Nucleosome Model of Chromosome

Nucleosome Model of Chromosome

Nucleosome Model of Chromosome The chromosomes are the nuclear components of the special organization, individuality, and function that are capable of self-reproduction and play a vital role in heredity, mutation, variation and evolutionary development of the species. Each chromosome is made up of DNA tightly coiled many times around proteins that support its structure. The proteins that bind … Read moreNucleosome Model of Chromosome

Watson and Crick DNA Model

Watson and Crick DNA Model

Watson and Crick DNA Model DNA stands for Deoxyribonucleic acid which is a molecule that contains the instructions an organism needs to develop, live and reproduce. It is a type of nucleic acid and is one of the four major types of macromolecules that are known to be essential for all forms of life. DNA Model … Read moreWatson and Crick DNA Model

Eukaryotic DNA Isolation Protocol

Eukaryotic DNA Isolation Protocol

Eukaryotic DNA Isolation Protocol Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. In eukaryotic cells, such as human and plant cells, DNA is organized as chromosomes in an organelle called the nucleus.  These cells also … Read moreEukaryotic DNA Isolation Protocol

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes

Prokaryotic and Eukaryotic Chromosomes The genome of an organism encompasses all of the genes of that organism. Gene is a distinct sequence of nucleotides forming part of a chromosome, the order of which determines the order of monomers in a polypeptide or nucleic acid molecule. Thus a protein-coding gene is defined as a region of … Read moreProkaryotic and Eukaryotic Chromosomes

Recombinant DNA Technology- Steps, Applications and Limitations

Recombinant DNA technology

Recombinant DNA Technology- Steps, Applications and Limitations Recombinant DNA technology refers to the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA (rDNA), on the other hand is the general name for a piece of DNA that … Read moreRecombinant DNA Technology- Steps, Applications and Limitations

DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications

DNA Fingerprinting- Principle, Methods, Applications DNA fingerprinting or DNA profiling is a process used to determine the nucleotide sequence at a certain part of the DNA that is unique in all human beings.  The process of DNA fingerprinting was invented by Sir Alec Jeffrey at the University of Leicester in 1985. Principle of DNA Fingerprinting … Read moreDNA Fingerprinting- Principle, Methods, Applications

DNA- Structure, Properties, Types and Functions

DNA- Structure, Properties, Types and Functions

DNA- Structure, Properties, Types and Functions DNA stands for Deoxyribonucleic Acid which is a molecule that contains the instructions an organism needs to develop, live and reproduce. These instructions are found inside every cell, and are passed down from parents to their children. It is a nucleic acid and is one of the four major types … Read moreDNA- Structure, Properties, Types and Functions

Differences between DNA and RNA

Differences between DNA and RNA

Differences between DNA and RNA S.N. Character DNA RNA 1.       Full form Deoxyribonucleic Acid  Ribonucleic Acid 2.       Location DNA is found in the nucleus, with a small amount of DNA also present in mitochondria. RNA forms in the nucleolus, and then moves to specialized regions of the cytoplasm depending on the type of … Read moreDifferences between DNA and RNA

Southern Blot- Principle, Steps and Applications

Southern Blot- Principle, Steps and Applications

The analytical technique that involves the transfer of a specific DNA, RNA or a protein separated on gel to a carrier membrane, for their detection or identification is termed as blotting. The process of transfer of the denatured fragments out of the gel and onto a carrier membrane makes it accessible for analysis using a … Read moreSouthern Blot- Principle, Steps and Applications

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation Objectives of DNase Test Agar (DNA Hydrolysis) This test is used to differentiate organisms based on the production of deoxyribonuclease. It is used to distinguish Serratia spp (positive) from Enterobacter spp., Staphylococcus aureus (positive) from other species, and Moraxella catarrhalis (positive) from Neisseria sp. Principle of DNase Test Agar (DNA … Read moreDNase Test Agar- Principle, Procedure and Result Interpretation