Deoxyribonuclease (DNase) Test- Principle, Procedure, Result, Uses

DNase Test Agar- Principle, Procedure and Result Interpretation

Deoxyribonuclease (DNase) Test Definition Deoxyribonuclease (DNase) Test is a biochemical test performed to differentiate organisms on the basis of their ability to produce the DNase enzyme. Deoxyribonucleic acid (DNA) is a large molecular-sized polymer composed of multiple nucleotides monomer that is large in size and thus, cannot enter the bacterial cell membrane. Microorganisms produce the deoxyribonuclease enzyme to breakdown the …

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DNA Polymerase- definition, structure, types (vs RNA polymerase)

DNA polymerase

DNA polymerase definition DNA polymerases are a group of enzymes that are used to make copies of DNA templates, essentially used in DNA replication mechanisms. These enzymes make new copies of DNA from existing templates and also function by repairing the synthesized DNA to prevent mutations. DNA polymerase catalyzes the formation of the phosphodiester bond which makes up the backbone …

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DNA Replication- definition, enzymes, steps, mechanism, diagram

DNA Replication

What is DNA? Deoxyribonucleic acid (DNA) is a nucleic acid that is made up of three components: a deoxyribose sugar, a phosphate, and a nitrogenous base. Deoxyribonucleic acid, DNA is the genetic material via which a cell is defined. It is a long molecule containing unique codes that give instructions for the synthesis of all body proteins. DNA structure The …

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30 Differences between DNA and RNA (DNA vs RNA)

Differences between DNA and RNA

Differences between DNA and RNA (DNA vs RNA) Some of the differences are: S.N. Character DNA RNA 1.       Full form Deoxyribonucleic Acid  Ribonucleic Acid 2.       Location DNA is found in the nucleus, with a small amount of DNA also present in mitochondria. RNA forms in the nucleolus, and then moves to specialized regions of the cytoplasm depending on …

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DNA Methylation

DNA methylation is a process by which methyl groups are added to the DNA molecule. It is an epigenetic mechanism that occurs by the addition of a methyl (-CH3) group to DNA, thereby often modifying the function of the genes and affecting gene expression.  The most widely characterized DNA methylation process is the covalent addition of the methyl group at the 5-carbon of the cytosine ring resulting in …

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Nucleosome Model of Chromosome

Nucleosome Model of Chromosome

Nucleosome Model of Chromosome The chromosomes are the nuclear components of the special organization, individuality, and function that are capable of self-reproduction and play a vital role in heredity, mutation, variation and evolutionary development of the species. Each chromosome is made up of DNA tightly coiled many times around proteins that support its structure. The proteins that bind to the DNA to form …

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Watson and Crick DNA Model

Watson and Crick DNA Model

Watson and Crick DNA Model DNA stands for Deoxyribonucleic acid which is a molecule that contains the instructions an organism needs to develop, live and reproduce. It is a type of nucleic acid and is one of the four major types of macromolecules that are known to be essential for all forms of life. DNA Model The three-dimensional structure of DNA, …

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Eukaryotic DNA Isolation Protocol

Eukaryotic DNA Isolation Protocol

Eukaryotic DNA Isolation Protocol Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. In eukaryotic cells, such as human and plant cells, DNA is organized as chromosomes in an organelle called the nucleus.  These cells also have a lipid bilayer outer …

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Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes The genome of an organism encompasses all of the genes of that organism. Gene is a distinct sequence of nucleotides forming part of a chromosome, the order of which determines the order of monomers in a polypeptide or nucleic acid molecule. Thus a protein-coding gene is defined as a region of DNA that encodes a single …

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Recombinant DNA Technology- Steps, Applications and Limitations

Recombinant DNA technology

Recombinant DNA Technology- Steps, Applications and Limitations Recombinant DNA technology refers to the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA (rDNA), on the other hand is the general name for a piece of DNA that has been created by the …

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