Fields of Microbiology
Microbiology is one of the most applied of all the biological sciences which did not exist as a true science before the later part of 19th century. It is defined … Read more
Sagar Aryal, PhD
Laboratory diagnosis of Legionella pneumophila
Laboratory diagnosis of Legionella pneumophila Specimen: Respiratory secretions (sputum, bronchial aspirate or washings), as well as pleural fluid, lung biopsy or autopsy material. Microscopy Legionellae in clinical specimens stain poorly … Read more
Bacterial growth curve and its significance
When a broth culture is inoculated with a small bacterial inoculum, the population size of the bacteria increases showing a classical pattern. When plotted on a graph, a distinct curve is … Read more
Epsilometer test (E test)- Principle, Procedure, Results, Advantages
Introduction Epsilometer test (E- test) is defined as the “exponential gradient” method that determines the antimicrobial resistance of the microorganism. It is a cost-effective tool that has been developed to … Read more
The Germ Theory of Disease
Before the Germ theory of disease, the causes suggested for the occurrence of disease were the effect of supernatural phenomena like planetary alignments, effect of bad bodily humors and the … Read more
Virus Cultivation- Purposes and Methods
Viruses are obligate intracellular parasites so they depend on host for their survival. They cannot be grown in non-living culture media or on agar plates alone, they must require living … Read more
Experiments in support and against Spontaneous Generation
Spontaneous generation is an obsolete theory which states that living organisms can originate from inanimate objects. The theory believed that dust created fleas, maggots arose from rotting meat, and bread … Read more
Gel Permeation Chromatography- Definition, Principle, Parts, Steps, Uses
Gel permeation chromatography is also called gel filtration or size exclusion chromatography. In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, … Read more
Robert Koch and Koch’s Postulates
Heinrich Hermann Robert Koch (1843 – 1910) provided remarkable contributions to the field of microbiology. He was a German general practitioner and a famous microbiologist. He is credited to be … Read more
History of Microbiology and Contributors in Microbiology
Varo and Columella in the first century BC postulated that diseases were caused by invisible beings (Animalia minuta) inhaled or ingested. Fracastorius of Verona (1546) proposed a Contagium vivum as … Read more