 2025. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than 15 years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

DNA Sequencing- Definition, Principle, Steps, Types, Uses

July 16, 2024September 3, 2022 by Sagar Aryal, PhD

DNA sequencing refers to methods for determining the order of the nucleotide bases adenine,guanine,cytosine, and thymine in a molecule of DNA. The first DNA sequence was obtained by academic researchers, … Read more

Lac operon- Definition, structure, Inducers, diagram

September 5, 2022September 2, 2022 by Sagar Aryal, PhD

Lac operon definition The lactose or lac operon of Escherichia coli is a cluster of three structural genes encoding proteins involved in lactose metabolism and the sites on the DNA … Read more

Polymerase Chain Reaction (PCR)- Principle, Steps, Applications

September 5, 2022September 2, 2022 by Sagar Aryal, PhD

PCR is an enzymatic process in which a specific region of DNA is replicated over and over again to yield many copies of a particular sequence. The most widely used … Read more

Water Soluble Vitamins- B-Complex and C

September 5, 2022September 1, 2022 by Sagar Aryal, PhD

Vitamins refer to any of a group of organic compounds which are essential for normal growth and nutrition and are required in small quantities in the diet. Vitamins are biologically … Read more

Prokaryotic Transcription- Enzymes, Steps, Significance

September 5, 2022August 31, 2022 by Sagar Aryal, PhD

Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA). In prokaryotic organisms transcription occurs in three phases … Read more

Prokaryotic DNA Replication- Enzymes, Steps and Significance

September 5, 2022August 30, 2022 by Sagar Aryal, PhD

DNA replication is the process by which an organism duplicates its DNA into another copy that is passed on to daughter cells. Replication occurs before a cell divides to ensure that … Read more

Biochemical Test of Escherichia coli (E. coli)

September 25, 2022August 28, 2022 by Sagar Aryal, PhD

Biochemical Test of Escherichia coli (E. coli) Basic Characteristics Properties (E. coli) Capsule Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve) Flagella Flagellated Gas Positive (+ve) Gelatin Hydrolysis … Read more