DNA Polymerase- definition, structure, types (vs RNA polymerase)

DNA polymerase

DNA polymerase definition DNA polymerases are a group of enzymes that are used to make copies of DNA templates, essentially used in DNA replication mechanisms. These enzymes make new copies of DNA from existing templates and also function by repairing the synthesized DNA to prevent mutations. DNA polymerase catalyzes the formation of the phosphodiester bond … Read more DNA Polymerase- definition, structure, types (vs RNA polymerase)

DNA Replication- definition, enzymes, steps, mechanism, diagram

DNA Replication

What is DNA? Deoxyribonucleic acid (DNA) is a nucleic acid that is made up of three components: a deoxyribose sugar, a phosphate, and a nitrogenous base. Deoxyribonucleic acid, DNA is the genetic material via which a cell is defined. It is a long molecule containing unique codes that give instructions for the synthesis of all … Read more DNA Replication- definition, enzymes, steps, mechanism, diagram

DNA- Structure, Properties, Types and Functions

DNA- Structure, Properties, Types and Functions

DNA stands for Deoxyribonucleic Acid which is a molecule that contains the instructions an organism needs to develop, live and reproduce. These instructions are found inside every cell and are passed down from parents to their children. It is a nucleic acid and is one of the four major types of macromolecules that are known to … Read more DNA- Structure, Properties, Types and Functions

30 Differences between DNA and RNA (DNA vs RNA)

Differences between DNA and RNA

Differences between DNA and RNA (DNA vs RNA) Some of the differences are: S.N. Character DNA RNA 1.       Full form Deoxyribonucleic Acid  Ribonucleic Acid 2.       Location DNA is found in the nucleus, with a small amount of DNA also present in mitochondria. RNA forms in the nucleolus, and then moves to specialized regions … Read more 30 Differences between DNA and RNA (DNA vs RNA)

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes The genome of an organism encompasses all of the genes of that organism. Gene is a distinct sequence of nucleotides forming part of a chromosome, the order of which determines the order of monomers in a polypeptide or nucleic acid molecule. Thus a protein-coding gene is defined as a region of … Read more Prokaryotic and Eukaryotic Chromosomes

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a … Read more Acridine Orange Stain- Principle, Procedure and Result Interpretation

DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications DNA fingerprinting or DNA profiling is a process used to determine the nucleotide sequence at a certain part of the DNA that is unique in all human beings.  The process of DNA fingerprinting was invented by Sir Alec Jeffrey at the University of Leicester in 1985. Principle of DNA Fingerprinting … Read more DNA Fingerprinting- Principle, Methods, Applications

Southern Blot- Principle, Steps and Applications

Southern Blot- Principle, Steps and Applications

The analytical technique that involves the transfer of a specific DNA, RNA or a protein separated on gel to a carrier membrane, for their detection or identification is termed as blotting. The process of transfer of the denatured fragments out of the gel and onto a carrier membrane makes it accessible for analysis using a … Read more Southern Blot- Principle, Steps and Applications

DNA Methylation

DNA methylation is a process by which methyl groups are added to the DNA molecule. It is an epigenetic mechanism that occurs by the addition of a methyl (-CH3) group to DNA, thereby often modifying the function of the genes and affecting gene expression.  The most widely characterized DNA methylation process is the covalent addition of the methyl group at the 5-carbon of … Read more DNA Methylation

Nucleosome Model of Chromosome

Nucleosome Model of Chromosome

Nucleosome Model of Chromosome The chromosomes are the nuclear components of the special organization, individuality, and function that are capable of self-reproduction and play a vital role in heredity, mutation, variation and evolutionary development of the species. Each chromosome is made up of DNA tightly coiled many times around proteins that support its structure. The proteins that bind … Read more Nucleosome Model of Chromosome