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Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

CLED Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal
Cystine Lactose Electrolyte Deficient (CLED) Agar

Cystine-Lactose-Electrolyte-Deficient (CLED) medium, first described by Sandysย and later modified by Mackey and Sandys,ย is generally used for diagnostic routine urinary bacteriology as a non-selective medium capable of supporting the growth of … Read more

Streptococcus Selective Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal
Streptococcus Selective Agar

Roantree et al introduced a medium for isolation of group A beta-hemolytic streptococci. The medium-enriched with yeast nucleic acid and maltose promoted increased colony size and enhanced clarity and sharpness … Read more

TCBS Agar- Composition, Principle, Preparation, Results, Uses

January 13, 2023January 2, 2022 by Sagar Aryal
Resultย Interpretation on Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar

Thiosulfate-citrate-bile salts-sucrose (TCBS)ย Agar, is a type of selective agar that is used in microbiology laboratories to isolateย Vibrioย species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae … Read more

Lactobacilli MRS Broth- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal
Lactobacilli MRS Broth

Lactobacilli MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium … Read more

Lysine Iron Agar Test- Principle, Media, Procedure, Results, Uses

January 8, 2022January 1, 2022 by Sagar Aryal
Result Interpretation ofย Lysine Iron Agar Test

Objective of Lysine Iron Agar Test To differentiate gram-negative bacilli based on decarboxylation or deamination of lysine and the formation of hydrogen sulfide (H2S). Principle of Lysine Iron Agar Test Lysine iron agar … Read more

Anaerobic Blood Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 1, 2022 by Sagar Aryal
Anaerobic Blood Agar

Anaerobic Blood Agar is a solid media recommended for use in qualitative procedures for primary isolation and cultivation of anaerobic organisms, including fastidious strains. This medium was formulated by V.R. … Read more

Czapek’s Agar (CZA)- Composition, Principle, Preparation, Results, Uses

May 4, 2022January 1, 2022 by Sagar Aryal
Czapek's Agar (CZA)

Czapek medium, also called Czapek’s agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It is recommended for use in qualitative procedures for the cultivation of saprophytic fungi, soil … Read more

Anthrax bacilli vs Anthracoid bacilli- 25 Differences

January 13, 2023January 1, 2022 by Sagar Aryal
Differences between Anthrax bacilli and Anthracoid bacilli

The saprophytic organisms which simulate the anthrax bacillus closely, both in their morphological and cultural characters within the group of Gram-positive aerobic sporing bacilli are termed as Anthracoid bacilli. They … Read more

Cystine Tryptic Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 1, 2022 by Sagar Aryal
Cystine Tryptic Agar

Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods … Read more

Peptone Water- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 1, 2022 by Sagar Aryal
Peptone Water

Peptone Water is formulated as per Shread, Donovan, and Lee. It is a broth medium used for the growth of the organism and a base for determining carbohydrate fermentation patterns … Read more

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