Sagar Aryal

Sagar Aryal is a microbiologist and a scientific blogger. He is doing his Ph.D. at the Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. He was awarded the DAAD Research Grant to conduct part of his Ph.D. research work for two years (2019-2021) at Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. Sagar is interested in research on actinobacteria, myxobacteria, and natural products. He is the Research Head of the Department of Natural Products, Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal. Sagar has more than ten years of experience in blogging, content writing, and SEO. Sagar was awarded the SfAM Communications Award 2015: Professional Communicator Category from the Society for Applied Microbiology (Now: Applied Microbiology International), Cambridge, United Kingdom (UK). Sagar is also the ASM Young Ambassador to Nepal for the American Society for Microbiology since 2023 onwards.

Cystine Glucose Blood Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal

Francisella tularensis on Cystine Glucose Blood Agar

Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., … Read more

New York City Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal

New York City Agar

NYC Agar Base medium was originally developed by Fauer, Weisburd, and Wilson at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens. … Read more

Deoxycholate Citrate Agar (DCA)- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal

Deoxycholate Citrate Agar (DCA)

Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of Salmonella and Shigella spp. This medium is similar to deoxycholate agar in comparison but … Read more

Sorbitol MacConkey Agar- Composition, Principle, Preparation, Results, Uses

January 8, 2022January 2, 2022 by Sagar Aryal

Sorbitol MacConkey Agar

MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting Escherichia coli serotype O157:H7 associated with … Read more

PDA- Composition, Principle, Preparation, Results, Uses

May 13, 2022January 2, 2022 by Sagar Aryal

Potato Dextrose Agar

What is a Potato Dextrose Agar (PDA)? Potato dextrose agar (PDA) is common microbiological basal growth media made from potato infusion, and dextrose is used for the growth of the fungi. Potato Dextrose Agar is recommended … Read more

Cary Blair Transport Medium- Composition, Principle, Preparation, Results, Uses

April 21, 2022January 2, 2022 by Sagar Aryal

Cary Blair Transport Medium

Cary-Blair Transport Medium is a simple, semi-solid, non-nutritive medium used for the collection and preservation of microbiological specimens. The minimal nutrients in the medium facilitate the survival of organisms without … Read more

Brain Heart Infusion (BHI) Agar- Composition, Principle, Preparation, Results, Uses

May 4, 2022January 2, 2022 by Sagar Aryal

Brain Heart Infusion (BHI) Agar

In 1919, Rosenow devised an excellent medium for culturing streptococci by using a dextrose broth supplemented with brain tissue. Rosenow’s formulation was later modified by Hayden who found the addition … Read more

O antigen and H antigen- Definition and 21 Key Differences

January 26, 2022January 2, 2022 by Sagar Aryal

Differences between O Antigen and H Antigen

Difference between O Antigen and H Antigen The surface structures of bacteria have considerable antigenic heterogeneity. Often these antigens are used as part of a serologic classification system for the … Read more

Chocolate Agar- Composition, Principle, Preparation, Results, Uses

January 13, 2023January 2, 2022 by Sagar Aryal

Chocolate Agar

Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base. As a result, the cell … Read more

CHROMagar- Composition, Principle, Preparation, Results, Uses

May 4, 2022January 2, 2022 by Sagar Aryal

CHROMagar (Rambach Agar)

The first chromogenic culture medium for the detection of E. coli was invented and patented by Dr. A. Rambach in 1979. This technology is a color-based differentiation method. It is … Read more