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Sandeep Shakya

Sandeep Shakya is a biotechnology undergraduate student at Kathmandu University with academic training in microbiology, molecular biology, bioprocess engineering, and bioinformatics. His coursework and laboratory experience span bacterial and fungal culturing, biochemical testing, antimicrobial assays, PCR, gel electrophoresis, ELISA, rDNA technology, animal cell culture, plant tissue culture, and fermentation technology. Sandeep has participated in national and international research initiatives, including the Water and Food Security Biodiversity Innovative Challenge organized at Wageningen University, Netherlands, where his team secured second place among participating universities. His academic projects include water quality analysis using spectrometric techniques and applied laboratory investigations across environmental and medical biotechnology. In addition to laboratory science, he has experience in scientific design and communication, serving as a designer for his department’s magazine and leading bulletin board initiatives. He also holds Japanese language proficiency certification and demonstrates strong multilingual communication skills. Through Microbe Notes, Sandeep contributes structured, concept focused articles in microbiology and biotechnology, helping students understand laboratory techniques, molecular methods, and applied biological sciences in a clear and practical manner.

cDNA Synthesis from RNA: Principle, Steps, Uses

July 18, 2026June 24, 2026 by Sandeep Shakya
cDNA synthesis from RNA

The principle of cDNA synthesis revolves around using a DNA polymerase that can interpret an RNA template to generate a complementary DNA strand. Reverse transcriptase catalyzes the addition of deoxynucleotide … Read more

DNA Quantification Using a Spectrophotometer: Principle, Steps, Uses

July 11, 2026June 23, 2026 by Sandeep Shakya
DNA Quantification Using a Spectrophotometer

Accurate quantification of DNA is a fundamental requirement in molecular biology, biotechnology, and biomedical research. Many downstream applications, such as polymerase chain reaction (PCR), restriction digestion, cloning, sequencing, and next-generation … Read more

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